Total Trihalomethane Levels in Major Watersheds across the United States

Trihalomethanes, such as chloroform, bromoform, dibromo(chloro)methane, and bromo(dichloro)methane, are present in the major watersheds across the United States. These chemicals play an important role in the development of cancer, have adverse reproductive outcomes, and were found to be present above the threshold established by the Environmental Protection Agency. To understand the impact of socioeconomic background on the quality of water and possible disparities, we have analyzed the levels of total trihalomethanes in the metropolitan areas in the major watersheds across the United States, in 2018, as they correlated to average household incomes. Our study found that Arkansas, Nevada, and Rhode Island demonstrated higher than federally mandated levels of total trihalomethanes in their watersheds. The median annual household and per capita incomes of the three states (Arkansas, Nevada, and Rhode Island) were lower than the national average. In addition, Delaware, New Hampshire, and Wisconsin, which had higher median income levels, demonstrated the lowest total trihalomethane levels across the United States.


Introduction
Drinking water is often derived from groundwater or surface water. However, these water bodies all drain into drainage basins along with rain runoff and melted snow (The Physical Environment, 2003). A watershed is a topographic region that drains into a river, lake, or stream (What Is a Watershed and Why Journal of Geoscience and Environment Protection Should I Care?, 2012). Watersheds can essentially act as a funnel for collecting water from surrounding rivers, lakes, bays, sub-watersheds or any other water bodies, and drain them into a larger water basin. Considering the flow of water, it is empirical to examine the composition of water bodies as they integrate and play a significant role in the distribution of contaminants.
Pollutants have contributed to the increase in the acidity of raw water (Brewer & Barry, 2008), which can significantly impact the integrity of water quality.
Total organic halides, present in raw water, are the main precursors for the formation of trihalomethanes (Jung & Son, 2008). Hence, watersheds containing large volumes of total organic compounds are more susceptible to the presence of higher levels of trihalomethanes (Babcock & Singer, 1979). The presence of humic acid is shown to contribute to very high levels of bromoform formation during the ozonation process of water disinfection (Huang et al., 2005). According to the Environmental Protection Agency (EPA), chlorinated water contains varying trihalomethane levels (Symons, 1975), and concentration of these carcinogens may vary depending on water composition (Guha et al. 2019). In addition to water acidity or alkalinity, algal abundance can also contribute to the elevation of organic precursors that can further react with chlorine residues to form trihalomethanes (Saunders et al., 2015).
Epidemiological studies have attributed various reproductive health anomalies to the consumption of water containing trihalomethanes and deemed it as a carcinogenic chemical (Symons, 1975). Scientists have demonstrated a positive correlation between trihalomethane oral consumption and an array of sexual abnormalities in rats including toxicity to fetus (Thompson et al., 1974), embryonic death and anomalies in the sperm formation (Klinefelter et al., 1995). Human research has been limited; however, a handful of investigators have determined similar reproductive anomalies in human subjects (Waller et al., 1998;Grazuleviciene et al., 2011). Exposure to trihalomethanes can slightly increase one's susceptibility to colorectal and bladder cancers (Villanueva et al., 2017;Klaassen, 2019), and cause major organ damage (World Health Organization, 2004 (Freyfogle, 1986). Studies have shown that overwhelming amount to toxic chemical as a result of industrial use is released in locations populated with low income communities calling this practice "environmental racism" (Collins et al., 2016). In addition, infrastructure for the treatment and disposal of toxic waste tends to also be built in low-income communities (Mohai & Saha, 2015).
In Tennessee, lower income communities were shown to have higher levels of trihalomethane presence albeit being below federally mandated regulations (Guha et al., 2019).
In order to address the disparities in attaining justified distribution of water, investigators have formulated framework studies to elaborate on the existing environmental inequalities, attributing them to sociocultural factors, and have attempted to offer possible remedies (Zwarteveen & Boelens, 2014). To our knowledge, there have not been any studies conducted to explore the relation between the levels of trihalomethanes and median household incomes in the watersheds affecting metropolitan cities across the United States. Our research is the first of its kind to explore trihalomethane concentrations in the most densely populated metropolitan city in each state, during 2018, in an attempt to evaluate watershed conditions across the country. In this manner, our research provides novelty and adds to the existing literature on contaminants affecting water quality in different areas of the United States. The data obtained will be used to analyze watershed health and uncover any disparities that may be present in the levels of total trihalomethanes between metropolitan areas with different median household incomes. The information provided by our research will also be useful in educating and empowering people from low-income communities, and with enable them to take appropriate measures about the water quality affecting their areas.

Materials and Methods
Secondary data related to the concentrations of trihalomethanes was obtained from the annual water safety report for the metropolitan area watersheds across lected by contacting water service offices to obtain information not readily available in the annual water safety report. The molecular structures of trihalomethanes are shown in Figure 1 (Jung & Son, 2008;Singer & Chang, 1989;Bellar & Lichtenberg, 1974;Rook, 1974).

Results and Discussion
The water chemistry and composition of raw water can be influenced by many factors including human activity and wildlife population surrounding the water source. The water source or watersheds can be comprised of different types of water bodies including aquifers, lakes, rivers, creeks and reservoirs. The concentration of fulvic and humic acids present in these water bodies highly influences the level of total trihalomethanes present due to chlorination of potable water. Regardless of water source or raw water composition, trihalomethanes levels must not exceed federally mandated levels. Each state must follow federal and state laws to ensure the availability of clean, regulated water to all citizens, regardless of socioeconomic background or location. Table 1 shows the watersheds or water source in the metropolitan city of each state in the United States. As shown in Figure 2, three states (Rhode Island, Arkansas and Nevada) demonstrated the highest levels of trihalomethanes across the nation. Water sources or watersheds for these states included lakes, creeks and reservoirs indicating that the types of water source did not play a significant role in the level of total trihalomethanes.
Although the EPA sets the standard for maximum allowable level for total trihalomethanes at 80 ppb, the data for the metropolitan cities of Rhode Island, Arkansas and Nevada proved to be in violation of the federally mandated regulations.
When examining the median annual household income and income per capita (Table 2) for these three states in violation, it was determined that two (Arkansas and Nevada) out of the three states had income that was significantly lower than the national average household income of $60,330, as provided by the United States Census Bureau. In addition, three states (Delaware, New Hampshire, and Maryland) with the lowest levels of total trihalomethanes belonged to higher income groups, as they had median annual household income and income per capita above or at the national average (Figure 3).
These disparities in total trihalomethane levels in correlation to income levels are in conjunction with water justice inequalities that have been previously investigated by scientists and environmentalists. Our findings that lower income communities face injustice obtaining and sustaining high water quality agree with previous investigations (Collins et al., 2016;Mohai & Saha, 2015;Guha et al., 2019). In order to overcome such disparities among households in obtaining and sustaining clean drinking water, the water quality information should be shared with the public, so that they can be better informed. A concerted effort must be made by federal agencies, such as the Environmental Protection Agency, Department of Agriculture, and the Army Corps of Engineers, to collaborate with community-based organizations and bridge water-based community needs. Various steps have been suggested (Vanderwarker, 2013), such as priorities being set by governmental agencies to create specific action plans with time frames for communities facing inequalities in water quality, collaboration of federal agencies with state and local governments, and other stakeholders, to address water-related environmental justice issues, incorporating social, economic, and community health factors into the decision-making processes, and taking proactive steps to ensure purposeful public participation, such as providing information in languages apart from English. Additionally, the methods of data collection and assessment of threats within aquifers and groundwater systems should be improved, and sufficient funding should be allocated to ensure program maintenance. The rapidly growing risks of climate change, such as flooding and    rise of sea levels, should also be taken into account, especially for vulnerable populations, in developing water resource management policies. State and local agencies receiving federal funding should be required to demonstrate collaboration with affected communities, and ongoing efforts to address water quality disparities should be proven, in order to receive continued funding.
Our study could serve as a guide for lawmakers to apportion resources toward the improvement of infrastructure in communities with poor water quality, and to support ordinances issued by the Environmental Protection Agency and other governmental agencies. The results obtained from this research can be used to educate and empower communities, particularly low-income populations, to take action regarding water quality in their respective areas. Our project will provide information about the health risks associated with the presence of trihalomethanes in watersheds, and assist in further data collection and analysis of information. Such steps are essential in order to minimize, and eventually eliminate, the gap between socioeconomic backgrounds and the availability of clean, sustainable water to the population.

Conclusion
Investigating the presence of harmful and possibly carcinogenic compounds in Island, which had median annual household income and income per capita lower than the national average, demonstrated higher than federally mandated levels of total trihalomethanes in their watersheds. On the other hand, the states with significantly low levels of total trihalomethane (Delaware, New Hampshire, and Wisconsin) had high average household income and income per capita. Although many factors play a role in water quality, socioeconomic background should not be one. However, socioeconomic background seems to exasperate poor water quality, particularly in older and less fortunate communities. Additionally, knowing that water bodies are interconnected further increases the urgency to regulate the trihalomethanes entering the watersheds. Information obtained from the present study could be used by governmental agencies to draft guidelines, in order to keep the population better informed of the drinking water quality in their areas. Such information will serve as a precursor in initiating appropriate measures to overcome the discrepancy in the quality of water available to people from different socioeconomic backgrounds.