Index Finger Reflection and Smoking

The reflectance of the index finger on 680 nm was measured. The experimental results show that the intensity of the reflectance for the index finger is related to age and the smoking circumstance. It was shown that reflectance for the index finger nonlinearly increases and reaches the maximum at about 31 - 32 age for nonsmoker age. Index finger reflectance between smoker and nonsmoker was compared. It was observed that smokers’ index finger reflectance is significantly greater compared with the index finger reflectance for nonsmokers.


Introduction
Skin is the boundary between the inside body and the environment. The optical properties of human skin have been of interest to researchers for Health some time. The interest was mainly based on a need to study a variety of different applications. In literature, one can find examples of researching reflectance on the skin on different parts of the human body, such as wrist skin [1] finger [2], inside of the forearm [3]. The skin reflection was intensively considered in order to help in the design and evaluation of the reflectance oxygen sensor [4].
The primary goal of this study is to investigate the differences in index finger skin reflection characteristics between smokers and nonsmokers, and also to find out of the obtained differences moderated by age. The results should give new insights into investigating the physiological and pathological condition of human body by considering the optical properties of the index finger skin.

Experimental
The object of measurement was the index finger, from the left human hand. Before measuring, the finger was washed in water and dried in hot air. The respective measurements of this work have been done in the following way: the fingertip has been lighted by LEDs of 680 nm ± 20 nm. The diode was turned on for 1 minute. During fingertip lighting, the intensity of the reflected light was measured with a photo detector-photodiode. The photodiode worked under the circuit regime, which enabled its stable operation in the surrounding temperature changes. The measurement geometry was always equal, unchanged throughout all measurements since the LED and the photodiode were fixed in the specially constructed hemisphere so that the angle between the incident and reflective radiation was 900. The signals from the photodiode were reinforced (10,000 times) and displayed. All results were normalized on the reflectance of standard MgO powder. Experimental setup is shown in Figure 1.
In the experiment, we have used convenience sampling method. There were more than 2000 participants in the experiment, all between 25 and 60 years old. which depend on one stochastic argument [5]. Therefore relative errors were less than ±2%.

Theory
There are a lot of examples in literature that prove that a drastic change in physiological and pathological conditions in skin or tissues causes changes in optical spectroscopic attributes. Sunburn skin of humans and animals show a difference in the intensity of fluorescence upon exposure to the sun [6]. Human skin shows a strong dependence of fluorescence intensity upon the activity of propionic bacteria acne [7]. The emission rate of a fingertip is strongly related with the age, the tumor presence, and lipid concentration in the serum [8]. A drastic change in steady-state fluorescence and excitation spectra from human native and malignant breast tissues was observed [9].
The mentioned discussion leads to the conclusion that smoking, as an unfa-

Results and Discussion
The result of measuring reflectance at 680 nm for several age groups was presented in Figure 2 and Figure 3. optical properties of skin, stimulated weak emission, upon age was observed [8].
Appearing maximum make that all reasonable explanations based on assumption that the significant changes in skin physiology and pathology due the year and, therefore in a change in their optical properties become unacceptable. Therefore, Figure 2. Dependence of the normalized reflectance (%) the human fingerprints upon age. rosis artery was found [13]. Also, laser-induced fluorescence shows a significant difference between human teeth with and without caries [14] and the difference between reflectance for cancerous and normal skin [15]. The obtained difference in reflection of the fingertip skin with the age of humans or in other words with the period of smoking is in agreement with literature data [16]. The smoking dose which is connecting with a period of consummations of cigarettes is related to skin pigment concentrations [17]. It is well known that the direct consequence of smoking is appearing reduction in arteries diameter [18]. Reducing the diameter of the arteries causes a decrease in the amount of blood that passes through the arteries and thus a decrease in the hemoglobin concentration.
Smaller arteries diameter can cause smaller blood flow and at this way smaller hemoglobin concentration. Therefore, a possible explanation for a significant increase in reflectance for smokers may be an insignificant decrease in the concentration of the hemoglobin concentration, which maximum absorbed at 680 nm, due to the decrease in arteries' diameter induced by nicotine [18]. Or in the simple relation: skin → smaller concentration of skin pigments → higher reflectance.
Taking the mentioned above into account, in our opinion, one reasonable explanation for the obtained change of the reflectance for the index finger (skin of index finger) could lie in the fact that the prolongation of the age of smoking increases the concentration of pigment in the skin and therefore the reflection. Or in the simple relation: skin → smaller concentration of skin pigments → higher reflectance. Chemicals in cigarette smoke cause the blood to thicken and form clots inside veins and arteries. Some experiments have shown that that cigarette smoke had an oxidative effect on the surface of the human skin [19]. Also, in literature data, one can find that smoking dramatically changes pigments which give the color to the skin exactly to the hemoglobin [20] and melanin [21]. On the other hand, some experiments have shown that cigarette smoking cause irregular pigmentation, and dryness of the skin [22] [23] [24]. Taking into account the mentioned above, a possible explanation in differences in reflections of fingertips between smoker and nonsmoker can be explained with a change in pigment concentration and skin quality. Also, with aging, the organism loses the ability to generi-cally defend itself from adverse effects, in this case, tobacco smoke.
In agreement with the mentioned above, it is clear that in order to make our research more precise and to obtain a real correlation between index finger reflectance and changing in arteries diameter, we must perform experiments using an electron microscope. This is the object of our further experiments.

Conclusion
Presented results lead to the conclusion that a direct connection between the physiological and pathological condition of human skin from a fingertip and the reflectance exists, and is moderated by age and smoking circumstance. Also,