Mycorrhizal Inoculation Effect on the Forage Cowpea Biomass Production in Burkina Faso

In Burkina Faso, breeding occupies an important place in the country’s economy, but is based essentially on the exploitation of the natural resources (pasture) which remains limited in extent due to the extension of land cultivated by agricultural practices extensive. Food is one of the most important limiting factors in animal production, hence the need to develop mechanisms to improve the production of food resources. This study is a contribution to fodder production improvement. In this study, cowpea was grown in a greenhouse for 60 days and inoculated with two inocula of arbuscular mycorrhizal fungi. The growth parameters were measured at 30 and 60 days after sowing. Shoot, root and total biomass were evaluated 60 days after sowing. The results show Yac 2 mix inoculum improves height growth (95.5%), height relative growth rate (525%), collar diameter (138%), collar diameter relative growth rate (328.57%), shoot biomass (396.3%), root biomass by (205.66%), total biomass by (320%), total nitrogen of aerial parts (92.39%) and total phosphorus of aerial parts (143.48%) compared to control. Yac 2 mix appears the most effective of all inoculum used in this study.


Introduction
In Burkina Faso, breeding is the third export product after gold and cotton. In a country with an agro-pastoral vocation, breeding occupies more than 80% of households in rural areas and constitutes the second productive sector in rural areas. It contributes 18% to gross domestic product (GDP) and constitutes a source of income for around 80% of the population [1]. However, an extensive type, this breeding is based essentially on natural resource exploitation. The major part of the food resources comes from natural pastures which, even if they are capable of improvement, remain nevertheless limited in extent due to the extension of the cultivated lands by extensive agricultural practices. In this country, the rainy season corresponding to the abundance of natural pastures remains very short. It varies from 3 months in the Sahelian zone to 5 months in the Sudanian zone [2]. Lack of fodder during the dry season is a major constraint on animal production, hence the need to find mechanisms to produce the quantity and quality of fodder. So, forage cowpeas could help improve forage production. However, in Burkina Faso, demographic pressure and the growing need to satisfy agricultural products are causing more and more intensive exploitation of natural resources (land, pastures). This overexploitation exceeds the capacity of the land which is characterized by soils lacking in nutrients, specifically nitrogen and assimilable phosphorus [3] with a low organic matter content, which shows the need to use fertilizers to ensure satisfactory and stable agricultural production. However, the use of chemical fertilizers has shown its limits due to pollution of soil and water, the appearance of pathogens resistant to pesticides, the high costs of pesticides, risks to the health of farmers and even embrittlement ecosystems [4]. Therefore, maintaining the productivity of crops by replacing the use of chemical fertilizers by mobilizing biological processes is at the heart of the challenges of current research.
However, cowpea [Vigna unguiculata (L.) Walp.] is a legume capable of associating with arbuscular mycorrhizal fungi (AMF) capable of improving their growth and production even when these plants grow on soils poor in mineral elements [5] [6] [7]. The exploitation of this symbiosis would be a possibility to improve the growth and production of cowpeas. Thus, this study was initiated with the aim of helping to improve its fodder production by mycorrhizal inoculation with Burkina Faso native arbuscular mycorrhizal fungi (AMF).

Plant and Fungal Materials
Seeds of Vigna unguiculata were used. The cowpea seeds were superficially disinfected by soaking in 96% ethanol for 3 min, rinsed thoroughly with sterile distilled water then disinfected in calcium hypochlorite solution (CaCl 2 O 2 at 3.3%, w/v) for 3 min and finally rinsed thoroughly with sterile distilled water before sowing. These seeds were then sown at a rate of 4 per pot.
Fungal material was composed of four local AMF isolated from the rhizosphere of cowpeas grown in Burkina Faso [8]: Yac 2 mix (Scutellospora sp., Gigaspora sp., Glomus sp.), Entrophospora sp., Gigaspora sp. and Glomus sp.  [8]. The inocula were obtained by multiplication of cowpea rhizosphere indigenous arbuscular mycorrhizal fungi [9]. Mycorrhizal inoculum production consisted of multiplying local AMF. Thus, maize (Zea mays) is grown in greenhouse in 4 liter pots containing 4 kg of sterile soil and inoculated with local AMF. The control pots also received 4 kg of sterile soil each, but were not inoculated. Each treatment is repeated 3 times and the test lasted 6 months. The inoculum is composed of a mixture of spores, mycorrhized roots fragments and soil. This inoculum is stored at 4˚C.

Test Implementation
The test consisted of growing cowpeas under greenhouse conditions (average temperature: 28˚C -30˚C; day length: 12 h) in 2 liter pots containing 2 kg of sterile culture substrate. The cowpea was sown at the rate of 4 seeds per pot and a wedge was carried out two weeks after the plants emergence to leave only one plant per pot. The inoculation was carried out at sowing time with 10 g of inocula (as described by Haro et al. [6]) per pot for each inoculated treatment.
Control pots were not inoculated. This device was composed of 5 treatments [four inoculated treatments (Yac 2 mix, Entrophospora sp., Gigaspora sp. and Glomus sp.) and one control] with 10 replicates per treatment has been retained.
The experimental design used was a simple randomization complete block design. This experiment lasted 60 days (flowering/fruiting stage).
To determine how inoculation affected cowpeas plant development, grow parameters, shoot, root and total biomass then aboveground partschemical characteristics were measured.

Grow Parameters Measurement
To estimate the effect of mycorrhizal inoculation on the cowpea, the height, the collar diameter, height relative growth rate and collar diameter relative growth rate were calculated at the 30 th and at the 60 th day after sowing. The height relative growth rate (RGRh) was calculated according to the formula (a) [

Shoot, Root and Total Biomass Measurement
At 60 days after sowing, each plant was carefully removed to recover the aerial part and all roots of the plants. These all parts were dried in 70˚C oven for 72 hours for shoot, root and total biomass measurement. After the biomass measurement, the roots were used for the mycorrhizal infection study. The chemical analysis (total nitrogen and total phosphorus) of cowpea aboveground parts was carried out by Bureau National des Sols (BUNASOLS) in Burkina Faso.

Staining for Mycorrhizal Colonization
About 10 g of roots from each treatment was thoroughly washed and placed in falcon tubes then cleared using 10% KOH. They were heated in 90˚C water bath for one hour. The roots were washed with tap water. Staining was then done by adding 0.05% trypan blue in lactic acid and heating in 90˚C water bath for 30 minutes as described by Phillips, Hayman [13] and observation was done under the microscope (magnification = 10×). The mycorrhizal frequency and intensity were estimated by Trouvelot et al. [14] method.

Statistical Analysis
Data were statistically analyzed using a one-way analysis of variance (ANOVA) with XLSTAT 2019 statistical software and the means were compared using the Newman-Keuls test (p < 5%).

Mycorrhization Frequency and Intensity Evaluation
The frequency and intensity of mycorrhization of cowpeas inoculated with the different local arbuscular mycorrhizal fungi (AMF) strain results (    The Yac 2 mix inoculum provides the highest values for height (40 cm), height relative growth rate (0.025 cm/day.cm), collar diameter (7.14 mm), collar diameter relative growth rate (0.03 cm/day.cm), shoot biomass (4.02 g), root biomass (1.62 g), total biomass (5.64 g), total nitrogen of aerial parts (1.77%) and

Efficiency of Native AMF Strains
total phosphorus of aerial parts (0.56 g·kg −1 ) of cowpeas.

Discussion
The mycorrhizal infection results show that the cowpea roots are strongly mycorrhized by the mycorrhizal strains used. These results are in agreement with those of Haro et al. [5] who showed that cowpea was strongly mycorrhized.
However, the mycorrhizal infection was absent on the control roots. This could be explained by the absence of any mycorrhizal contamination from the treatments. Similar results were obtained by Haro et al. [9]. The stimulation of growth parameters, biomass production and mineral elements content of cowpea between the different treatments and the control could be attributed to the AMF inoculation effect. At 30 days after sowing, inoculation did not improve either the height or the collar diameter (p > 0.5). This could be explained by the fact that the substrate used contains the necessary nutrients and directly accessible by the cowpeas roots proper nutrition. These results corroborate those of Haro et al. [9]. These authors have shown that since the symbiosis improves water and mineral nutrition, the plant will not find any need to form this symbiosis if the nutrients are available in the environment and directly accessible to the roots. However, with the plant evolution, the nutrients are gradually depleted in the soil and the roots can no longer meet the nutritional needs of the plant. Therefore, to fill this nutrient deficiency, the plant is forced to use the mycorrhizal symbiosis. Thus, for 60 days after sowing, significant differences (p < 0.05) appear between the different treatments for the height, the collar diameter, total nitrogen and total phosphorus of aerial parts, shoot, root and total biomass. The same is true for the height relative growth rate, collar diameter and collar diameter relative growth rate. Generally, all inocula used improved cowpea growth and biomass production. Also, apart from Glomus sp. inoculum which did not improve the phosphorus content of cowpea compared to the control, all inocula improved nitrogen and phosphorus content of cowpea aerial parts. These results could be explained by the effectiveness of the inocula used. Similar results are found by Haro et al. [9], Haro et al. [15], Haro et al. [16] who showed that mycorrhizal inoculation stimulated height growth of cowpea and that growth stimulation is more noticeable from the 35th day after sowing. Being the basic component of growth analysis that resulted from the work of de Blackman [12], the relative growth rate (RGR) of the plant or crop is defined at any instant in time (T) as the increase of the material present and is the only component of the growth analysis which does not require knowledge of the size of the assimilation system [11]. In this study, the height relative growth rate and collar diameter relative growth rate were used to assess the performance of mycorrhizal strains or the effects of mycorrhizal inoculation on plant growth. This made it possible to show that the plant growth variability observed in this study could be due to the difference in the AMF effectiveness in improving the mineral elements assimilation. As a result, Yac 2 mix appears to be more effective in improving cowpea growth. These results corroborate those of Haro et al. [17] who have shown the effectiveness of mycorrhizal symbiosis on improving the growth of Mucunapruriens. These authors also showed that this efficacy was linked to the mycorrhizal inoculum. Similar results were found by Haro et al. [18] on Zea mays. These results are confirmed by the phosphorus and nitrogen content of the aerial parts which are further improved by Yac 2 mix. Similar results were found by Haro et al. [6] on cowpea. Also, Ndonda et al. [19] have shown that mycorrhizal inoculation allows both increases in cassava yield, the availability of phosphorus formerly complexed by soil acid cations and the acceleration of the biosynthesis of the total carotenoid of yellow cassava.

Conclusions
In this study, it appears that the effect of inoculation is variable. Yac 2 mix is the most efficient of all the inocula used. This inoculum allows a considerable improvement in the production of cowpea biomass which can be used for the production of cowpea fodder. In addition to mycorrhizae, cowpeas also associate with soil bacteria (rhizobia). It would be interesting to extend this study by including rhizobial inoculations and the double mycorrhizal and rhizobial inoculations to assess the effect of these inoculations on cowpea biomass production.
The results obtained are promising, but this study was carried out under controlled conditions and on a sterile substrate. It would be interesting to extend this study in non-sterile soil and even in situ.