Prof. Roberto
Fernández-Lafuente
ICP, Consejo Superior de Investigaciones
Científicas, Spain
Email: rfl@icp.csic.es
Qualifications
PhD in Sciences by UAM
Degree in Biology by UCM
Publications (Selected)
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Poppe, J.K., Matte, C.R., Do Carmo Ruaro
Peralba, M., Fernandez-Lafuente, R., Rodrigues, R.C., Ayub, M.A.Z., Optimization
of ethyl ester production from olive and palm oils using mixtures of
immobilized lipases, (2015) Applied Catalysis A: General, 490, pp. 50-56. Cited
1 time.
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Rueda, N., Dos Santos, J.C.S., Torres, R.,
Ortiz, C., Barbosa, O., Fernandez-Lafuente, R., Improved performance of lipases
immobilized on heterofunctional octyl-glyoxyl agarose beads, (2015) RSC
Advances, 5 (15), pp. 11212-11222. Cited 2 times.
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Barbosa, O., Ortiz, C., Berenguer-Murcia, Á.,
Torres, R., Rodrigues, R.C., Fernandez-Lafuente, R.Strategies for the one-step
immobilization-purification of enzymes as industrial biocatalysts,(2015)
Biotechnology Advances, 33 (5), pp. 435-456.
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Dos Santos, J.C.S., Rueda, N., Barbosa, O.,
Fernández-Sánchez, J.F., Medina-Castillo, A.L., Ramón-Márquez, T.,
Arias-Martos, M.C., Millán-Linares, M.C., Pedroche, J., Yust, M.D.M.,
Gonçalves, L.R.B., Fernandez-Lafuente, R., Characterization of supports
activated with divinyl sulfone as a tool to immobilize and stabilize enzymes
via multipoint covalent attachment. Application to chymotrypsin,(2015) RSC
Advances, 5 (27), pp. 20639-20649. Cited 3 times.
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Paludo, N., Alves, J.S., Altmann, C., Ayub,
M.A.Z., Fernandez-Lafuente, R., Rodrigues, R.C., The combined use of ultrasound
and molecular sieves improves the synthesis of ethyl butyrate catalyzed by
immobilized Thermomyces lanuginosus lipase, (2015) Ultrasonics Sonochemistry,
22, pp. 89-94. Cited 2 times.
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Dos Santos, J.C.S., Rueda, N., Sanchez, A.,
Villalonga, R., Gonçalves, L.R.B., Fernandez-Lafuente, R., Versatility of
divinylsulfone supports permits the tuning of CALB properties during its
immobilization, (2015) RSC Advances, 5 (45), pp. 35801-35810. Cited 1 time.
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Alves, J.S., Vieira, N.S., Cunha, A.S.,
Silva, A.M., Záchia Ayub, M.A., Fernandez-Lafuente, R., Rodrigues, R.C., Combi-lipase
for heterogeneous substrates: A new approach for hydrolysis of soybean oil
using mixtures of biocatalysts, (2014) RSC Advances, 4 (14), pp. 6863-6868.
Cited 4 times.
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Barbosa, O., Ortiz, C., Berenguer-Murcia, A.,
Torres, R., Rodrigues, R.C., Fernandez-Lafuente, R., Glutaraldehyde in
bio-catalysts design: A useful crosslinker and a versatile tool in enzyme
immobilization, (2014) RSC Advances, 4 (4), pp. 1583-1600. Cited 41 times.
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Dos Santos, J.C.S., Garcia-Galan, C.,
Rodrigues, R.C., De Sant'Ana, H.B., Gonçalves, L.R.B., Fernandez-Lafuente, R., Stabilizing
hyperactivated lecitase structures through physical treatment with ionic
polymers, (2014) Process Biochemistry, 49 (9), pp. 1511-1515. Cited 4 times.
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Barbosa, O., Torres, R., Ortiz, C.,
Berenguer-Murcia, A., Rodrigues, R.C., Fernandez-Lafuente, R., Heterofunctional
supports in enzyme immobilization: From traditional immobilization protocols to
opportunities in tuning enzyme properties, (2013) Biomacromolecules, 14 (8), pp.
2433-2462. Cited 57 times.
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Rodrigues, R.C., Ortiz, C., Berenguer-Murcia,
A., Torres, R., Fernández-Lafuente, R., Modifying enzyme activity and selectivity by
immobilization, (2013) Chemical Society Reviews, 42 (15), pp. 6290-6307. Cited
209 times.
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Barbosa, O., Torres, R., Ortiz, C.,
Fernandez-Lafuente, R., Versatility of glutaraldehyde to immobilize lipases:
Effect of the immobilization protocol on the properties of lipase B from
Candida Antarctica, (2012) Process Biochemistry, 47 (8), pp. 1220-1227. Cited
36 times.
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Garcia-Galan, C., Berenguer-Murcia, A.,
Fernandez-Lafuente, R., Rodrigues, R.C., Potential of different enzyme
immobilization strategies to improve enzyme performance, (2011) Advanced
Synthesis and Catalysis, 353 (16), pp. 2885-2904. Cited 264 times.
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Rodrigues, R.C., Berenguer-Murcia, Á.,
Fernandez-Lafuente, R.,Coupling chemical modification and immobilization to
improve the catalytic performance of enzymes, (2011) Advanced Synthesis and
Catalysis, 353 (13), pp. 2216-2238. Cited 89 times.
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Hernandez, K., Fernandez-Lafuente, R., Control
of protein immobilization: Coupling immobilization and site-directed
mutagenesis to improve biocatalyst or biosensor performance, (2011) Enzyme and
Microbial Technology, 48 (2), pp. 107-122. Cited 175 times.