Contribution to the Knowledge of Biodeteriogenic Flora on Three Historical Calabrian (Southern Italy) Churches

The effect of biodeteriogenic living organisms on historical and archeological sites is a well-known problem affecting the world’s cultural heritage. Indeed, the growth inside the bricks of shrubs and herbs roots can cause breakage, collapse and, detachment of materials. The knowledge of the spontaneous vascular flora present on monuments is essential for protection and safe-guarding of cultural heritage. In this paper, we identify and describe biodeteriogenic vascular flora of three historical Calabrian (Southern Italy) churches, showing the relationship between plant biodiversity and exposure and building material of churches. The species present on the wall and in perimeter for each church were sampled noting substrate type, surface inclination (vertical or horizontal) and exposure. The total number of plant species recorded was 27. Hemicryptophytes (41%) prevail over the other biological forms and the most represented families are that of the Asteraceae. Parietaria judaica is the only species present in all three sites. Substrate type, climatic condition and surface inclination affect the floristic composition. Sixteen out of 27 species grow on vertical surfaces, all Geophythes and Chamaephytes grow on horizontal surfaces, while Therophytes were rather on vertical surfaces. A significant difference was found in the Hazard Index values in the three churches. Only one site, presents a high average value of Hazard Index, due to the si-multaneous presence of Ailanthus altissima, Ficus carica, Rubus ulmifolius and Sambucus nigra. The data collected indicate that the flora growing on wall of three Calabrian churches partially reflects the floristic conservation interest, considering its biodeteriogenic potential in the evaluation of the impact of the vegetation on each monument.


Introduction
The biodeterioration of materials, defined as "any unwanted change in the properties of a material caused by the vital activity of organisms" [1] [2], is closely related both to the physical-chemical nature of the substrate and to the characteristics of the surrounding environment [3]. Therefore, it is possible to consider any artistic artifact as a real ecosystem in which there is a complex system of interactions between the organisms, the substrate on which they develop, and the environment [4].
Knowledge of flora growing on archaeological sites is extremely important both from a purely diagnostic point of view, which concerns the effects of alteration caused by biodeteriogenic organisms and from an ecological point of view, which considers the environmental context in which the monument is located.
That could allow the identification of the most appropriate actions to plan the correct conservation of the asset.
The colonization of stone monuments by an organism depends on specific conditions, such as the bioreceptivity of the building material itself which relies on its chemical-physical composition, porosity, roughness of the surface, wall inclination and exposure and its state of conservation [5]. Plant colonization of walls depends on physical environmental parameters too, such as temperature, light, humidity and air movements [6].
The vascular flora can have potential biodeteriogenic effects on historical buildings, which may bring about different types of damage to the substrate on which they grow, causing breakage, collapse and, detachment of materials, mainly as a result of biophysical and biochemical processes.
Usually, biodeterioration studies concern bacteria, fungi, and algae, but in recent years, great attention has been paid to mosses and vascular flora, due to the damage caused by rhizoids and roots growth inside the stone [7] [8] [9] [10] [11]. Indeed, a significant role in the biodeterioration of historical monuments is played by higher plants. Roots tend to penetrate and grow inside the cracks already present in the masonry and zones of the least resistance (e.g., mortar between stones), thereby increasing the size of the fissures and cracks and decreasing the cohesion between stones [5] [6] [8] [12]. Our work is focused on the study of vascular flora, so we collected trees, shrubs and herbs ignoring lichens and mosses. Moreover, the shape and the mode of growth of the root system are the factors on which the classification of the dangers of plant species for architectural artifacts is based [10] [13] [14].
The biochemical deterioration resulting from assimilatory processes, where the organism uses the stone surface as a source of nutrition, is probably more easily understood than the deterioration resulting from dissimilatory processes, where the organism produces a variety of metabolites that react chemically with the stone surface [15] [16].
Anatomical and morphological modifications of biodeteriogenic organisms are related to their physiological conditions, to the nature of the substrate, to the climate and the seasonal period [9] [17] [18].
The surveys designed to detect species that contribute to the deterioration of historic buildings are particularly valuable in the restoration and conservation of historical monuments and archaeological sites; however, at present, there are no studies applied to the evaluation of biodeteriogenic flora on the architectural heritage of Calabria.
The relationship between the vegetation growing in monumental environments and architectural artefacts is complex; it requires careful analysis in order to be managed correctly, respecting, on the one hand, the primary need for protection and conservation of the asset and, on the other hand, taking into consideration the aesthetic aspect linked to the presence of green and the ecological interest of the species that are present on the territory [19].
The aim of our study was therefore to identify and describe the vascular flora of three Calabrian historic churches, located in three geographic areas, with the aim of providing a plant species list, highlighting plant biodiversity, and considering its biodeteriogenic potential. We compared the effect of plants on ancient monuments based on their life form in relation to exposure and building material of churches. Moreover, we evaluated Hazard Index (HI) [13] [14], which is a measure of the potential damage that each species can cause to remains. This kind of information is necessary for the purposes of preserving the cultural landscape and for choosing appropriate management practices to prevent and eradicate vascular plants to minimise biodeterioration [20].

Study Sites
The three historic churches object of this study are located in the Calabrian region (southern Italy) ( Figure 1). Two churches are located on a hill at an alti-

Historical Background and Buildings Description
The Church of Santa Maria della Serra in Montalto Uffugo (SMS) (Figure 2(A)), dates back to 1227 and stands on a hill in a peripheral position from the center of the village of Montalto Uffugo. Over the centuries, it has undergone numerous restorations due to the disastrous earthquakes that damaged it over time.
The restoration work continued for about thirty years but left the upper part of the main facade incomplete, therefore it is made up of exposed bricks. The Church, originally in Romanesque style and initially composed of a Greek cross plan with three naves, later assumed a planimetric layout with Latin cross plants and architectural solutions in Baroque style [21]. The central portal is surmounted by a niche with the reproduction of the statue of the Madonna della Serra. The entire facade is decorated with a triumph of geometric and naturalistic embroidery.
The Mother Church of the Santissima Annunziata in San Fili (SA) ( Figure  2(B)) dominates the town and strongly characterizes the urban layout of the whole village of San Fili. The church has been documented since the seventeenth century and was enlarged at the end of the 18th century. The principal facade, unfinished in the upper part, is connected to the churchyard by a wide staircase leading to the portal. Today the church is divided into three naves; the main entrance consists of a round arch cleverly carved with floral motifs and acanthus leaves [21] [22].
Lastly, the Church of Santa Maria della Pietà in Squillace (SMP) (Figure 2(C)), so-called "Gothic church", is protected from urban and anthropic pollution and is not exposed to winds because it is sheltered by surrounding houses that shield it from the sun. It is one of the rare Gothic architectural artifacts in Calabria, dating back to the 13th century. It consists of an almost square plan. On the external facade exposed to the east, which is the only one of the four entirely visible as the others are occluded by an embankment, there are four single windows and a finely framed portal. Inside the building is divided into four spans with cross vaults supported by four semi-piers, four-corner columns and a large square central pillar having a very unique position because it is placed obliquely with respect to the perimeter of the walls. This building is erroneously called "church" but the real intended use of this building is not documented. Probably it's what remains of a "domus scolaciorum" of a civil-military character [23].

Substrates and Conservation Status
The facades of SMS and SA consist of white-yellowish (SA) or reddish (SMS) fossiliferous calcarenites [24] commonly known as "Mendicino limestone". This limestone, definite as biocalcarenite/calcirudite [25], is predominantly composed of sand-sized debris and carbonate grains consisting of fragments of older limestones and dolomites, other carbonate grains and clasts of biological origin such as marine organism fossils (corals, shells, ooids). It appears as a porous and medium-resistant material, chromatically variable depending on the more or less abundant presence of iron oxides [26].
The SMP building is mainly composed of carbonate rock characterized by a microsparitic texture rich in iron oxides, a medium-high level of porosity and a content of calcite higher than 90% [27]. The external wall structure is made up of irregular stone blocks, among which we can see fragments of terracotta. The mortars and plasters used for the construction and lining of the external walls have a very heterogeneous composition made mainly of quartz, feldspars, biotite and fragments of magmatic and metamorphic rocks.
The different building materials are linked to the geographical position; materials readily available in the vicinity were used for the construction of the churches.
For what concerns the state of conservation SMS has numerous spots affected by chromatic alteration, biological patinas, and black crusts in areas less exposed to rain on the main facade. At various points, especially under the cornice and on the sill of the central niche, there are phenomena of exfoliation and erosion of the masonry.
The church of SA presents an important form of chromatic alteration on the lower part of the main facade. The washout spots and biological patinas and black crusts are also conspicuous at various points. The upper part of the portal shows evident shortcomings and detachments of the masonry.
The SMP shows various pathologies of degradation, all of them due to the particular position of the building, which is located in a narrow blind lane and is surrounded by taller buildings: this makes the area not very sunny and ventilated. For the environmental conditions and for the characteristics of hygroscopicity and porosity typical of the materials used for its construction, inside the building the rate of relative humidity is very high throughout the year, as well as the ascent capillary water from the ground which, therefore, can be considered the main cause of all the deterioration phenomena present on the entire wall structure: masonry lesions, exfoliation, detachment, and sulphation of the plasters, salt efflorescence, biological attack and infesting vegetation.

Sampling
The field investigation for the detection of vascular plants present on the outside [31] and Tutin [32] with the method of the dichotomous keys for the taxonomic characterization. The nomenclature follows the checklist of Italian vascular flora [33] [34]. Families are organised based on APG IV for angiosperms [35].
To evaluate the hazard of the biodeteriogen species for the building, for each taxon the Hazard Index (HI) [13] [14] was evaluated. This numerical index, ranging from 0 (minimal hazard) to 10 (high hazard), differs for each species and is often variable even within the same species [7]. To obtain the HI of each church, the HI of different species within each sample was summed and the average was calculated for each church. According to Raunkier [36] the plant form life has been determined.  A difference in terms of number of plant species among sites was highlighted.

Result
More than half of the total species were found in the churches of SMS (12 taxa) and SMP (15 taxa   The Hazard Index (HI), used to evaluate the biodeteriogenic potential, showed that 18.5% of the species were not very hazardous (HI from 0 to 3), 66.7% were somewhat hazardous (HI 4 to 6), and 14.8% were very hazardous (HI 7 to 10). A significant difference was found in the HI values in the three sites examined ( Figure 5, Table 1). Indeed, only the church of SMP showed a high average value of Hazard Index (HI 6.3), due to the presence of 4 species with high values, Ailanthus altissima and Ficus carica (HI 10), Rubus ulmifolius and Sambucus nigra (HI 8). The other 2 churches, SMS and SA, showed much lower average values, 3.9 and 3.2, respectively: in these two sites 65% -75% of the species found had an intermediate HI value between 4 and 6.

Discussion
Our data indicate that the flora growing on wall of churches partially reflects the floristic context in which the churches are located, dominated by species associated with agicultural environment and natural areas. Indeed, most species are typically ruderal (Crepis neglecta, Diplotaxis tenuifolia, Picris hieracioides, Hordeum murinum, Parietaria judaica and Urtica dioica) and few are invasive (e.g. Ailanthus altissima, Mirabilis jalapa) [37].
The examined churches share the presence of some plant species suggesting that the different substrates of the walls (white-yellowish or reddish fossiliferous calcarenites and carbonate) do not influence the growth of plants.
Most species are spontaneously present on the walls and include trees (Ailan- and 6, respectively. They are almost all specimens with a generally not very developed root system, therefore they do not represent a big risk for the stability and the mechanical resistance of the walls.
Previous studies reported that horizontal surfaces, which provide better growing conditions, usually host a higher plant cover compared to vertical walls [38] [39]. In our case, more species were detected on vertical than on horizontal surfaces in agreement with Motti et al. 2020 [40]. However, in contrast to [40] and [41] in our sites Therophytes were more common on horizontal surfaces, while Geophythes and Chamaephytes, the most biodeteriogenic life forms, were on vertical surfaces.
The plant life forms revealed the presence of Hemicryptophytes and Therophytes in all three examined sites; the former were found predominantly on the walls while the latter are more abundant along the wall perimeter of the churches. Woody life forms accounted for only 25.9% in agreement with Francis [42], while the greater presence of Hemicryptophytes is related to human disturbance and climatic condition [7].
The largest number of species (15 taxa) was found on the site of Church of Santa Maria della Serra in Montalto Uffugo (SMP), which despite its small extension, presents a wide variety of biological forms with greater amounts of hemicriptophytes (6 taxa) and herbaceous, biennial or perennial plants. All vascular species with higher HI are also present on this church. In addition, there are non-native species of Italian flora that have become naturalized, and are all neophytes /invasive (Ailanthus altissima, Erigeron canadiensis, Mirabilis jalapa, Phytolacca americana and Sorghum halepense), probably related to the synanthropic environment represented by churches. There is a high variety of species typical of Mediterranean environments influenced by the proximity of the sea and by the climatic condition characterized by summer drought and moderate winter cold, with an average annual temperature of 15˚C.
Also in the site of SMS the total number of species (12) found is quite high; there is an equitable distribution of hemicyptophytes and therophytes (5 species) and also the only two detected Chamaephytes belong to this site. Erigeron canadiensis is the only naturalized neophyte species and Plantago lanceolata is a synanthropic species that adapts to almost all climates and soils. Most of the species found are annual and perennial herbaceous plants, except for two shrub species (Micromeria graeca and Trachelium caeruleum). The church is located in the center of the town of Montalto Uffugo but in a quite open area, free from obstacles, and with land around.
Finally, the church of the SA is the one where the least number of plants (5 taxa) have been collected. All the species identified are typically ruderal and weeds, with a value of HI between 0 and 5. The church is incorporated into the urban context and is enclosed by the surrounding buildings, which could explain the scarce variety of the species found. This is probably due to the difficulty for seeds to be conveyed in such narrow spaces and to settle on the facades and on the external perimeter.

Conclusions
The data collected contribute to the knowledge of the spontaneous vascular flora present on three historical Calabrian churches. Our data provided useful information on cultural heritage for understanding the relationship between plant biodiversity and abiotic factors (substrate type, climatic condition, exposure).
Our results confirm that plants constitute valid bioindicators of the "state of health" of the monuments. Due to their characteristics (life form, pollination and seed dispersal) and the Hazard Index suggestions, most of the listed plants are biodeteriogenic for these churches especially considering their building materials.
Information contained in this work can contribute to a better control of the growth of these species on church wall, since many of them are threatening the integrity of the ancient monuments due to their vigorous root apparatus penetrating into the brick structures. Finally, this knowledge can find applications in the conservation and restoration activities of ecclesiastical heritage.