A Comparative Study of Fungal and Some Aflatoxin Contents of Freshly Fried and Stored Sallah Meat from Danbatta Local Government Area of Kano State, Nigeria

A comparative study of Fungi (yeast and moulds) and their toxic metabolites was carried out on offal and muscles of freshly fried and stored Sallah meats from rams, bulls, goats and camels slaughtered for sacrifice in Danbatta Local Government Area, Kano State, Nigeria. The objective was to determine the effects of frying and four weeks storage at ambient temperatures on the mycological loads of the fried and stored meats. A total of seventy two (72) samples of muscles and offal were collected from six different and randomly selected households that slaughtered and fried any of the four animals during that period. The samples were analyzed immediately as freshly fried, and then stored for a period of four weeks during which analyses were conducted at the second and fourth weeks. An analysis of variance (ANOVA) was used to compare between the fungal loads and the toxin contents in the stored meat. Results revealed the presence of Mucor, Rhizopus stolonifer, Aspergillus niger, muscles and offal of the slaughtered animals; and the toxins were far below the lethal doses recorded in literature. It was concluded that, the fungal organisms were detected in the fried Sallah meats, but not in significant numbers as to cause health risks to the consumers within the short storage time.


Introduction
The term Sallah meat is popularly known as Naman Sallah among the people of Northern parts of Nigeria. It is meat obtained from the scarified slaughter animals during one of the highly celebrated Islamic Festivals (Eidul Kabir); which is a period in Islam that falls in Islamic month of Zul-hijjah (the twelfth month in Islamic Calendar). The animals recommended for the sacrifice must be at least one year old and of different Halal (i.e., not prohibited for consumption) species including cows, bulls, rams, goats sheep, and camels; and must be free from the 4Ds (diseased, downed, disabled, or dead). The sacrifice must be performed depending, on an individual's option that best suits him or her, between the stipulated dates, that is, on the 10 th , 11 th and 12 th days of the month of Zul-hijjah.
Meat is defined as the edible part of the skeletal muscle of an animal that is healthy at the time of slaughter. It is composed chemically of four major components including water, protein, lipid, carbohydrate and many other minor components such as vitamins, enzymes, pigments and flavour compounds [1]- [9]. The relative proportions of all these constituents give meat its particular structure, texture, flavour, colour and nutritive value. However, because of its unique biological and chemical nature, meat undergoes progressive deterioration from the time of slaughter until consumption [10] [11] [12] [13] [14].
In many developing countries, including Nigeria, meat is widely consumed as a source of protein; it is either eaten cooked or processed into other forms to avoid any form of spoilage [15] [16] [17] [18] [19]. The rapid expansion in meat processing plants in different parts of the world is due to continuous increase in demand for meat products that are highly nutritive, safe and most importantly, affordable [20] [21] [22]. Meat processing, also known as further processing of meat, is the manufacture of meat products from muscle meat, animal fat and certain non-meat additives. Additives are sometimes used to enhance product flavour and appearance [23]. They can also be used to increase product volume. For specific meat preparations, animal by-products such as internal organs, skin or blood, are also well suited for meat processing. Meat processing can create different types of product composition that maximizes the use of edible livestock parts and is tasty, attractive and nourishing [3] [11].
Many foodborne diseases have been associated with consumption of meat and F. S. Abdullahi et al. its products. Some of the meat carcasses on sale might be contaminated with one pathogen or another and this has been reported to be very common in developing countries [8] [24] [25]. The pathogens of concern in fresh and frozen meat and meat products include, in addition to bacteria, the genera from yeast and moulds that produce myco-toxins in the ready-to-eat products [26] [27].
Myco-toxins are low molecular weight metabolites which cause the harm known as mycotoxicosis, in livestock, domestic animals and humans and therefore are of public health significance [28] [29]. The production of myco-toxins is stimulated by certain environmental factors. Therefore the extent of contamination will differ with geographic location, agricultural methods and the susceptibility of commodities to the penetration of fungi particularly during storage periods [30]. Fungi that produce toxins in food are classified into field and storage fungi based on their ecological requirements for growth [

Sample Collection
Danbatta LGA in Kano State was chosen for this study, because of the habits of the inhabitants of storing the fried Sallah meat for a long period after the Sallah celebrations. Samples (200 g each) of freshly fried Sallah meats were collected directly from randomly selected households in the area at the evening hours (4 -7 pm) of the 10 th to 12 th of the Holy Month of Zul-Hijjah, in previously sterilized containers, and then transported to the laboratories for the mycological assessments. The samples used in the assessments were the edible parts consisting of the muscles and offal from the slaughtered (scarified) rams, bulls, goats and camels. A total of 72 samples were collected from six different households for each type of the four sacrificial animals and the analyses were conducted in triplicates.
The analyses were carried out at two weeks interval, starting from zero to the fourth week which is the maximum period the Sallah meat is usually stored by the inhabitants at ambient temperatures.

Sample Preparation
The meat samples were thoroughly grind using previously sterilized pestles and mortars and 11 g were transferred into 99 mL of sterile peptone water, and then shaken thoroughly to make a homogenous mixture (these served as stock solutions for each sample). Serial dilutions were made using 1 ml from the stock homogenate and 9 ml of sterile distilled water. Several dilutions were made, up to 5 folds (i.e., 10 5 ) for each sample, in order to obtain discrete colonies as described in [38] [39].

Preparation of Media
The following media were used in the mycological assessment: potato dextrose agar (PDA) and eosine methylene blue agar (EMBA) for mold and yeast counts, respectively. These media were used for the enumeration of the fungi as well as in the pure culture selection of the organisms; each medium was prepared as instructed by their manufacturers. All glassware, including Petri-dish, test tubes, pipettes, flasks and bottles used in the analysis were sterilized in a hot oven at 170˚C ± 5˚C for at least two hours, while the media and distilled water were sterilized by autoclaving at 121˚C for 15 min, at 15 psi [9] [39]. Plating was carried out in triplicate and pour plate method was used to make the viable counts [40] [41] [42]. In this method, one (1) ml of the inoculums was mixed thoroughly in molten plate count agar held in a hot water bath at 47˚C ± 2˚C. The agar was allowed to set; the plates were inverted and then incubated at 25˚C for 5 -7 days for yeast and mold counts [43]. For each dilution, the viable colonies, which appeared colorless, in the three plates were counted and the means were calculated.

Characterization of the Fungal Isolates
The fungi were identified using lactophenol cotton blue technique. In this process, a drop of lactophenol cotton blue was placed on the grease free slide. A straight wire loop was used to pick the organism from the colony and placed on the drop. A cover slip was placed on the lactophenol cotton blue and examined under a microscope (X4 objective lens) to check for the morphological characteristic of the organisms [43].

Mycological Analysis Using a High Performance Liquid Chromatography (HPLC)
was added to 10 g of the sample and then homogenized for 3 min with a homogenizer, poured into a 100 mL centrifuge tube, the homogenate was centrifuged at 10,000 rpm for 10 min at 4˚C; 16 mL of the supernatant was evaporated to dryness under a stream of Nitrogen at 50˚C. The residue was re-dissolved using 8 mL of a mixture of ethylacetate/cyclohaxane (50:50 v/v), filtered with 0.45 μm nylon filter paper, the filtrate was again dried using a rotary evaporator and the residue was then re-dissolved in 1 mL of methanol/10 mMol/L ammonium acetate (1:1 v/v). Finally, the solution was passed through 0.22 μm nylon filter paper prior to injection into the HPLC [44]. The excitation and emission wavelengths for the aflatoxin detection were set at 360 nm and 450 nm, respectively.

Statistical Analysis
This study was designed as a complete randomized design (CRD) and the results obtained were subjected to a one way analysis of variance (ANOVA). The test for significance among means was carried out using the Tukey's Honest Significant Difference (HSD) Test at 5% level of significance [45].

Results and Discussions
The muscles of healthy animals are usually regarded as sterile, but the slaughtering and butchering processes can provide microorganisms with an opportunity to colonize the meat surfaces. Contamination of meat is a continuing possibility from the moment of exsanguinations of the meat animals until consumption. In the abattoir itself there are many potential sources of contamination of meat by microorganisms. These include the animal hide and hair, soil adhering thereto, the contents of the gastrointestinal tract (especially if inadvertently released during dressing operations), airborne contamination, aqueous sources (the water used for washing the carcass, or for cleaning the floors and equipment), the instruments used in dressing (knives, saws, cleavers and hooks), various vessels and receptacles, and the personnel [9] [39] [42] [46]. The aerosols produced during de-hiding, evisceration, and carcass splitting are also important sources of contamination [20]. Air circulated from heavily contaminated refrigeration coils in meat and poultry processing plants can also serve as potential sources of contamination. The initial microbial load of a carcass surface is determined by the hygiene observed during the slaughtering operations as well as handling practices [47]. The observations recorded in the present study are presented in Tables 1-10 with some of their statistical interpretations. To start with, the total fungal counts, expressed in colony-forming unit (cfu × 10 3 ) per gram of different parts of the fried Sallah meat, recorded in this study are presented in Table 1, with their standard deviations. Statistically, significant differences (P ≤ 0.05) were observed between the fungal contents of the freshly fried meat parts (muscles and offal) in the zero weeks and that of the second and fourth weeks of the storage periods. The initial fungal loads were generally found to be less in the freshly fried meats when compared with the stored ones, though in most cases, no significant differences (P ≥ 0.05) were observed between the fungal loads of the meat parts during the second and fourth weeks of the storage (Table 1). In overall, the lowest fungal counts, 0.27 ± 0.06, was recorded from the muscles of camels at zero week, whereas the highest, 6.37 ± 2.11, was from the goats' offal during the fourth week of the storage period. The gradual increase in fungal counts as the storage period progressed may be as result of surface contaminants or due to the gradual lost in water activity (a w ) which makes the fried meat environment more conducive for the growth of yeast and moulds. Foods are described Results of Isolation and Identification of the Fungal Contents of the Sallah Meat: The control of water activity in foods is an important tool for extending shelf life of food products. It is responsible for the quality of foods affected by microbiological, chemical, and physical changes. It has been proven that physical properties, quantity, and quality of water within the food have a strong impact on food effectiveness, quality attributes, shelf life, textural properties, and processing [13] [50]. Food preservation processes have a common goal of extending the shelf life of foods to allow for storage and convenient distribution.
The activity of microorganisms is the first and most dangerous limitation of shelf life. Water is essential for microorganisms that may cause food spoilage if they are present in a food that offers them favorable conditions for growth.
Hence, many food preservation techniques were developed to reduce the availability or activity of water in order to eliminate the dangers of microbial spoilage.  (Table 6 and Table 9), whereas the highest frequency of the fungi recorded in the muscles were 40.84% and 51.88% for goats and bulls, respectively ( Table 7 and Table 8); and Saccharomycete was the least in the frequency of occurrence among all the fungi isolated, especially from the second to          [21] which stated that the quality of meat and meat products produced by processors varies from one processor to another due to lack of standard methods of preparation that would ensure consistent product quality.

Results of Quantification of the Mycotoxins Contents of the Sallah Meat with HPLC:
The best approach to eliminating mycotoxins from food is to prevent fungal growth at all levels of production, harvesting, transporting, processing, storage, and marketing. Among all the mycotoxins, aflatoxin is considered to pose the greatest potential hazard to human health. It is produced by A. flavus and A. parasiticus, which are nearly ubiquitous with spores that can widely be disseminated by air currents. Among these two fungi, only A. flavus was identified and isolated in this study and its frequency of occurrence was not significant, especially between the second and fourth weeks of the storage periods. However, the aflatoxin contents of the fried and stored Sallah meats as determined by HPLC and expressed in parts per billion (ppb), are presented in Table 10. The four types of aflatoxins, B 1 , B 2 , G 1 and G 2 , were not detected in the majority of the meat parts analyzed. Moreover, the highest levels recorded were 7.73 ± 0.72, 5.35 ± 0.85 and 5.10 ± 0.72 ppb of B 1 , B 2 , and G 2 , respectively, obtained from the muscles of rams, camels and goats (Table 10). These values are still far below the Lethal Dosage (LD 50 ) recorded in literatures in most animal experiments, which ranged from 0.5 to 10 mg/kg (ppm) body weight [27] [30] [31] [32] [36]. The complete absence or low levels of aflatoxins recorded in this study is in correlation with the fact that the majority of fungi isolated from the fried and stored Sallah meats were not of the types known to produce aflatoxins in foods, which   [36]. The environmental conditions that influence toxin production most, appear to be temperature and water activity (a w ); with the optimal temperature for toxin production being 24˚C -28˚C and that of a w is 0.93 -0.98 [27]. Control of mycotoxin production in foods is complex and difficult. There is insufficient information regarding toxicity, carcinogenicity, and teratogenicity to humans, stability of mycotoxins in foods, and extent of contamination. Such knowledge is required to establish guidelines and tolerance levels in food. Moreover, ingestion of large doses of aflatoxins have been reported to be acutely toxic, causing gross liver damage with intestinal and peritoneal hemorrhaging, resulting in death [22] [37]. Toxicity of aflatoxins have also been reported in decreasing order as B 1 > G 1 > B 2 , > G 2 .
Other foods in which aflatoxins may be produced include: peanuts, peanut butter, nuts, fresh beef, ham, bacon, milk, cheese (through contaminated feed to dairy cattle), beer, cocoa, raisins, soybean meal, corn, rice, wheat and cottonseed [26] [30] [32] [48] [52]. Finally, microbial food spoilage is a complex process. Fungal growth to high numbers is considered a prerequisite for the development of mycotoxins in many different types of food products, including meats. The sensory signs of fungal spoilage are many and varied; they may be visual (slime, swelling, discoloration), or be apparent by smell or taste (off-odours and off-flavours). These signs are typically a result of the accumulating by-and end-products formed during metabolic activity of the organisms, and none of these signs or spoilage indices were detected from any of the fried stored Sallah meats at the end of the four weeks storage period; indicating high level of hygiene in the preparation of the fried meats.

Conclusion
Among the desirable qualities that should be associated with foods is freedom from infectious organisms. Although it may not be possible to achieve a zero tolerance for all such organisms under good preparation practices, the production of foods with the lowest possible numbers should be a desirable goal. Harmful microbes may enter the manufacturing process and reach the end product in several ways: through raw materials, air in the preparation environment, ingredients employed, contact surfaces, or the handlers. There is high incidence of cancer among the inhabitants of Danbatta LGA in Kano; one of the major suspects of the cancer incidents in the area is the possible growth and toxin production in the fried Sallah meats consumed after an extended period of storage. This study has, however, confirmed the presence of fungal organisms in the fried Sallah meats, though not in significant numbers as to cause health risks to the consumers within the short storage time. The study concludes that the levels of aflatoxin B 1 , B 2 , G 1 and G 2 detected were far below the minimum lethal dosage and may not be associated with the high incidence of cancer in the area. The study however recommends that more hygienic processes be observed during Sallah meat preparations and handling, and that the meat should not be stored at ambient temperatures for more than four weeks.