Biography

Dr. Dashuang Shi

George Washington University, USA

Associate Research Professor


Email: dshi@cnmcresearch.org


Qualifications

1997 Ph.D., University of Sydney, Chemistry

1987 M.S., Xiamen University, Chemistry

1983 B.S., Xiamen University, Chemistry


Publications (selected)

  1. Wu, J., Shen, E., Shi, D., Sun, Z. and Cai, T. (2012) Identification of a novel Cys146X mutation of SOD1 in familial amyotrophic lateral sclerosis by whole-exome sequencing. Genetic in Medicine, 14, in press.
  2. Wei, J., Xue, Y., Wu, L., Ma, J., Yi, X., Zhang, J., Lu, B., Li, C., Shi, D., Shi, S., Feng, X. and Cai, T. (2012) Analysis of large phenotypic cariability of EEC and SHFM4 syndromes caused by K193E mutation of the TP63 gene. Plos One, 7:e35337.
  3. Shi, D., Yu, X., Zhao, G., Ho, J., Lu, S., Allewell, N.M. and Tuchman, M. (2012) Crystal structure and biochemical properties of putrescine carbamoyltransferase from Enterococcus faecalis: assembly, active site and allosteric regulation. Proteins, 80, 1436-1447.
  4. Shi, D., Li, Y., Cabrera-Luque, J., Jin, Z., Yu, X., Zhao, G., Haskins, N., Allewell, N.M. and Tuchman, M. (2011) A Novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris. PLoS One, 6:e28825.
  5. Li, Y., Jin, Z., Yu, X., Allewell, N.M., Tuchman, M. and Shi, D. (2011) The ygeW encoded protein from Escherichia coli is a knotted ancestral catabolic transcarbamylase, Proteins, 79, 2327-2334.
  6. Li, Y., Yu, X., Ho, J., Fushman, D., Allewell, N.M., Tuchman, M. and Shi, D. (2010) Reversible post-translational carboxylation modulates the enzymatic activity of N-acetyl-L-ornithine transcarbamylase. Biochemistry, 49, 6887-6895.
  7. Caldovic, L., Mew, N.A., Shi, D., Morizono, H., Yudkoff, M., Tuchman, M. (2010) N-acetylglutamate synthase: structure, function and defects. Molecular Genetics and Metabolism, 199, S13-S19.
  8. Ou, W., King, H., Delisle, J., Shi, D., Wilson, C.A. (2009) Phenylalanines at positions 88 and 159 of Ebolavirus envelope glycoprotein differentially impact envelope function. Virology, 396, 135-142.
  9. Min, Li., Jin, Z., Caldovic, L., Morizono, H., Allewell, N.M., Tuchman, M. and Shi, D. (2009) Mechanism of allosteric inhibition of N-acetyl-glutamate synthase by L-arginine. J. Biol. Chem. 284, 4873-4880.
  10. Shi, D., Sagar, V., Jin, Z., Yu, X., Caldovic, L., Morizono, H., Allewell, N. M. and Tuchman, M. (2008) The Crystal Structure of N-Acetyl-L-glutamate Synthase from Neisseria gonorrhoeae Provides Insights into Mechanisms of Catalysis and Regulation. J. Biol. Chem. 283, 7176-7184.
  11. Shi, D., Yu, X., Cabrera-Luque, J., Chen, T., Roth, L., Morizono, H., Allwell, N. M. and Tuchman, M. (2007) A single mutation in the active site swaps the substrate specificity of N-acetyl-L-ornithine transcarbamylase and N-succinyl-L-ornithine transcarbamylase. Protein Sci. 16, 1689-1699.
  12. Qu, Q., Morizono, H., Shi, D., Tuchman, M. and Caldovic, L. (2007) A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase. BMC Biochem. 8, 4.
  13. Shi, D., Yu, X., Roth, L., Tuchman, M. and Allewell, N.M. (2007) Structure of A Novel N-Acetyl-L-citrulline deacetylase from Xanthomonas campestris. Biophys. Chem. 126, 86-93.
  14. Shi D, Caldovic L, Jin Z, Yu X, Qu Q, Roth L, Morizono H, Hathout Y, Allewell NM, Tuchman M. (2006) Expression, crystallization and preliminary crystallographic studies of a novel bifunctional N-acetylglutamate synthase/kinase from Xanthomonas campestris homologous to vertebrate N-acetylglutamate synthase. Acta Crystallograph Sect F Struct Biol Cryst Commun. 62, 1218-22.
  15. Shi, D., Morizono, H., Cabrera-Luque, J., Yu, X., Roth, L., Malamy, M.H., Allewell, N.M. and Tuchman, M.. (2006) Structure and mechanism of a novel N-succinyl-L-ornithine trasncarbamoylase in arginine biosynthesis of Bacteroides fragilis. J. Biol. Chem. 281, 20623-20631.
  16. Shi, D., Yu, X., Roth, L., Morizono, H., Tuchman, M. and Allewell, N.M, (2006) Structures of N-acetylornithine transcarbamoylase from Xanthomonas campestris complexed with substrates and substrate analogues imply mechanisms for substrate binding and catalysis. Proteins, 634, 532-542.
  17. Morizono, H., Cabrera-Luque, J., Shi, D., Gallegos, R., Yamaguchi, S., Yu, X., Allewell, N.M., Malamy, M.H. and Tuchman, M. (2006) Acetylornithine transcarbamylase: A novel enzyme in arginine biosynthesis. J. Bacteriol. 188, 2974-2982.
  18. Caldovic, L., Lopez, G.Y., Haskins, N., Panglao, M., Shi, D., Morizono, H. and Tuchman, M. (2006) Biochemical Properties of Recombinant Human and Mouse N-acetylglutamate Synthase. Mol. Genet. Metab. 87, 226-232.
  19. Shi, D., Yu, X., Roth, L., Morizono, H., Yetrib, H., Allewell, N.M. and Tuchman, M. (2005) Expression, purification, crystallization and preliminary X-ray crystallographic studies of a novel acetylcitrulline deacetylase from Xanthomonas campestris. Acta Crystallogr. F61, 676-679.
  20. Shi, D., Morizono, H., Yu, X., Roth, L., Caldovic, L., Allewell, N.M., Malamy, M.H. and Tuchman M. (2005) Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria. J. Biol. Chem. 280, 14366-14369.
  21. Caldovic, L, Morizono, H, Panglao, M.G., Lopez, G.Y., Shi, D., Summar, M.L. and Tuchman M. (2005) Late onset N-acetylglutamate synthase deficiency caused by hypomorphic alleles. Hum. Mutat. 25, 293-298.
  22. Morizono, H., Caldovic, L., Shi, D. and Tuchman, M. (2004) Mammalian N-acetylglutamate. Molecular Genetics and Metabolism. 81, S4-S11.
  23. Shi, D., Gallegos, R., DePonte, J., Morizono, H., Yu, X., Allewell, N.M., Malamy, M. and Tuchman, M. (2002). Crystal structure of a transcarbamylase-like protein from bacteroides fragilis at 2.0Å resolution. J. Mol. Biol. 320, 899-908.
  24. Caldovic, L., Morizono, H., Panglao, M.G., Gallegos, R., Yu, X., Shi, D., Allewell, N.M., Malamy, M.H. and Tuchman, M. (2002) Cloning and expression of the human N-acetylglutamate synthase gene. Biochem. Biophy. Res. Comm. 299, 581-586.
  25. Caldovic, L., Morizono, H., Yu, X., Thompson, M., Shi, D., Gallegos, R., Allewell, N.M., Malamy, M.H. and Tuchman, M. (2002) Identification, cloning and expression of the mouse N-acetylglutamate synthase gene. Biochem. J. 364, 825-831.



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