Gastro Intestinal Stromal Tumor: State of the Art through Our Experience of 64 Cases and a Literature Review

Gastrointestinal stromal tumors are rare. They are a subject of controversy. We have reported 64 cases of gastrointestinal stromal tumor diagnosed in the surgery department of CHU Hassan II of FES between January 2014 and December 2018. The study involved 64 patients (34 men and 30 women) with an average age of 56. The circumstances of findings were dominated by abdominal pain (48 cases), vomiting 16 case followed by transit disorder with 9 cases. The tumor locations were mainly the stomach (n = 31), the small intestine (n = 28), the duodenum (n = 3), and the colon (n = 2). Ultrasound, endoscopy and CT were the main additional tests to detect tumor syndrome. 55 patients were treated by complete surgical excision. Tumor size ranged from 4cm to 18cm. Histologically, the spindle cell type was predominant in 88.91% of cases; epithelioid type was present in 7.81% of cases, while the mixed type was found in 3.6%. The analysis of the expression of CD 117 marker was present in 95.31%, while immunostaining with this marker returned negative in 3 cases; i.e. 5% whose c-kit was positive. Imatinib was indicated in 44 patients (63.60%), with 9 indications for metastatic tumor. As a neoadjuvant, imatinib was indicated in 5 patients, with remission in 28 patients (50.9%), stabilization in 4 patients (7.2%), 2 cases of tumor recurrence and 7 cases of death.


Introduction
typically expressing the KIT + phenotype (95% of case) and DOG-1+ (95% of cases) [1]. Their potential for malignancy is often difficult to assess. Stromal tumors are topical since the discovery of medical treatment of malignant forms with a tyrosine kinase inhibitor molecule (STI571 or GLIVEC). The surgical approach for GIST treatment, combining with molecular treatment makes it a multidisciplinary model of care [2]. We will try through the study of our observations of GIST collected in the department of visceral surgery in Hassan II Hospital in Fez, over a period of 5 years (2014/2018) and a review of the literature to analyze the epidemiological aspects and to study the clinical and paraclinical characteristics as well as their therapeutic and evolutionary modalities to establish the precise diagnosis and to evaluate the therapeutic management of localized, advanced and inoperable tumors, and also to study the place of the surgical act as therapeutic basis of these tumors in the era of medical treatment that is GLIVEC.

Methods
• The type of study This is a descriptive and comparative retrospective study of 64 patients. -Patients over the age of 16.
-Patients with designed histological evidence.  Exclusion criteria: -Any patient presenting outside the period already mentioned.
-Any patient with a histological type tumor other than GIST.
-The study of GIST in children, infants and newborns was excluded.
-Any patient who has not had histological evidence.
-Patients with incomplete records or insufficient data.
-Patients discharged against medical advice.

• Data carriers
The information was collected using an anonymous standardized operating

Result
• Patient characteristics The annual distribution of GIST cases is shown ( Figure 1) with an average of The time between the onset of symptoms and the consultation was between 1 and 24 months. The majority of patients (78.12%) consulted before the first year with a peak frequency of 48.43% between 01 month and 6 months ( Figure 2).
• Clinical and paraclinical presentation The clinical symptomatology was rich. Often each patient had several associated clinical signs ( Figure 3).
The most commun sign was epigastralgia in 48 case i.e. 75% followed by vomiting in 16 cases, i.e. 21.8%, deterioration of general condition and weight loss in 10 patients, i.e. 15.62%, transit disorder in 9 cases, i.e. 14.06% and hemorrhagic syndrome: (6 cases had isolated hematemesis and 2 cases had melena). The gastric localization represented 31 cases among 64% or 48% of the cases, 28 patients had a small bowel localization or 44%, 3 patients had a duodenal localization    In our series, the analysis of the expression of CD117 was made in all cases: 95.31% of the tumors showed a strong expression of CD117, while immunostaining with this marker returned negative in 3 cases, i.e. 5% whose c-kit was positive. In order to assess the degree of malignancy of the stromal tumors in our series, we mainly based on tumor size and location ( Figure 4) (In all patients with resectable tumor) as well as on the mitotic index, i.e. 55 cases (≤5 mitoses in 43 patients and >5 mitoses in 12 patients) ( Figure 5).
• Treatment and evolution In our series we had 55 (85.91%) localized GIST and 9 (14.1%) metastatic.  For localized tumor or 55 cases All 55 patients were operated (Table 1)

Discussion
gastro-intestinal stromal tumor are rare since they represent only 10% of soft tissue sarcomas and less than 1% of gastric tumors, 14% of intestinal tumors and 0.1% of colorectal tumors [3]. The recent recognition of this entity makes it difficult to assess its actual frequency. It is estimated, to date, that GIST affects approximately 15 people in 1 million, which represents approximately 800 to 900  [3], and approximately 10 -15 per million per year in Western countries [4].
In Morocco, a study carried out in Marrakech objectified an incidence of 4.3% of gastric tumors [5]. Our study showed a frequency of 7.6% of gastric cancers therefore a little high compared to that of Marrakech. The incidence of these tumors by age is unimodal, with a peak frequency between 50 and 70 years and an average age of 58 years. The age of our patients varied between 29 and 87 years with an average of 56 years. GISTs are more frequent in men with a sex ratio of 1.5 to 2, as is the case in our series with 34 men and 30 women, with a sex ratio M/F of 1.13. No racial predominance is noted in the literature [3]. Gastric localization is the most frequent (70%), followed by the small intestine (20% -30%) and then come the colon (10%), the rectum and even more rarely the esophagus and mesentery. Among the cases that we have reported Gastric localization represented 31 out of 64 cases or 48% of cases, 28 patients had a small bowel localization or 44%, 3 patients had a duodenal localization i.e. 5% and 2 patients had a colonic localization or 3% [6]. 30% of GIST patients are incidental finding after performing an endoscopy, a sonography for another indication or during surgery for another disease [7]. 70% are symptomatic of which 40% are revealed by a digestive hemorrhage and 10% due to intestinal perforation [8]. In our series pain was the main symptom (75%) followed by vomiting (21.8%), weight loss (15.62%), transit disorder (14.06%) only 12.5% had a hemorrhagic syndrome and no case presented in a table of peritonitis, this can be explained by the precocity of the delay of consultation, the majority of patients had consulted before the first year of their clinical symptoms. In 15% to 25% of cases, the disease is discovered at a metastatic stage [7]. In our series 10.9% had metastatic disease at the time of initial diagnosis. The diagnosis of GIST is based on endoscopic characteristics, echoendoscopic or radiological. However confirmation of the diagnosis of GIST is only histological [1]. The endoscopic appearance of GISTs is not very specific; generally we have a regular nodule, with a submucosal appearance because it is covered with normal mucosa. Endoscopic biopsies are most often negative, endoscopic resection to obtain a histological diagnosis is contraindicated because of the significant risk of perforation [6]. Echo-endoscopy is the best test to characterize oeso-gastro-duodenal or rectal submucosal lesions [3]. The echo-endoscopic appearance of GISTs is often typical: a hypoechoic, oval, homogeneous lesion with regular limits, developing from the fourth hypoechoic layer which corresponds to the muscularis [9]. The CT scan has a preponderant place. It allows to assess tumor extension. Generally, this examination is used to have more precision on the morphological characteristics of the tumor by determining its size, its homogeneity, the presence or not of areas of necrosis or areas of cystization [10]. MRI is an alternative to CT. it is more efficient than the scanner and avoids irradiation, by its multi-planar study capacities, allows a good study of the relationships of the tumor with the adjacent organs [11]. In our study tumors are characterized by the positivity of the CD117 marker in rates between 80% and 95% [12].
Surgery is the only potentially curative treatment for presumed resectable GISTs. The main objective of this process is to guarantee an adequate margin of resection without rupture of the tumor, nevertheless large margins have no advantage, there is no consensus on the distance of necessary safety, which can probably be 1 cm or less when the resection is R0. However, if the tumor adheres to an adjacent structure, en bloc resection taking away the adherent zone is the rule to prevent any tumor intrusion and to ensure complete resection. Lymph node dissection is not necessary due to the rarity of lymph node involvement.
During the surgical procedure, an exploration of the liver and the parietal peritoneum is important in order to objectify possible metastases [3] [5]. On the other hand, imatinib can be indicated after multidisciplinary consultation when it is considered that it can modify the operative gesture by simplifying the surgery or by allowing a less mutilating resection. In our series, the surgery was R0 in 50 patients. Note that, five cases in this group (R0) were initially deemed unrespectable, and who responded favorably to neo-adjuvant treatment based on imatinib received for a period between 3 and 9 months. To evaluate the prognosis a histopronostic classification of GISTs was developed (Table 2) based on tumor size and the mitotic index so a tumor size greater than 2 cm is considered to be a factor of poor prognosis, the threshold of 5 mitoses makes it possible to differentiate tumors from low risk of malignancy [13]. However the threshold of these criteria varies according to the series and there is no international consensus [8]. There is no evidence to suggest that a specific surveillance protocol improves the prognosis. It is based on expert advice and must be adapted to the risk of recurrence, the terrain and whether or not prescription of an adjuvant treatment with imatinib ( Figure 6). Exposure to ionizing radiation and its long-term risks must be taken into account, especially since the patient is young and the  [3]. For locally advanced, inoperable and or metastatic aggressive tumors, the prognosis has improved since the advent of imatinib (Glivec ® ). Its effectiveness is currently established; however the optimal methods of administration are not definitively determined. The daily dose recommended is 400 mg per day, given until the patient's progression, intolerance or refusal (Figure 7) [3] [6].
In our study spread over 5 years has enabled us to determine the different  to the total number and the duration of follow-up which was short over 12 months does not allow us to establish a prognosis. The continuation of our research work could also look into the prognostic aspect of this entity.

Conclusion
GISTs remain rare tumors in adults, mainly found in the stomach and small intestine, the diagnosis of which is histological. They occur secondarily to activating mutations of the KIT or PDGFRA receptors in 85% of the cases, and are di-

Authors' Contributions
All the authors testified to the care of the patient and the writing of the manuscript. The authors have read and approved the final version of the manuscript.

Conflicts of Interest
Authors do not declare any conflict of interest.