High Remission Rates in a Brazilian Cohort of Initial Rheumatoid Arthritis after 15 Years of Follow-Up

Introduction: Rheumatoid arthritis (RA) is a chronic systemic rheumatic disease which is usually treated with corticosteroids and immunobiologicals. The goal of this article is to carry out an assessment of disease activity indices in a cohort of patients with rheumatoid arthritis. Patients and Methods: This is a prospective cohort study. Individuals from the Initial Rheumatoid Arthritis Brasília Cohort, which is an incident cohort of early RA diagnosed patients, were monitored at the Rheumatology Service of the Hospital Universitário de Brasília (HUB), University of Brasília (UnB), Brazil. A cross-sectional analysis was carried out from 2017 to 2018 to evaluate patients with 15 or more years of follow-up, through a direct interview and review of medical records. The main focus of the study is on the assessment of disease activity, based on the indices: 28-joint Disease Activity Score based on C-reactive protein (DAS 28 CPR) and based on erythrocyte sedimentation rate (DAS 28 ESR), Clinical Disease Activity Index (CDAI), and Simple Disease Activity Index (SDAI). The reference remission criteria used were the Composite Disease Activity Indices. Results: 107 patients were evaluated, mostly women, mean age of 55.1 who achieved higher rates of remission and lower disease activity than those reported in the literature.


Introduction
Rheumatoid arthritis (RA) is a chronic systemic rheumatic disease that affects approximately 0.5% to 1% of the population. The main symptoms are present in the peripheral synovial joints due to chronic inflammatory processes. Joint manifestations are generally symmetrical and preferably in the hands and feet. The most common manifestations are pain, edema, erythema, morning stiffness, deformities, and systemic symptoms that can affect different organs. The disease has a variable clinical course, from mild and self-limited to rapidly progressive, and is often characterized by systemic involvement. The disease limitations compromise the patient's quality of life [1] [2] [3].
RA has genetic and environmental components. The disease is probably caused by gene modifications that promote loss of tolerance to B and T lymphocytes, dendritic cells, and auto-antibodies, mainly anti-citrullinated protein antibodies, leading to inflammation, cartilaginous degradation, bone erosion, and, consequently, pain and other manifestations [3] [4].
The inflammatory process is already underway when the first symptoms appear. Therefore, the consequences of prolonged exposure to the harmful environment are less likely when the diagnosis is made quickly [4] [5].
Early diagnostic and therapeutic conducts and regular monitoring are important for a better quality of life. Early disease detection is important due to the window of greater responsiveness to the treatment. Regular monitoring improves adherence to therapy, generates patient confidence in the treatment, and aids management of the disease [6] [7] [8].
Patients who start early medical therapy have higher chances of returning to work in the long term and having a lower cost of living, in addition to less radiographic progression and higher statistics of disease remission [8] [9].
Remission, which is the main goal of treatment, is difficult to achieve even when using all the therapeutic arsenal available. The effectiveness of immunobiologicals, which are the most modern medications for treatment, vary between 10% and 22% according to the American College of Rheumatology 70% improvement criteria (ACR70) response assessment, which is an assessment criterion for improving the condition by 70% [10] [11].
The goal of this article is to carry out an assessment of disease activity indices in a cohort of patients with rheumatoid arthritis who have been regularly monitored since the onset of symptoms at a university hospital in Brazil.
H. L. Heller et al.

Patients and Method
This is a prospective cohort study, including individuals from the Initial Rheumatoid Arthritis Brasília Cohort [12] [13], which is an incident cohort of early RA diagnosed patients, who were monitored at the Rheumatology Service of the Hospital Universitário de Brasília (HUB), University of Brasília (UnB), Brazil.
For inclusion of patients in this cohort, RA is defined as the occurrence of joint symptoms compatible with inflammatory joint pain and edema, with or without morning stiffness or other manifestations suggestive of inflammatory joint disease, assessed by a single observer [14] [15] lasting more than 6 weeks and less than 12 months, regardless of meeting the American College of Rheumatology (ACR) criteria [16]. All selected patients met the EULAR (European Alliance of Associations for Rheumatology)/ACR 2010 criteria [17]. The patients had a medical diagnosis within 1 year or less of manifestation.
Patients received the standard therapeutic regimen of the service, including synthetic or biological Disease-Modifying Antirheumatic Drugs (DMARDs) as needed. A cross-sectional analysis was carried out from 2017 to 2018 to evaluate patients with 15 or more years of follow-up, through a direct interview and review of the medical records. A structured questionnaire was applied to each patient, obtaining information on sex, age, ethnicity, civil status, years of formal education, socioeconomic status, physical activity practice, the disease activity index (Disease Activity Score 28-DAS28) [18], functional disability questionnaire (Health Assessment Questionnaire-HAQ) [19], lifestyle habits such as To answer the objectives of the study, basic techniques of exploratory analysis were used, such as frequency and median analysis. P < 0.05 and 95% confidence intervals were considered significant. The analyses were conducted using the SAS 9.4 application.

Results
In total, 107 patients were evaluated, 95.3% female, with a mean age of 55.2 years (SD 13.13%). Regarding occupation, 39 patients worked with or without a formal contract (36.4%), 36 individuals declared themselves housewives (33.6%), 18 Open Journal of Rheumatology and Autoimmune Diseases (16.8%) were retired, 9 (8.4%) received sickness assistance or were dismissed by the National Social Security Institute, and 5 (4.6%) were unemployed. A total of 13 (12.14%) individuals changed their occupation due to RA and only 3 patients (2.8%) had health insurance. Furthermore, 29% of the patients did not present any comorbidity, 31.7% one comorbidity, 20.5% two, 10.2% three, 6.5% four, and 1.8% five or more comorbidities. Other socio-demographic characteristics, comorbidity specifications, the prevalence of alcoholism and smoking, and the frequency of physical activity are described in Table 1.
Concerning the disease characteristics, 75.5% of the patients were positive for rheumatoid factor and 12 (11.3%) had documented erosive disease. The mean HAQ at the time of assessment was 0.6 (median 0.35). The data were analyzed and classified as low, moderate, or high activity by the Composite Disease Activity Indices. The disease was classified as high activity index by the DAS28 CRP in only 3.1% of the patients, and most of the patients were in remission or had low activity of the disease. The DAS 28 (CRP and ESR), SDAI, CDAI data can be seen in Table 2.
Regarding treatment, 61.6% of patients (66 individuals) were using corticosteroids, 150 patients were using synthetic disease-modifying drugs-DMARDs (monotherapy or combinations), and 20 were using biological DMARDs; some patients were using more than one or two medications. Three patients (2.8%) were not using any medication for RA treatment at the time of data collection.
The frequency of use and dose of synthetic and biological DMARDs more commonly used in this cohort are summarized in Table 3 with the data from the most used treatment schemes.

Discussion
The socio-demographic characteristics found in this study are similar to those demonstrated in other groups of RA patients. The female sex has a significantly higher prevalence [20]- [25]. The average age of the patients presented a variation between 45 -57 years [20]- [25].
The ethnic variety is related to the territory which underwent a long process of miscegenation. In Latin America the phenotypes are very diverse [26]. The racial numbers found in this research corroborate with the Brazilian ethnic data, in which 47.7% of Brazilians consider themselves white and 43.4% brown [27].
With respect to education, as well as the NEW INDICES study [22] [23], in the current cohort most patients had low education, reflecting the average citizen treated in the Brazilian public system (SUS Sistema Único de Saúde) [28].
Regarding comorbidities, in a cohort conducted in Italy [22] [23], 27% of patients did not report any comorbidities, 25.5% only one comorbidity, 23.5% two, 10.7% three, 5.6% four, and 7.7% reported 5 or more comorbidities, which is similar to the data found in the current study.
Concerning the disease activity levels, a study based on the QUEST-RA database [25] [23] patients had a disease duration between 6.2 and 6.6 years.
The main limitation of this work refers to the type of study carried out. Since it is a cross-sectional analysis, it was not possible to carry out causality assessments between drugs and outcomes. In addition, the sample of patients analyzed was relatively small. Even so, the study brought an important conclusion that can serve as a basis for future research.

Conclusions
This study included a public service real-life scenario cohort setting in a developing country, whose population has low socioeconomic levels, and financial and structural limitations.
The cohort of patients with rheumatoid arthritis who started treatment at an early stage of the disease achieved higher rates of remission and lower disease activity than those reported in the literature.
Therefore, even in a real-life scenario with limitations, it is possible to obtain high rates of remission. Patient success can be justified by early diagnosis, targeted treatment, and continuous long-term monitoring of patients. A prompt approach to patients with symptoms suggestive of RA should be continually encouraged.

Conflicts of Interest
The authors declare no conflicts of interest.