Advances in Bioscience and Biotechnology
Vol.4 No.5(2013), Paper ID 32262, 7
pages
DOI:10.4236/abb.2013.45084
High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast Pichia pastoris
Tatsuro Shibui, Keisuke Bando, Satoru Misawa
Food Biotechnology Laboratory, School of Food Sciences, Nippon Veterinary and Life Science University, 2-17-5 Sakai, Musashi- no-Shi, Tokyo, Japan
API Corporation, Bio and Chemical Process Discovery Laboratory, 1000 Kamoshida-Cho, Aoba-Ku, Yokohama, Japan
Mitsubishi Chemical Group Science and Technology Research Center, 1000 Kamoshida-Cho, Aoba-Ku, Yokohama, Japan
Copyright © 2013 Tatsuro Shibui, Keisuke Bando, Satoru Misawa et al. This is
an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any
medium, provided the original work is properly cited.
How to Cite this Article
Shibui, T. , Bando, K. and Misawa, S. (2013) High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast
Pichia pastoris.
Advances in Bioscience and Biotechnology,
4, 640-646. doi:
10.4236/abb.2013.45084.