Multiple Emulsions Able To Be Use For Oral Administration Of Actives Pharmaceutical Ingredients: Different Phases Physico-Chemical Parameters Study

When formulating emulsions, the preliminary tests, also known as formulation tests, constitute a step which can be long and expensive because of the quantity of reagents that can be used. A rigorous methodology could thus be of great interest, that is at the aim of our study which consists of evaluating the physico-chemical parameters of different phases used to make thus multiple emulsions. In our study, physico chemical parameters such as conductivity, pH, density, viscosity, and surface tension have been studied by direct measurement using equipment and also by means of suitable mounting. The results showed that the pH and the surface tension have an important role in the prediction of the stability of emulsions, these latter must be of the same order of magnitude. For all phases conductivity does not have too much interest apart from helping to determine the type of the emulsion. STATEMENT OF SIGNIFICANCE Multiple emulsions are of great therapeutic interest especially in the administration of medicament which can be inactivated by digestive enzymes, moreover the researches of formulation not being often easy, a control of the physicochemical parameters of the different phases would be of great interest in rapid formulations and at low cost.


STATEMENT OF SIGNIFICANCE
Multiple emulsions are of great therapeutic interest especially in the administration of medicament which can be inactivated by digestive enzymes, moreover the researches of formulation not being often easy, a control of the physicochemical parameters of the different phases would be of great interest in rapid formulations and at low cost.

INTRODUCTION
Emulsions are thermodynamically unstable systems, they are mixtures of two immiscible phases to be dispersed one within the other. The main goal is to keep this dispersion stable for a long time. In practice, according to Salager, "formulators of emulsions have experienced the unpleasant occurrence of the lack of reproduction of the physical properties (type, stability, viscosity) of an emulsion formulated with identical raw materials following the same rigorously definitive experimental protocol " [1,2]. Based on this assertion, we have studied the physical parameters which allow us to find information that is relevant for obtaining emulsions with good stability and which would be carried out within a rational time.
With regard to emulsions in general, many studies have been carried out on the various techniques which improve their quality and stability. These techniques are based on compositional, formulation and process variables [1,[3][4][5].
Concerning the formulation and composition variables, the one which were studied are the type of surfactant, the HLB, but also the proportions of the various constituents.   The following relationship determines the viscosity obtained using the limiting velocity reached by a moving particle in a viscous medium : η = 2(ρ drop − ρ medium ) 9. v limit . g. r drop 2  = viscosity, = density, v = velocity of the formed drop, r = radius of the formed drop, g = gravity acceleration.

Water in oil
Oil in water It was also studied the changes of the contact angles by observing the behavior of two superposed and not mixed phases [6].
The hydrophilic phase was mixed with 1% of CMC and with the emulsifiers constituted by the span 80 / tween 80 pair at 10%. The proportion of the mixture of Span® 80 / Tween® 80 which gave different HLB are indicated in table I.   It is noted a decrease in pH corresponding to an increase in the degree of acidity, a slight increase in conductivity and small fluctuations in the surface tension between 29 and 31 mN / m.

Study of the lipophilic phase
The results of pH measurements of the lipophilic phase, in which the proportions of emulsifying agent vary from 0 to 8%, are shown in the table IV below.

III DISCUSSION
The emulsions intended to do are W/O/W emulsions type [8]. For the realization we used three types of surfactants that allowed to vary the hydrophilic / lipophilic balance. These surfactants are, tween 80 and span 80, but also montane 481VG.
Data from the literature have shown that emulsifiers for water-in-oil emulsions must have an HLB between 1 and 6, hence the use of the montane 481VG which has HLB equal to 4.5, and for oil-in-water emulsions the HLB must be between 7 and 14 hence the use of span 80 tween 80 couple [3,9].
The study of the physico-chemical parameters such as pH, conductivity and surface tension allowed to see the variations of these properties as function of HLB especially with regard to the external hydrophilic phase. Since the goal was the formulation of multiple emulsions, the stability of the latter depends more on the external aqueous phase. For the latter, composition was of the utmost importance. Thus, as for the internal aqueous phase for which the pH is slightly acid in order of 6.8, the acidic pH is also observed for the external aqueous phase, which decreases as the HLB increases. The pH of the external hydrophilic phase to which we obtained a stable emulsion (HLB 8) of the order of 5.13 is quite close to that of the lipophilic phase with 6% emulsifier having a because an identical zeta potential can be observed around the droplets, which may be at the origin of a force of electrostatic repulsions between the droplets [10,11] We also noted that the HLB of span / tween 80 torque giving stable emulsions are the same as those found by ANKURMAN [12,13].
The most interesting parameter we have studied is the surface tension at the Indeed, by observing the curve of variation of the viscosity by means of the sedimentation rate of a drop of dispersed phase, it is observed that this velocity decreases and tends to stabilize around a value which,in regard to our emulsions, is 6%.

CONCLUSION
When making emulsions, the preliminary tests, also known as formulation tests, constitute a step which can be long and expensive because of the quantity of reagents that can be used. Therefore a good methodology could be of great interest what is at the origin of our study which consists of studying the physico chemical parameters of the different phases of the emulsions to be realized.
Thus the results of the study showed that the pH and the interfacial tension have an important role in the prediction of stability of emulsion, the interfacial tension of the two phases must be of the same order of magnitude. The measurement of the conductivity does not have too much interest apart from helping to determine the type of the emulsion.

AUTHOR CONTRIBUTIONS
All authors have contributed to the research and design of this item