Dr. Dashuang Shi
George Washington University, USA
Associate Research Professor
Email: dshi@cnmcresearch.org
Qualifications
1997 Ph.D., University of Sydney, Chemistry
1987 M.S., Xiamen University, Chemistry
1983 B.S., Xiamen University, Chemistry
Publications (selected)
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Wu, J., Shen, E., Shi, D., Sun, Z. and Cai, T. (2012) Identification of a novel Cys146X mutation of SOD1 in familial amyotrophic lateral sclerosis by whole-exome sequencing. Genetic in Medicine, 14, in press.
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Wei, J., Xue, Y., Wu, L., Ma, J., Yi, X., Zhang, J., Lu, B., Li, C., Shi, D., Shi, S., Feng, X. and Cai, T. (2012) Analysis of large phenotypic cariability of EEC and SHFM4 syndromes caused by K193E mutation of the TP63 gene. Plos One, 7:e35337.
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Shi, D., Yu, X., Zhao, G., Ho, J., Lu, S., Allewell, N.M. and Tuchman, M. (2012) Crystal structure and biochemical properties of putrescine carbamoyltransferase from Enterococcus faecalis: assembly, active site and allosteric regulation. Proteins, 80, 1436-1447.
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Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Zhao, G., Haskins, N., Allewell, N.M. and Tuchman, M. (2011) A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris. PLoS One, 6:e28825.
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Li, Y., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M. and Shi, D. (2011) The ygeW encoded protein from Escherichia coli is a knotted ancestral catabolic transcarbamylase, Proteins, 79, 2327-2334.
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Li, Y., Yu, X., Ho, J., Fushman, D., Allewell, N.M., Tuchman, M. and Shi, D. (2010) Reversible post-translational carboxylation modulates the enzymatic activity of N-acetyl-L-ornithine transcarbamylase. Biochemistry, 49, 6887-6895.
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Caldovic, L., Mew, N.A., Shi, D., Morizono, H., Yudkoff, M., Tuchman, M. (2010) N-acetylglutamate synthase: structure, function and defects. Molecular Genetics and Metabolism, 199, S13-S19.
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Ou, W., King, H., Delisle, J., Shi, D., Wilson, C.A. (2009) Phenylalanines at positions 88 and 159 of Ebolavirus envelope glycoprotein differentially impact envelope function. Virology, 396, 135-142.
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Min, Li., Jin, Z., Caldovic, L., Morizono, H., Allewell, N.M., Tuchman, M. and Shi, D. (2009) Mechanism of allosteric inhibition of N-acetyl-glutamate synthase by L-arginine. J. Biol. Chem. 284, 4873-4880.
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Shi, D., Sagar, V., Jin, Z., Yu, X., Caldovic, L., Morizono, H., Allewell, N. M. and Tuchman, M. (2008) The Crystal Structure of N-Acetyl-L-glutamate Synthase from Neisseria gonorrhoeae Provides Insights into Mechanisms of Catalysis and Regulation. J. Biol. Chem. 283, 7176-7184.
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Shi, D., Yu, X., Cabrera-Luque, J., Chen, T., Roth, L., Morizono, H., Allwell, N. M. and Tuchman, M. (2007) A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylase. Protein Sci. 16, 1689-1699.
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Qu, Q., Morizono, H., Shi, D., Tuchman, M. and Caldovic, L. (2007) A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase. BMC Biochem. 8, 4.
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Shi, D., Yu, X., Roth, L., Tuchman, M. and Allewell, N.M. (2007) Structure of A Novel N-Acetyl-L-citrulline deacetylase from Xanthomonas campestris. Biophys. Chem. 126, 86-93.
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Shi D, Caldovic L, Jin Z, Yu X, Qu Q, Roth L, Morizono H, Hathout Y, Allewell NM, Tuchman M. (2006) Expression, crystallization and preliminary crystallographic studies of a novel bifunctional N-acetylglutamate synthase/kinase from Xanthomonas campestris homologous to vertebrate N-acetylglutamate synthase. Acta Crystallograph Sect F Struct Biol Cryst Commun. 62, 1218-22.
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Shi, D., Morizono, H., Cabrera-Luque, J., Yu, X., Roth, L., Malamy, M.H., Allewell, N.M. and Tuchman, M.. (2006) Structure and mechanism of a novel N-succinyl-L-ornithine trasncarbamoylase in arginine biosynthesis of Bacteroides fragilis. J. Biol. Chem. 281, 20623-20631.
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Shi, D., Yu, X., Roth, L., Morizono, H., Tuchman, M. and Allewell, N.M, (2006) Structures of N-acetylornithine transcarbamoylase from Xanthomonas campestris complexed with substrates and substrate analogues imply mechanisms for substrate binding and catalysis. Proteins, 634, 532-542.
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Morizono, H., Cabrera-Luque, J., Shi, D., Gallegos, R., Yamaguchi, S., Yu, X., Allewell, N.M., Malamy, M.H. and Tuchman, M. (2006) Acetylornithine transcarbamylase: A novel enzyme in arginine biosynthesis. J. Bacteriol. 188, 2974-2982.
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Caldovic, L., Lopez, G.Y., Haskins, N., Panglao, M., Shi, D., Morizono, H. and Tuchman, M. (2006) Biochemical Properties of Recombinant Human and Mouse N-acetylglutamate Synthase. Mol. Genet. Metab. 87, 226-232.
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Shi, D., Yu, X., Roth, L., Morizono, H., Yetrib, H., Allewell, N.M. and Tuchman, M. (2005) Expression, purification, crystallization and preliminary X-ray crystallographic studies of a novel acetylcitrulline deacetylase from Xanthomonas campestris. Acta Crystallogr. F61, 676-679.
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Shi, D., Morizono, H., Yu, X., Roth, L., Caldovic, L., Allewell, N.M., Malamy, M.H. and Tuchman M. (2005) Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria. J. Biol. Chem. 280, 14366-14369.
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Caldovic, L, Morizono, H, Panglao, M.G., Lopez, G.Y., Shi, D., Summar, M.L. and Tuchman M. (2005) Late onset N-acetylglutamate synthase deficiency caused by hypomorphic alleles. Hum. Mutat. 25, 293-298.
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Morizono, H., Caldovic, L., Shi, D. and Tuchman, M. (2004) Mammalian N-acetylglutamate. Molecular Genetics and Metabolism. 81, S4-S11.
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Shi, D., Gallegos, R., DePonte, J., Morizono, H., Yu, X., Allewell, N.M., Malamy, M. and Tuchman, M. (2002). Crystal structure of a transcarbamylase-like protein from bacteroides fragilis at 2.0Å resolution. J. Mol. Biol. 320, 899-908.
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Caldovic, L., Morizono, H., Panglao, M.G., Gallegos, R., Yu, X., Shi, D., Allewell, N.M., Malamy, M.H. and Tuchman, M. (2002) Cloning and expression of the human N-acetylglutamate synthase gene. Biochem. Biophy. Res. Comm. 299, 581-586.
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Caldovic, L., Morizono, H., Yu, X., Thompson, M., Shi, D., Gallegos, R., Allewell, N.M., Malamy, M.H. and Tuchman, M. (2002) Identification, cloning and expression of the mouse N-acetylglutamate synthase gene. Biochem. J. 364, 825-831.
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