By using psychological and physiological indicators, the effects on the body of three-dimensional (3D) movies containing improper settings were evaluated with 139 university students. The experiment consisted of two sessions: 1) a 3D movie containing improper 3D settings was compared with a 2D movie containing only the rightside images presented to both eyes, and 2) the original 3D movie was compared with the same 3D movie altered to contain improper 3D settings. The results of this experiment demonstrated clear deterioration of the subjective psychological indicators (degree of motion sickness after watching the movies and comfort level at 1-min intervals during the movie) with respect to the 3D movie containing improper settings. On the other hand, the physiological indicators (LF/HF ratio indicating the status of the autonomic nervous system) changed as a result of watching a 3D movie, but were unaffected by the presence or absence of improper 3D settings.