GJB 2 Gene Related Nonsyndromic Hearing Loss in Mazandaran Province, North of Iran

Introduction: Congenital hearing loss is the most common sensory deficit in the world and mutations in GJB2 gene are the most common cause of deafness in many populations. Frequency of GJB2 mutations is estimated about 16% in Iran and varies among different provinces with a decreasing trend from north to south. The aim of this study was to investigate the frequency of GJB2 mutations in Mazandaran province, north of Iran, among non-syndromic hearing loss patients. Methods: 262 patients from 204 families participated in this study. After genomic DNA extraction, GJB2 gene analysis was carried out using DNA sequencing of both coding and non-coding regions by ABI 3130XL genetic analyzer. Results: 30.15% of all subjects showed mutations in GJB2 gene. Four mutations, including c.35delG (Gly12Valfs*), IVSI-1 + 1G > A, c.95G > A (Arg32His) and c.224 G > A (Arg75Gln) comprises 69.89% of all mutations in this study c.35delG and IVSI-1 were the most common mutations among patients respectively. Codon 75 mutation (c.224G > A. p: Arg75Gln) with autosomal dominant inheritance was seen in 7 cases from 3 families. 22 patients showed only one mutation in GJB2 gene and in 126 (48.09%) individuals, parents had a consanguineous marriage. Discussion: Frequency of GJB2 gene related hearing loss among patients was higher than average (16%) in this province. This study also showed a dominant inheritance pattern of GJB2 gene in this area. Consanguineous marriage also showed highly frequent among parents. More investigation needs to clarify cause of hearing loss in those 22 patients with one mutation in GJB2 gene, either two gene inheritance or another gene may be responsible for hearing loss.


Introduction
Deafness is one of the most common diseases in the world which is caused by hereditary or environmental factors or a combination of both [1] [2]. Congenital deafness has a prevalence of 1.3 per 1000 population and in 70% of cases is not associated with other clinical symptoms [3] [4] [5] [6]. Between 75% -80% cases of non-syndromic deafness are inherited in an autosomal recessive pattern.
Generally the frequency of hearing loss is reported 1 in 1000 live births in US and developed countries. The prevalence of deafness in Iran is higher due to the high rate of consanguineous marriages, approximately 3 per 1000 [7] [8]. This disorder is extremely heterogeneous and more than 150 genes are identified causing deafness to date. So far, 121 genes responsible for non-syndromic deafness have been identified, of wich76 genes have autosomal recessive inheritance, others have autosomal dominant (54 genes) and X-linked (5 genes) inheritance (http://hereditaryhearingloss.org). GJB2 gene, located at 13q12 and encodes Gap Junction beta2 protein, also known as connexin 26 protein and is necessary for cells to communicate with each other. This protein is localized in the cochlea and plays a role in k+ recycling pathway during auditory transduction [9] [10]. Different types of mutation in GJB2 gene such as Missense, Nonsense and frameshift are the most common cause of non-syndromic deafness with autosomal recessive inheritance pattern.
Although some mutations in this gene have been reported to be associated with autosomal dominant inheritance pattern [11]. So far, more than 300 pathogenic mutations have been reported for GJB2 gene that is associated with autosomal recessive non-syndromic deafness (http://databases.lovd.nl/shared/genes/GJB2).
The most frequent mutations in this gene is c.35delG that occurs due to a G deletion in a sequence of 6 guanines from position 30 -35 coding are and cause a frameshift and premature stop codon [11] [12] [13] [14].
The type and prevalence of mutations vary in different populations. The frequency of GJB2 mutations is reported from different provinces in Iran. It is generally as high as 22% to 38% in West Azarbaijan and East Azarbaijan province, Gilan and Golestan in the north and northwest provinces, to the average level of 16% in central provinces like Qom, Kerman, Isfahan, Yazd, Lorestan and as low as 4% to 0% in South Provinces like Sistan and Baluchestan [14] [15]. In general, it is estimated about 16% prevalence for GJB2 related hearing loss in Iran [14] [15]. Consanguineous marriage increases the frequency of homozygousity. It is estimated that 38% of the marriage is consanguineous and approximately 70% of cases are first cousins [16]. Also about 30,000 to 40,000 children are born every year with congenital disorders such as deafness [17] [18].

E. Hosseini et al. Open Journal of Genetics
Deaf persons often require different kinds of medical care, specialized education and social services, reduced work productivity which leads extra cost to the society. Although GJB2 mutations are highly frequent in Northern provinces in Iran (Golestan, Mazandaran and Gilan), there are very few previous reports about GJB2 gene mutation frequency from Mazandaran province. The aim of this study was to investigate the frequency of GJB2 mutations in Mazandaran province, which could be useful for genetic counseling and prenatal diagnosis.

Subject
This prospective study was recruited 262 patients from 204 families with hearing loss from Mazandaran province, north of Iran who referred to Novin genetic diagnostic laboratory and counseling center, during the 11-year period, 2009-2020).
After genetic counseling, family who was suspected to the hereditary form of hearing loss was included in this study. Any hearing loss with environmental cause was excluded from this study. All ethical issues, including confidentiality and informed consent were included in this study. All subjects were informed and were approved by the ethics committee of Mazandaran University of Medical Sciences.

DNA Extraction and PCR Amplification
A total of 5 to 10 ml of peripheral blood was collected in a EDTA container tube from each person, then genomic DNA was extracted according to established protocols [19] [20]. The quality and quantity of DNA samples were measured by Nanodrop spectrophotometer (Thermo Sci., Newington, NH). Two appropriate pairs of primers (Table 1) were applied (Macrogene, South chorea) to amplify exon 1 and exon 2 of GJB2 gene using polymerase chain reaction (PCR) followed by DNA sequencing method. A 850 bp and a 950 bp fragment was achieved by PCR amplification in a total volume of 25 μl respectively. Briefly, 2 μM of each primer (0.7 μl), 0.1 mM each dNTP (0.5 μl), 1× PCR buffer (2.5 μl), 1.5 mM MgCl 2 (0.7 μl), 0.5 unit of Taq polymerase (0.2 μl) and 19.3 μl distillated water (Cinnagen, Iran) were used in each PCR tube. The following amplification conditions: initially denatured at 95˚C for 5 minute, followed by 35 cycles of denaturation at 95˚C for 1 minute, annealing at 60˚C for1minute and extension at 72˚C for 1 minute and a final extension at 72˚C for 5 min. Quality of PCR product was checked by electrophoresis, 5-μl of each PCR product on was run on 1% agarose gel. Table 1. Specific primers to amplify exon 1 and exon 2 of the GJB2 gene.

DNA Sequencing and Genotype Analysis
Samples were subjected to further investigations using Sanger sequencing 3130XL Genetic Analyzers (ABI applied Biosystem, USA) for all individuals as a part of the diagnostic genetic laboratory services. In brief, specific primers were used to amplify entire exonic as well as 5' and 3' flanking intronic regions of the exon1 and exon2 of GJB2 gene, followed by DNA sequencing to find any variations or short deletion/insertions as described previously. Sequence analysis was performed using reference sequences from GenBank database. Gene Runner soft-

Statistical Analysis
Statistical analysis was performed using descriptive statistics. Allelic and genotype frequencies of the study variants were reported using basic descriptive statistics.  also were seen in this study with total frequency of 2.28%.

Results
Genotype composition and genotype frequency of the patients are shown in Table 4. As it is expected, homozygous patients for three most frequent recessive mutations are more common among patients (

Discussion
The GJB2 gene related hearing loss in Iran varies between 35% to zero, shows a decreasing trend from north to south of the country with average about 16% ac-    c.35delG was the most common GJB2 mutation accounted for 108 out of 226 (47.78%) mutated alleles in this study. c.35delG first was reported by Zlante et al.
IVSI + 1G > A is a splice site mutation in non-coding exon 1 of the GJB2 gene that was reported by Denoyelle et al. [38]. It has been found in several populations and frequent among Czech, Hungarian, Yakut and Turkish [39] [40]. This mutation is the third most common GJB2 mutation in Czech [39] and also it is reported 4.33% with total allele frequency in Turkish population [41] [42]. In Open Journal of Genetics Iran, the IVS1 + 1G > A is one of five most prevalent GJB2 mutations with frequency about 4.5% [23], with as high as 9.1%in Kurdish ethnic group [14] [43]. Results from this study also showed IVS1 + 1G > A as a second most common mutation in Mazandaran with the frequency of 14.45% (25 of 226) of the GJB2 mutated chromosomes.
c.95G > A (Arg32His) is a third most frequent mutation accounted for 10.4% (18 out of 173) mutated chromosomes in this study. Previous studies reported in one out of 70 consanguineous families from Isfahan, estimated 2.5% frequency in central area of Iran [44], 5 out of 1328 total allele tested (0.37%) in other study [25] and also 2 out of 131 (1.52%) in an unrelated nonsyndromic hearing loss samples from central part of Iran [1]. Results achieved from this study revealed slightly higher frequency of 3.43% (18 out of 524 alleles) from total chromosome studied. c.224G > A (Arg75Gln) cause autosomal dominant hearing loss [26]. In this study, 7 individuals from 3 families (7 out of 173 affected alleles) (4%) were seen with this mutation, with non-syndromic hearing loss ( For those 22 patients who showed only one mutation in GJB2 gene, a digenic inheritance could be involved or another gene rather than GJB2 may be responsible for hearing loss. Because hearing loss is heterogeneous, so it is a good candidate for digenic inheritance [46]. Several examples of digenic inheritance have been reported for hearing loss, such as PCDH15 and CDH23, SLC26A4 and FOX1, SLC26A4 and KCNJ10, MYO7A and PCDH15, PCDH and USH1G [47].
For GJB2 gene, different digenic inheritance including GJB2 and GJB6, GJB2 and GJB3, GJB2 and MIFT has been reported before [48]  gene is not responsible for the disease, another gene may involve, so analysis of all other known genes for hearing loss needs to be analyzed using next generation sequencing system.