Stem Population and Tissue Replacement of Urochloa in Different Phenological Stages

The objective of the present investigation was to evaluate the Urochloa Insurgent, Piata and Signal grasses by varying the phenology in the attributes: stem population dynamics, tissue replacement, leaf:stem relation and weight per stem. The data were analyzed using a completely randomized block design with arrangement in divided plots and four replications, the procedure used was PROC GLM from SAS. The Signal grass presented higher stem density with an average of 450 m −2 stems, while the meadow with Insurgent grass registered the lowest stem density throughout the investigation, with an average of 320 m −2 stems; furthermore, in this treatment, the stem density tended to increase slowly over time (P = 0.05). The Insurgente and signal grasses showed rapid leaf elongation from day 21 of regrowth with 123 and 104 cm stem −1 , while in Piata the accelerated leaf elongation was on day 14 with 113 cm stem −1 where it remained active its growth until day 56 (P > 0.05). As the regrowth age was increasing, the population dynamics of stems and weight per stem were increasing; in Insurgent and signal the leaf elongation and net growth increased to a maximum point to start declining; howev-er, in Piata grass was increasing without decreasing. The trend of the leaf:stem relation was decreasing as the test progressed and senescence increased from day 21 of regrowth.

In most ruminant producing tropical areas, they suffer nutritional stress either temporarily or permanently [8]. The hybrid forages of Brachiaria now Urochloa are originated from Africa and are considered an alternative that meets basic nutritional requirements, providing better quality dry matter [9] [10], compared to native forages, mainly for the dry season.
Studies indicate that these species have been used for in vivo investigations in cattle [11] with positive results; however, management is essential in the persistence of the meadow, which determines the intensity and frequency of cutting or grazing [12], components that modify the animal load, according to the degree of defoliation [13] [14]. Other researchers [15], evaluated different frequencies in Mulato grass (Brachiaria hybrid 36061) and mention that in the rainy season at 21 and 28 days the highest forage production, growth rate and leaf production were obtained. In Cobra grass (Brachiaria HYBRID BR02/1794), the growth curve was evaluated, determining that 35 days after regrowth it has higher quality and quantity of leaf [16].
Knowledge of the density, weight of the stems and elongation of leaves provides an essential reference for the management of meadow [17]. There are studies where B. brizantha cv, Marandu, B. decumbens and B. ruziziensis have been evaluated; however, it is necessary to investigate the Piata, Signal and Insurgent cultivars, from the basics such as yield, growth dynamics and quality, cultivated alone and in associations [18] [19]. Therefore, the objective of the present investigation was to evaluate the Urochloa Insurgent, Piata and Signal grasses by varying the phenology in the attributes: stem population dynamics, tissue replacement, leaf:stem relation and weight per stem.

Location of the Experimental Area
The present investigation was carried out in the experimental unit of the Instituto Tecnológico de Pinotepa, located at kilometer 26 of the Pinotepa Nacional highway, Oaxaca-Acapulco, south of the town of San José Estancia Grande, Oaxaca, in the period from August to December from 2017 and laboratory analyzes in the same experimental unit from January to June 2018.
It is located at 16˚22' north latitude and 98˚13' west longitude, with a height of 60 masl according to the Köeppen climate classification, it presents an Aw (w) ig climate, it corresponds to a sub-humid warm climate [20], with a rainy season in summer. The pluvial precipitation is in a range of 1000 -1500 mm annually with a rainy period that includes from June to September with 8 months of drought, American Journal of Plant Sciences with an average temperature of 26˚C -28˚C.

Plots Management
The sowing was carried out on August 10, 2017, with pure viable seed from three grasses of the Urochloa genus. The sowing method was direct, at a distance between furrow and furrow of 50 cm and between plants of 20 cm. Four 10 × 5 m plots were sowed, being experimental units for each grass. These are in turn in eight areas, in order to evaluate a growth analysis with eight regrowth ages. The soil with a sandy clay texture, pH 4.8 to 5.0, deficient in organic matter. The weed was controlled manually with the help of a hoe, two nitrogen fertilizations were carried out with urea (46-00-00), the first was done on September 28 and the second was on October 17, 2017. On October 12, 2017, a homogenization cut was made at a height of 10 cm in all the experimental plots. Irrigation was carried out every 3 days by the drip method from the date of the cut until completed the investigation.

Stem Population Dynamics
To determine the changes in the density of stems, at the beginning of the experiment, two PVC rings of 10.4 cm diameter were placed in each experimental unit, which delimited a tiller. All the stems present inside the ring were marked with cable rings of the same color, which were considered as the initial population. Subsequently, each week for eight weeks, the new stems were marked with different colored rings; a different color was used for each generation, and the dead stems were counted and the ring was removed.

Tissue Replacement
Total growth, net growth and foliar senescence were evaluated in a 2 m long transept, five stems of each grass were randomly selected and identified with wire rings of different color. Stems measured: the length of the foliar blade, from the base of the ligule to the apex in green leaves or to the base of the chlorotic tissue in senescent leaves. A linear regression equation was obtained considering the foliar area as the dependent variable and the rib length as the independent variable [21].

Leaf:Stem Relation
The leaf:stem relation data was obtained by cutting two 50 × 50 cm squares weekly in each experimental plot, leaving 10 cm of remaining foliar area; separating into leaf and stem, and it was deposited in a forced air stove at 55˚C until constant weight, recording the weight to estimate the dry matter per hectare, at the different phenologies, dividing the leaf between the stem.

Weight Per Stem
The weight per stem was recorded one day before weekly cutting, 10 stems of American Journal of Plant Sciences each grass were cut at ground level, dried in a forced air stove at 55˚C until constant weight and their weight was recorded. The average weight per stem was obtained by dividing the total weight by the number of stems harvested.

Statistic Analysis
The data were analyzed using a completely randomized block design with arrangement in divided plots and four replications, the procedure used was PROC GLM [22], where the cutting frequency effects were considered as fixed. The multiple comparison of means of the treatments was performed using the Tukey test (α = 0.05). On average, all the grasses presented lower stem density at the beginning of the growth curve (day 7), and it can be attributed to the beginning of the growth curve since at the beginning of the regrowth the tillers tend to have a lower population of stems and the largest foliar area and, therefore, the higher the weight with time to establish the meadow [23]. Another factor that affected this behavior of stems was the inter-specific competition for nutrients, water, light and space [24], since the plants do not regrowth in a meadow as isolated individuals, but as a usually dense population, where the vegetation that It surrounds them exerts a very strong influence on the inherent characteristics of each species, through competition [17].

Dynamics of Stems Population
Other authors, [25] in the dry tropics in the population density of stem, ob-   Several authors [21] [28] mention that at the end of the forage accumulation curve senescence losses increase and, therefore, the net forage accumulation decreases as in this investigation. In Cobra grass (Urochloa hybrid) and Cuba 22

Tissue Replacement
(Pennisetum purpureum x Pennisetum glaucum) the maximum leaf growth occurs in the seventh; then growth rates decrease due to a progressive increase in stems and senescent material [16] [27]. The highest growth and trend of these Urochloa grasses is related to a promotion of division and lengthening cell, known effects of cytokinins on cotyledons and leaves of mustard (Brassica nigra) cucurbits (Cucurbita pepo) and sunflower (Helianthus annuus) [29]. After defoliation, the speed with which a forage recovers depends on the reserve carbohydrates in roots, remaining leaf area and the number of vegetative buds started, which increases the photosynthetic capacity of the leaves; and higher elongation and net foliar growth [13]. decreasing on day 49 with 3.8 of leaf:stem relation, trend and results similar to those of this study [30]. Other researchers [15] in mulatto I (Brahiaria hybrid) now Urochloa report similar results to those of this investigation, they mention that the high values of leaf:stem relation were associated with the management given in the meadow, mainly leaving remaining foliar area, which prevented a large amount of stem from being harvested, which is located close to the soil surface. The weight per stem is closely related to the forage yield since as the weight per stem increases, the weight of a tiller and therefore a meadow increases [23].

Weight per Stem
In this regard, the age of the plant is a factor that determines an increase in weight per stem and senescent material and decreases leaf formation [15]. On the other hand, when evaluating Cuba 22 grass in the dry tropics, they reported the same trend and higher weights per stem than those of this investigation; however, they evaluated a species with higher height, yield, therefore, the weight per stem was higher [27].

Conclusion
As the regrowth age increased, the population dynamics of stems and weight per stem increased; in Insurgent and Signal the leaf elongation and net growth increased to a maximum point to start to decline; however, in Piata grass was increasing without decreasing. The trend of the leaf:stem relation was decreasing as the test progressed and senescence increased from day 21 of regrowth. Longer evaluation time is recommended and at different times of the year to expand the M. Á. Maldonado Peralta et al.