Sero-Prevalence of H. pylori Antibodies among Asymptomatic Rural Population in Bauchi State, Nigeria—A Preliminary Study

Helicobacter pylori infection is a major public health problem globally, with high prevalence in developing countries associated with poor sanitation, low standard of living, urban-rural disparity and increased gastrointestinal pathologies. This preliminary study determined the seroprevalence of H. pylori infection among asymptomatic rural population and association of sociodemographic variables on the result outcome. A total of 250 asymptomatic vo-lunteered participants were screened for H. pylori antibodies, using rapid immunochromatographic strips. 44.8% (112/250) were seropositive, and showed increased prevalence with the age group, <15 years (8.0%), 18 - 39 years (23.5%) and 40 - 65 years (12.0%)


Introduction
Helicobacter pylori infection is a major public health problem globally with increasing incidence of gastric and duodenal diseases [1] [2]. It is a gram-negative coccobacilli bacterium that colonises gastric mucosa and progress resulting in gastritis, duodenal ulcer, gastric ulcer, MALT lymphoma [3] [4]. The WHO reports that H. pylori virulence factors play a major causal role in the development of gastric cancer [5]. According to IACR, H. pylori is identified as group I carcinogen responsible for gastric cancer [6], which is the third most common cancer globally with high incidence in China and South Korea [7] [8] but low in sub-Saharan Africa with high seroprevalence [9] [10].
Globally, 50% of the world population is infected with H. pylori infection [1] [8]. In developing countries, acquisition of H. pylori infection occurs at childhood stage that may persist/or resolve due to cohort effect/or may progress into adulthood resulting in symptomatic manifestation, with the prevalence increased with age [8] [11] [12]. Substantial variations have been reported in H. pylori prevalence globally. There is high prevalence in developing countries ranging between 50% -90%, particularly among rural population [8] [13] [14] [15] in contrast to low prevalence of between 30% and 50% in developed countries [1], dependent on the level of urbanization, sanitation, degree of hygiene, socio-economic status, cultural practices of pre mastication of food, overcrowding due to living within a small space, and poor educational background [1] [8] [16].
The route of H. pylori transmission is still not clearly defined, but oral-oral, oral-fecal and intrafamilial are identified as major means of transmission [15] [16]. These are facilitated by exposure to risk factors such as low standard of hygiene, low living standard, poor sanitation, contaminated water, food overcrowding educational background socio-economic status and demographic variables [16] [17]. In Nigeria, serological screening of H. pylori antibodies in different population had revealed varied seroprevalence rates from as low as 15% in Maiduguri [18], 35% in Warri [19] to as high as 92% in Maiduguri [14].
Diagnostic methods for detection of H. pylori infection are classified into invasive and non-invasive. Invasive methods involve endoscopy procedure followed by histological analysis, culture, or PCR considered as "gold standard".
The non-invasive methods include serology, urea breath test and stool antigen and are mostly used for mass screening of H. pylori infection for epidemiological surveillance and evaluation of infection for eradication. The drawback of serological screening of H. pylori antibodies is possible overestimation of positive result. However serological method with enzyme immunosorbent assay method Open Journal of Gastroenterology of IgG gives comparative sensitivity and specificity level with other invasive methods [19] [20].
Bauchi state is one of the 6 administrative states in northeastern Nigeria, with no documented epidemiological data on H. pylori infection either at hospital or community setting, thus, creating an epidemiological information gap, in accessing the public health implication of the infection and template for eradication strategy. Although an unpublished data of dyspepsia patients who presented for endoscopic procedure per clinic day at gastroenterology/hepatology unit of Abubakar Tafawa Balewa Teaching Hospital, Bauchi (ATBUTH) indicated an upward trend over the years, majority of these patients resides outside the state capital, which point to possible increased seroprevalence of H. pylori in the rural area affirming the urban-rural population disparity of H. pylori infection [8] [21]. Baseline epidemiological information becomes imperative for public health assessment and template for possible treatment and management approach. Based on this information, we decided to conduct a preliminary study on serological screening of H. pylori antibodies among asymptomatic rural population and assess the association of sociodemographic variables on the result outcome in a rural settlement of Bauchi state, Nigeria.

Methodology
The descriptive cross-sectional study was conducted in a rural community of Faggo in Shira local government area of Bauchi state, between July and September 2018. Geographically, Faggo is located on 11'22˚ and 11'57' East, within the Sahel savanna region. Majority of the populace are mainly farmers engaged in petty farming and livestock rearing, while some few civil servants who work with the local government area. The settlement pattern is constituted of old architectural structures mixed with few modern structure, Basic amenity care facilities, like pipe born water and sanitation facilities are not adequate. Among the common cultural practices is the communal sharing of meals, eating from the same plate and sharing utensils. The study protocol was approved by Abubakar Tafawa Balewa Teaching Hospital (ATBUTH) institutional review board. Study participants recruitment was on voluntary basis, through the assistance of community and religious leaders involved in the sensitization, awareness and public health education on the importance of the study.

Data Analysis
Sociodemographic and seropositivity data were analyzed using SPSS version 21.0, in descriptive frequency with values expressed in means, frequency and percentage. Association between the categorical variable and seropositivity of H. pylori was determined by chi-square test. The level of significance was set at p < 0.05.

Result
The 250 asymptotic rural participants screened for H. pylori antibodies, com-

Discussion
Most documented studies of H. pylori infection had focused on symptomatic cases in urban population, with relatively few studies among rural population.
To our knowledge, this is the first report of serological screening of H. pylori antibodies among asymptomatic rural population in the study area. The study findings had provided a baseline data necessary for further studies aimed at better evaluation and possible eradication strategies. Open Journal of Gastroenterology  [29]. This observed variation in the prevalence may be due to geographical location, level of exposure to risk factors like poor standard of hygiene and sanitation, lower socio-economic status and detection method employed.
Association between sociodemographic variables and H. pylori infection have been well documented [8] [27]. Primarily, the H. pylori infection is acquired at childhood, persists in the system and progresses up to adulthood depending on the cohort effect and immune status of the individual, and the prevalence increased with age [8] [12] [15] [27] [30]. In our study, children < 15 years, seroprevalence increased from 8% to 23.6% among adult age group 18 -39 years and 12.0% among 40 -65 years, which is consistent with the findings in other studies [8] [31]. The prevalence level among children group in our study is lower when compared to other studies, 82.3% was reported among 5 -10 years subjects in Maiduguri [14], 68.2% -75.8% within age group of 2 -5 years and 14 -15 years in Benin republic [8]. However, similar low prevalence had been reported in other studies, 12.5% in Pakistan [17], 7% -33% in Oman [29] and 14% in Ghana [30]. This predisposes to intrafamilial transmission, particularly among mother and children (Alimajiri)-these are children seeking non-formal Islamic education [8] [17] [32] [33]. We are rather surprised by the low H. pylori seroprevalence recorded in our study area considering religious practice that allows young children to seek non-formal Islamic education which expose them to unhygienic environment, contaminated foods and water although such children did not feature among our study subjects and the design of the study did not allow for such details from the study questionnaire. However, as part of their culture engaged in communal eating with sharing of utensils. Despite, the predisposing risk factors the reason for low seroprevalence is rather unclear, which requires further evaluation.
There are documented data that support gender influence on H. pylori infection prevalence [8] [17] [19] [26] [28], which varies with study design and population. However, there is no biological evidence to support the gender difference. In this study, H. pylori prevalence of males was four times higher than females (35.2% vs 9.6%), with no significant difference, which is in agreement with other studies [8] [33]. In contrast, other studies had reported high prevalence among females [19] [29] The major reason that might be responsible for the high number of males study participants in our study, may be due to the fact that the males constituted the high population of study participants, which is also a reflection of socio-cultural and religious practices in the study area.
Socio-economic status and educational background of the study participants, are known indicators of public health education knowledge, awareness and preventive approach, associated with the level of good sanitation and personal hy-Open Journal of Gastroenterology giene [8] [17] [27]. In our study, significant association was observed between seropositivity and marital status (p < 0.010) and educational background (p < 0.051) of the study participants. Of the marital status, high seroprevalence was observed among married participants, 63 (25.2%) followed by singles 41 (16.6).
Common norm in the study area, is polygamy and large family size leaving within small household leading to overcrowding, communal living which involves sharing of households item that may facilitate intra-familial transmission of infection [12] [32], As documented in other studies, the common practice of pre-mastication of food for children by mothers can serve as means of transmission [32] [33]. The effect of overcrowding on H. pylori infection has been well documented, but its influence as a risk factor of H. pylori infection is dependent on socio-economic status of the family as low H. pylori prevalence is associated with high income family household [8].
In addition to socio-economic status, the level of education, particularly low educational background is associated with high prevalence of H. pylori infection [8] [17] [27]. In our study, significant association was observed between educational background of study participants and H. pylori infection (p < 0.05), with high prevalence recorded among study participants with non-formal educational background (60, 24.0%), consistent with other studies [8] [17] [27]. However, the level of educational attainment and H. pylori infection is dependent on exposure to the risk factors and socioeconomic status in the community Assessing the socio-economic status and H. pylori infection level, we determined the basic monthly income and H. pylori seropositivity, high seropositivity was recorded among non-salary earners 79 (31.6%), findings that are consistent with other studies [8] [27]. The findings are a reflection of socio-cultural practice that encourages polygamy and family living within small household that could encourage intrafamilial transmission and poor sanitation [8] [17] [27].
Though our study had focused on seroprevalence and sociodemographic factors, nevertheless, the findings had provided an insight on baseline epidemiological information of H. pylori infection among asymptomatic rural population that can serve as template for further studies. While this study had public health significance, there are limitations which include, non-evaluation of risk factors such as environmental and occupational activities that may impact the seroprevalence outcome and the serodetection technique employed in the study. As the ELISA assay of H. pylori IgG antibodies is known to give similar degree of sensitivity and specificity with the gold standard assay [20].

Conclusion
The H. pylori infection prevalence of 44.8% recorded in this study is relatively low in the community with high predisposing risk factors. This seroprevalence raises some epidemiological questions that required further comprehensive studies aimed at assessing risk factors of both environmental and occupational activities on seroprevalence of H. pylori.