Possible Mechanical Transmission of SARS-CoV-2 Causing COVID-19 by Insects: Infection, Prevention, Implications, and Control

The new coronavirus called SARS-CoV-2 is a new type of virus named as COVID-19. Although, it has few similarities with pandemic flu viruses, the respiratory system and immune system are damaged through the viruses infected the population who has weakened immunity. SARS-CoV-2 spreads when people don’t have the sign and symptoms. This virus COVID-19 appears to spread more easily than the flu, and asymptomatic transmission may account for a greater proportion of COVID-19’s spreader over the World. In inundation of the current understanding, the roles of insect vectors are helping in the transmission of viral pathogens as well and the possible roles of some newly joined insects in the mechanical transmission of COVID-19. We also specifically provide the prevention and control methods related to contamination, disease burden, risk pattern in the family, near and dear to maintain the precision of social distancing and development of the immune system to fight against SARS-CoV-2.

global pandemic [7]. The COVID-19 pandemic may represent the most significant public health emergency that the world has faced in a century.
Health care workers (HCWs) and nonetheless, coronaviruses have been implicated with reports of transmission related to environmental contamination [8]. Experimental evidence also suggests that SARS-COV-2 can be transmitted by aerosol but the role of insect in mechanical transmission and environmental contamination remains unclear [9] although many groups of insects are always in close contact with the patients as well as the environment in which these patients are isolated. The present study aimed to investigate the possibility of COVID-19 transmission by insects. Arboviral diseases (arthropod-borne viral) are caused by a wide variety of RNA viruses with a life cycle that requires both a host (birds or mammals) and a vector [10]. Human ecology and behavior are two factors that play a key role in the emergence or re-emergence of these infections [11]. There are at least 135 arboviruses that have been recognized to create human disease (Dash, Bhatia, Sunyoto, & Mourya, 2013). Arboviral infections can range from asymptomatic to fatal disease. The clinical symptoms are commonly grouped as invasive neurological diseases, hemorrhagic fever, and systemic febrile illness [12]. Re-emerging and emerging pathogenic agents of zoonotic origin have become an important universal fear [13]. Arboviruses are a group of viruses that replicate in both their arthropod and vertebrate hosts. They are transmitted between vertebrates through the bite of blood-sucking arthropods. The vast majority of arboviruses belong to one of the five family's Togaviridae, Flaviviridae, Bunyaviridae, Reoviridae, and Rhabdoviridae [14]. We are trying to implement an effective public health measure (EPHMs) for understanding the modes of transmission of emerging infectious disease is a key factor. Due to the lack of the maximum evidence on SARS-CoV-2 transmission dynamics misleads to shift in isolation guidelines between airborne and droplet isolation precautions [15]. WHO, US CDC, and other public health authorities. Other emerging coronaviruses (e.g. SARS and MERS) have been suggested to have airborne transmission potential [16] in addition to more direct contact, and droplet transmission would be known better in this research article example Open Journal of Medical Microbiology with and evidenced-based.
Although, the origination of this disease is still unknown, very recently its outbreak located in Wuhan, China, in late 2019 with the high epidemic ability and human mortality rate (HMR) are higher. Now, it is a pandemic and affected more than 190 countries. It has been suggested that the COVID-19 virus transmission and evolution path occurred from bats to scaly anteaters (pangolins) and then to the human groups [5].
In general, respiratory virus infection can occur through contact (direct or indirect), droplet spray in short-range transmission, aerosol in long-range transmission (airborne transmission) [20] (Table 1). As with other respiratory pathogens, including flu and rhinovirus, the transmission is believed to occur through respiratory droplets from coughing and sneezing. Aerosol transmission is also possible in case of protracted exposure to elevated aerosol concentrations in closed spaces. Analysis of data related to the spread of SARS-CoV-2 seems to indicate that closer contact between the individuals is necessary. COVID-19 is a new disease and we are still learning about how it spreads according to the US Centers for Disease Control and Prevention [25]. The virus is thought to spread mainly between people via respiratory droplets from an infected person's coughs and sneezes within about 6 feet. Despite that, some experts think that 6 feet (1.8 meters) are not enough [26]. Air Currents influence the movement of respiratory droplets. Larger respiratory droplets (>5 μm) remain in the air for only a short time and travel only short distances, generally <1 m (less than 3.3 feet) [27] [28] [29]. Virus-laden small (<5 μm) aerosolized droplets can remain in the air and travel long distances, >1 m (more than 3.3 feet) [30].
Temperature and humidity modulate the viability of viruses by affecting the properties of viral surface proteins and lipid membranes [20]. Relative humidity (RH, or Saturation Ratio: the state of vapor equilibrium in room air) affects all infectious droplets with respiratory viruses, independent of their source (respiratory tract or aerosolized from any fluid) and location (in the air or settled on surfaces). Relative humidity, therefore, affects all transmission ways but has the most pronounced effect on airborne transmission. Transmission of these viruses takes place throughout the year Measurements of indoor humidity showed indoor vapor pressure of below 10 Mb or indoor RH of below 24% in the winter.
Hence, these show that the Transmission of these viruses takes place throughout the year in all types of weather conditions. 13.1 feet is seen as the maximum transmission distance of SARS-CoV-2 aerosol, in hospital settings, as such medical staff and other close contacts are at high risk for infection [31] ( Figure 1). This is true in a hospital setting and doesn't necessarily apply to other settings.
In the context of COVID-19, when procedures or support treatments that generate aerosols are performed there may be possible airborne transmission; i.e., endotracheal intubation, bronchoscopy, open suctioning, administration of nebulized treatment, manual ventilation before intubation, turning the patient to the prone position, disconnecting the patient from the ventilator, non-invasive positive-pressure ventilation, tracheostomy, and cardiopulmonary resuscitation [23]. Information on the transmission reveled that, SARS-CoV-2 remained viable in aerosols for 3 hours on several environmental conditions (aerosols, plastic, stainless steel, copper, and cardboard) [24] despite the WHO notes that these findings need to be interpreted carefully in this experimental study because aerosols were generated using controlled laboratory. Further, the finding of COVID-19 virus in aerosol particles up to 3 hours does not reflect a clinical setting in which aerosol-generating procedures are performed-that is, this was an experimentally induced aerosol-generating procedure [23]. Open Journal of Medical Microbiology The virus could spread by touching an object or surface with a virus present from an infected person, and then touching the mouth, nose, or eyes [32]. 76.5% of all personal items sampled at the University of Nebraska Medical Center (UNMC) were determined to be positive for SARS-CoV-2 [7]. Although the virus titer was greatly reduced, viable SARS-CoV-2 was measured to last on plastic up to 2 -3 days, stainless steel up to 2-3 days, cardboard up to 1 day, and copper up to 4 hours. There is a high positivity for floor swab samples, this might be due to gravity and airflow causing most virus droplets to float to the ground. There is also a 50% rate of positivity from the soles of the ICU medical staff shoes [32].

Insects as Vehicles for Mechanical Transmission of Virus
Arthropods are divided into class namely; insects, crustaceans, arachnids, and myriapods [32]. Insects consist of the most abundant known species of animals of all kinds which can exist as parasitic or freely living forms in nature. Parasitic arthropods often act as a vector in disease transmission by mainly feeding on the blood of animals and humans [33]. However, just because an arthropod feeds on a diseased host does not ensure that it can become infected, nor does it ensure that ingested pathogens can survive and develop. These arthropods transmit pathogens via the two methods.
Mechanical transmission is also called as the passive transmission. It involves the use of contaminated mouth-parts, legs, setae, or any other body parts of the arthropods in the transfer of the pathogens with no developmental change or multiplication of the pathogen on or in the arthropod. Hence, they only act as a transfer medium [21]. Cockroaches also act as the same to disease in their mouth, legs. Likewise, housefly can cause diarrhea and dysentery by transferring bacteria from infected human feces and contaminate food (Figure 1).
The active transmission is also called as the biological transmission. It is the

Transmission Process of COVID-19 by Insects
The Coronavirus Disease initiates or appeared in the animal world before hitting humanity. These viruses can be transmitted by direct contact with an infected animal or by consuming meat or other products that did not pass through the appropriate processing. As for the insects, there is still no proof that they can or cannot carry the virus. This means that insects are potential virus carriers, and we should beware of them until scientists figure out the truth [34].  them travel very far and get into any place. They can infect domestic animals and people with oodles of pathogens, including helminthiasis, encephalitis, and many others. Of course, the middle ages are history, but there are no guarantees that fleas that feed on bats are not COVID-19 carriers.
On the other hand, cockroaches being nasty and nimble bugs are some of the most dangerous insects. They breed incredibly fast and eat any garbage that appears on their way. They are among the most notorious pests of premises, which not only contaminate food by leaving droppings and bacteria that can cause food poisoning but also they transmit bacteria, virus, fungi and other pathogenic microorganisms in infested areas [36]. Their nocturnal and filthy habits make them ideal carriers of various pathogenic microorganisms. They are tropical insects, so far numerous pathogenic bacteria, including Salmonella spp., Shigella spp., and K. pneumonia have been isolated from cockroaches. Besides, some parasites and fungi have been found in external surfaces or internal parts of the body of cockroaches [37]. Mosquitoes are famous carriers of encephalitis, malaria, and several others that are jeopardous as well as hardly treatable diseases. Every year, they infect over 200 million people in Asia, the Middle East, South America, and Africa. They can carry infected blood within their paunches and infect several people during their short lives.
There have been no reports of COVID-19 transmission by blood-sucking arthropods such as mosquitoes and lice [38]. However, major mechanical vectors of pathogens such as cockroaches and houseflies may be able to transmit the disease by contact with contaminated surfaces and even with the feces of infected individuals. In a limited number of case reports, the live virus has been reported in the feces of patients [23] [39] [40]. SARS-CoV-2, the virus that causes COVID-19, can be detected in the stool of up to 53.4% of COVID-19 patients [41] [42] [43]. Furthermore, despite the stool samples were still positive, about 23% of patients were no longer positive for the virus in respiratory samples [44]. Data from 98 COVID-19 patients showed viral shedding in the stool for nearly five weeks after respiratory samples were negative [45]. Another study reported that 30 days after initial infection, patients still shed the virus in the stool [30]. Even asymptomatic carriers may show elevated SARS-CoV-2 in the stool [42].
Although knowledge about the viability of SARS-CoV-2 is limited, the virus could remain viable in the environment for days, which could lead to fecal-oral transmission [46]. In a study to investigate SARS coronavirus carried by cock-

Prevention of Transmission and Control
SARS, MERS, and now COVID-19 (coronavirus disease 2019) are zoonotic coronaviruses that have jumped the species barrier from their normal animal hosts to humans. This can happen through direct contact with an infected animal, as well as indirect contact or eating contaminated food. Zoonotic diseases are also very common and once transmitted to humans, they can spread through person-to-person contact as well, making proper handwashing and other preventative actions outlined by the CDC paramount to public safety [48]. SARS-CoV-2 infection may occur in both direct and indirect contact during respiration, toileting, and fomite contact [7].
WHO protocols for Covid-19 do not involve the possible transmission of the virus by insects that have close contact with the patients as well as the healthcare workers. There have been reports of COVID-19 transmission in prisons, hospitals, and other places where people live together for a long time from various Chinese provinces [23]. The proximity and close contact of people in such places and the potential for environmental contamination are important factors that can enhance transmission [23] [24]. Dealing with the COVID-19 virus and preventing its rapid and dangerous spread is a worldwide challenge that requires universal management.
This management involves personal hygiene, biological, physical, and less risk of chemical control. In this regard, maintaining appropriate environmental hygiene is of great importance especially bathrooms, toilets, and kitchens which has the highest infestation of cockroach and houseflies in the household environment [49]. It is also important to eliminate possible mechanical vectors such as cockroaches and flies in public places and residential homes. Control methods of these vectors include improved environmental sanitation. Installing stainless or plastic screens on doors, windows, and ventilators, covering food containers, and using poisoned baits, light traps, and sticky traps are some physical and mechanical control methods of these insects. Besides, medicinal plants such as Veratrum nigrum and eucalyptus are also very effective in repelling houseflies, mosquitoes, and cockroaches [40] [50] [51]. Biological control and application of parasitoids is a safe and environmentally friendly way of controlling these insects. Decaying materials, landfills, the areas around livestock and poultry farms, animal carcasses, garbage cans, and so on can attract houseflies and cockroaches to their lay eggs on them. Isolation centers should employ the use of nets to reduce possible contact with insects that might come in contact with respiratory droplets on their body or within their vicinity.

Conclusion
Coronavirus infection is a growing concern globally. Transmission management is now the primary option to prevent and control outbreaks of the disease in the absence of a vaccine. Most of the information generated on the possible transmission of COVID-19 is based on the already infected cases that are recorded.
But enough efforts are not made to prevent and control possible transmission from other sources. Several kinds of research have shown the viability of SARS-CoV-2 in human pieces which have a longer duration than in respiratory droplets. The virus is also having a larger size compared to other families of coronavirus. This indicates that there is a possibility of transmission by an insect that can contact with patients or their droplets and feces. Several managements such as personal hygiene, biological, physical, and less risk chemical control should be employed.