Anatomical Structure of Aboveground and Underground Organs of the Rare Endemic Species Iris ( Juno ) magnifica vved., Growing under Natural Conditions of the Zeravshan Ridge, Samarkand Mountains

The morpho-anatomical structure of the aboveground and underground organs of the rare endemic species Iris (Juno) magnifica, growing under natural conditions of the Zeravshan ridge, Samarkand mountains, has been studied for the first time. A comparative analysis of the morpho-anatomical structure of the aboveground and underground organs revealed characteristic diagnostic signs of a microscopic structure. The complex of anatomical features of the aboveground and underground organs of the studied species are species-specific and can be used to solve taxonomic problems of this genus of plants, as well as the Red Book endemic species of juno irises, are of particular interest in connection with the potential for their vegetative reproduction.


Introduction
In modern taxonomy, both the understanding of the volume of the genus Iris, which provides for the inclusion of the genus of bulbous and tuberous species in the system, is widespread, and Iris determines only rhizome species in its composition. It is known that the anatomical signs of the leaf can be of great importance for the taxonomy of the genus Iris and the family Iridaceae [6] [7] [8] [9]. But the existence of phenotypic plasticity of anatomical features is also known, which depends on various factors, especially on the ecological conditions of the environment [11] [12] [13].
In the botanical literature in recent years, special attention has been paid to the structure of the epidermis and cuticle of the leaf. The shape of the cells of the leaf epidermis, the location, as well as the shape, location of the stomata are fairly constant generic and sometimes specific characters. Several environmental factors can affect the structure of the epidermis, without any connection with the genotype. For example, many authors believed that the size and frequency of stomata depend on environmental conditions [10] [11] [12] [13] [14].
T. Bacic [15] showed that the number and size of stomata varied depending on the influence of light and plant age. T. Nikolic [16] reports the phenotypic plasticity of the epidermal cell width caused by different lighting conditions. According to J. Pazourek [17], the stomatal frequency decreases with decreasing light intensity. G. N. Knecht and J. W. O'Leary [18] observed the development of more stomata with increased light intensity, T. W. Lucansky and K. D. Clough [19] pointed out the dependence of the anatomical features of the leaf on xeromorphism and mesomorphism of the habitat.
The Chinese and English scientists Qi-Gen Wu and D. F. Cutler [7] described the taxonomic, evolutionary and ecological significance of the leaf anatomy of 113 rhizome species and 20 intraspecific taxa from the first three subgenus Iris.
The taxonomic and ecological significance of structural characters was assessed and the relationship and evolutionary position of these taxa were studied. Some species with an uncertain taxonomic position are discussed and their classifica-N. K. Rakhimova et al. tion based on anatomical data is proposed. A correlation has been shown between some anatomical features and the type of habitat for certain types of irises.
Some xeromorphic and gelomorphic characteristics of the leaf are also given.
Croatian scientists B. Mitic and Z. Pavletic [20]  crystalline granules were also found. I. persica differs from the other taxa tested, but two species (I. peshmeniana and I. aucheri) showed closely related relationships.
We have previously studied the morpho-anatomical structure of the vegetative Anatomists and taxonomists of different countries are studying the anatomical structure of the aboveground and underground organs of Juno species to identify adaptive and diagnostic characters, which is of particular importance in the preservation of rare, endemic and endangered plants. Data on the anatomical structure of the aboveground and underground organs of the rare endemic species Iris (Juno) magnifica, growing in natural conditions in Uzbekistan, have not been studied. This determines the relevance and novelty of our research. The aim of the study is to study the anatomical structure of the aboveground and underground organs of the rare endemic species Iris (Juno) magnifica, in order to identify adaptive, diagnostic and taxonomic characters.

Materials and Methods
Iris (Juno) magnifica, listed in the Red Book of Uzbekistan, is a rare endemic of the Zeravshan Range with status 2 [28]. The perennial corm plant. The roots are strongly thickened, fusiform. The bulb 3 cm thick. Stem 25 -70 cm tall, with spread leaves; internodes are clearly visible. Leaves are light green, shiny, sickle-shaped, almost bordered along the edge, rough; lower 3 -5 cm wide. In cracks in rocks and on fine-earth areas among rocks in the lower belt of mountains. Samarkand region (Samarkand mountains). Ecology: In the middle belt of mountains. Habitat: Endem of the Zeravshan Ridge (Samarkand mountains).
The material was collected from the Zeravshan ridge, Takhta-Karacha pass, at an altitude of 4110 m above sea level. m. 39035'038'' s. 67 001'7396'' east longitude 04/16/2018. Taxonomic descriptions were made in accordance with the system of B. Mathew [29]. Morphological descriptions, ecology and area are given from "Flora of the USSR" and "Flora of Uzbekistan" by A.I. Vvedensky [30], the names of taxa are given from the database according to the site http://www.theplantlist.org: Samples of fresh plants were fixed in 70% alcohol solution. For the preparation of sections of vegetative organs, a manual method was used. The epidermis was studied on paradermal and cross sections. Paradermal sections of the leaf epidermis were prepared manually using forceps. The transverse sections of the leaf, sheath and stem were prepared by hand using a safety razor.
Cross-sections of the leaf and bulb are made through the middle, and the sheath of the leaf, stem and fleshy root through the base. Sections were stained with methylene blue, followed by gluing in glycerin-gelatin [31]. Descriptions of the main tissues and cells are given according to K. Esau [32], the epidermis according to N.A. Aneli [33]. Anatomical measurements were taken with a micrometer eyepiece attached to the microscope. The number of stomatal and epidermal cells was counted per 1 mm 2 of surface area. Various anatomical features of vegetative organs (leaf, leaf's vagina and stem): adaxial and abaxial sides of the leaf epidermis; mesophyll structure; the height of the epidermal cells; cuticle thickness; parenchyma cell diameter; diameter and number of vessels; length/width of stomata; submerged stomata; the number of layers of mesophyll cells; diameter and number of cell layers in the spongy, aquiferous and crustal parenchyma; height, width and number of layers of the palisade parenchyma; the diameter and number of layers of collenchymal and sclerenchymal cells were measured with a micrometer under a microscope.
The measurements were carried out depending on the organ, tissues and cells in 30-fold repetition with an eyepiece with a micrometer, followed by conversion to microns. Micrographs of anatomical signs of vegetative organs were made with a computer microphoto attachment with a digital camera brand A123 from Canon under a Motic B1-220A-3 microscope. Statistical processing of quantitative data was carried out in 30-fold repetition for each anatomical feature; the mean and the standard deviations of these measurements were calculated using a personal computer (MS-Excel program) according to generally accepted criteria [34].

The Structure of the Leaf
In the paradermal section, the basic cells of the epidermis, by the nature of the boundary walls, can be attributed to the rectilinear clan (Aneli, 1975). Epidermal cells belong to the rectangular type, in which rectangular cells are located along the vertical axis of the leaf.
Epidermal cells are most numerous on the abaxial side (there are 60.8 ± 0.72 of them per 1 mm 2 ), few on the adaxial side 41.8 ± 0.33. Leaves are hypostomatic. The form of the combination of stomatal cells (from the surface) is oval (36.4 ± 0.39 μm in length, 29.5 ± 0.09 μm in width), stomata are of the lenticular-equally thickened type [33], in which two identical semilunar cell shapes arranged symmetrically. On the frontal plane, the thickened shells are almost uniform. The slit is round. The stomata are anomocytic type, weakly submerged 9.3 ± 0.08 µm, there are 111.6 ± 2.17 of them per mm 2 ( Figure 1).
The leaf mesophyll on the cross section is of the isoform type, represented by spongy cells on both sides of the leaf and vascular-fibrous vascular bundles. The adaxial epidermis consists of one row of elongated cells with thick outer walls (7.4 ± 0.08 microns), the abaxial epidermis consists of rounded oval cells. Adaxial epidermal cells are large compared to abaxial ones (90.9 ± 1.37 -37.0 ± 0.32 μm in height). The spongy parenchyma consists of 7 -8 rows of large (31.5 ± 0.42 µm) small, chlorophyll-bearing rounded cells.
The main and lateral veins protrude on the abaxial side of the leaf, the vascular-fibrous vascular bundles are located in the central part of the leaf mesophyll. Under the abaxial epidermis and above the vascular-fibrous vascular bundles, there is an angular, large (7.5 ± 0.09 μm) 11 -14 row collenchyma. The main vein has 1 conductive bundle. Conducting bundles are closed, collateral, numerous, consisting of phloem and xylem, with 7 -8 small vessels (13.6 ± 0.09 µm) (Figure 1). American Journal of Plant Sciences

The Structure of the Leaf's Vagina
Sheaths of the sheet in a transverse section of the parenchymal-bundle type. The upper and lower epidermis consist of one row of rounded-oval cells 71.4 ± 0.62 -35.7 ± 0.34 μm in height, with thick outer walls 14.3 ± 0.08 μm. The cells of the abaxial epidermis are larger than those of the adaxial epidermis. The stomata are submerged -14.7 ± 0.07 microns. The parenchyma of the leaf's vagina consists of 10 -11 rows of large and small cells with a diameter of 64.8 ± 0.71 µm, rounded-oval. Vascular-fibrous vascular bundles are located in the center of the leaf's vagina and consist of phloem and xylem with 8-11 large vessels with a diameter of 28.6 ± 0.27 µm (Figure 2).

The Structure of the Stem
The base of the stem on the cross section is rounded, the structure of the parenchymal-bundle type. The epidermis consists of one row of rounded-oval-shaped cells 33.8 ± 0.35 μm in height, with a thin outer wall 6.3 ± 0.04 μm.
The primary cortex consists of 10 -12 rows of rounded-oval cells, with a diameter of 51.5 ± 0.53 µm, located between the epidermis and the central cylinder. The primary cortex is separated from the central cylinder by a ring of sclerenchyma. shows the rarity of this species (Figure 3).

The Structure of the Adventitious or Fleshy Roots
In monocotyledonous plants, in addition to the embryonic root of the embryo, during germination, adventitious roots immediately develop from the base of the stem (Figure 4).  On a transverse section, the adventitious or fleshy roots of Iris (Juno) magnifica is rounded with a diameter of 10 -11 mm, of a bundle-type structure and is divided into three parts: rhizoderm, primary crustal parenchyma and central cylinder. Rhizoderm consists of one row of tightly closed cells. The primary cortex consists of thin-walled, rounded-oval cells and is represented by three distinct layers: exoderm, mesoderm, and endoderm. The exoderm was located under the rhizoderm. Exoderm cells are polygonal, tightly closed, and consists of 4 -5 rows. Under the exoderm was the mesoderm (Figure 4, Figure 5).
The mesoderm consists of primary cortical parenchymal cells and is located outside of the endoderm. Consists of loosely located rounded-oval cells with a large diameter of 135.71 ± 1.41 μm, there are intercellular spaces, through which there is an intensive gas exchange. The cells of the cow parenchyma are large and they accumulate more nutrients The   Conducting bundles are closed concentric centroxylem type. The roots are characterized by alternation of the primary xylem and primary phloem areas in the central parts of the cylinder. Xylem forms a star, and phloem is located between its rays. The rays consist of 15 -16 rays of the primary xylem. In the very center of the cylinder there are 14 -15 small xylems with a diameter of 92.86 ± 0.93 microns, thick-walled sclerenchymal and parenchymal cells. Conductive bundles are sclerified due to sclerenchymal cells with a diameter of 21.43 ± 0.19 ( Figure 5).
When studying the anatomical structure, the following diagnostic signs were established for the adventitious or fleshy root: the root has a bundle type of structure and is divided into three parts: rhizoderm, primary crustal parenchyma and central cylinder. Rhizoderm is the primary integumentary tissue, consisting of one row of tightly closed cells. The primary cortex consists of thick-and thin-walled, rounded-oval cells and is represented by three distinct layers: exoderm, mesoderm and endoderm. Exoderm cells are oval, tightly closed. The mesoderm consists of primary parenchymal crustal cells, in which nutrients are accumulated. The central cylinder occupies the central part of the root. The pericycle consists of one row of oval-shaped cells. In the central part of the cylinder, there are conducting bundles consisting of phloem and xylem. Conducting bundles are closed concentric centroxylem type. The roots are characterized by alternation of the primary xylem and primary phloem areas in the central part of the cylinder. Xylem forms a star, and phloem is located between its rays. In the very center of the cylinder there are 14 -16 large xylems and thick-walled sclerenchymal and parenchymal cells. The conducting bundles are sclerified ( Figure 6).

The Structure of the Bulb
The bulb is an underground shoot with a short, flattened stem and fleshy, close, scaly leaves; stores water and nutrients (carbohydrates), serves for vegetative renewal and reproduction ( Figure 6).
The bulb is parenchymal-bundle type in cross section. Adaxial and abaxial epidermis consists of one row of rounded-oval cells. The height of epidermal cells is 28.6 ± 0.33 μm, with a thin-walled cuticle of 4.7 ± 0.03 μm. The cells of the adaxial and abaxial epidermis are large, elongated, with a thin outer cuticle wall.
The parenchyma of the bulb consists of 18 -20 rows of large and small round-oval cells with a diameter of 121.9 ± 1.3 μm. Among the parenchymal cells are hydrocytic cells. Parenchymal cells accumulate nutrients ( Figure 6).
Vascular-fibrous vascular bundles are located in the center of the bulb, not numerous, of a closed collateral type, consisting of phloem and xylem, with 6 -7 large and small vessels with a diameter of 27.8 ± 0.25 µm. Calcium oxalate crystals occur between parenchymal cells ( Figure 6).
When studying the anatomical structure of the bulb, the following diagnostic signs were established: the bulb has a bundle type of structure; adaxial and abaxial epidermis consists of one row of rounded-oval cells. Parenchymal cells are thin-walled, large and small-celled, multi-row, and they accumulate a large number of nutrients. Vascular-fibrous vascular bundles are located in the center, closed collateral type, consisting of phloem and xylem, with 4 -5 large and small vessels ( Figure 6). American Journal of Plant Sciences

Conclusion
Thus, a comparative morphological and anatomical structure of the aboveground and underground organs of Iris (Juno) magnifica was carried out. Diagnostic signs of microscopic structure were determined: the basic cells of the leaf epidermis by the nature of the boundary walls belong to the rectilinear clan to the rectangular-walled type. Leaves are hypostomatic. The form of the combination of stomatal cells is oval, the stomata are of the lenticular-equally thickened type. The mesophyll of the leaf on the cross section is of the isoform type of structure, represented by spongy cells on both sides of the leaf and vascular fibrous vascular bundles. Under the abaxial epidermis and above the vascular-fibrous vascular bundles, there is a corner collenchyma. In the main vein of the leaf, there is 1 closed, collateral-type conducting bundle. The sheaths of the leaf and the base of the stem on the cross section are of the parenchymal-bundle type. The primary bark of the stem is separated from the central cylinder by a ring of sclerenchyma. On the periphery of the stem, under the sclerenchymal cells, there are conductive bundles of a closed collateral type. The anatomical structure, the following diagnostic signs were established for the adventitious or fleshy root: the root has a bundle type of structure and is divided into three parts: rhizoderm, primary crustal parenchyma and central cylinder. Conducting bundles are a closed concentric centroxylem type. The roots are characterized by alternation of the primary xylem and primary phloem areas in the central part of the cylinder. Xylem forms a star, and phloem is located between its rays. The anatomical structure of the bulb and the following diagnostic signs were established: the bulb has a bundle type of structure. Parenchymal cells accumulate a large number of nutrients. Vascular-fibrous vascular bundles are located in the center, closed collateral type. The complex of anatomical features of the aboveground and underground organs of each of the studied species of Juno irises are species-specific and can be used to solve taxonomic problems of this genus of plants. The studied endemic species of Juno irises from the Red Data Book are of particular interest due to the potential for their vegetative reproduction.