Trehalose and Sucrose Osmolytes Accumulated by Algae as Potential Raw Material for Bioethanol
178
with increasing NaCl concentration, and was inhibited at
concentrations higher than 0.4 M for all strains.
After the osmotic stress of Chlorella culture, sugar
production increased 14-fold in 0.4 M NaCl after 24 h.
The main osmolyte identified was sucrose; however, tre-
halose was also accumulated (1.93 and 0.019 mg/ bioma-
sse dw, respectively). These results show the potential use
of algae as sugar resource of fermetable sugars and the
use of osmotic stress to increase the total sugar produc-
tion. This work shows also trehalose accumulation as
osmolyte by Chlorella.
5. Acknowledgements
Funding by Instituto Politecnico Nacional PIFI-20100242.
The microscopy studies and strain identificactions
were led by Dr. Ma. Esther Meave from Univesidad Au-
tónoma Metropolitana. Iztapalapa, Mexico.
HPLC analyses were made at the Central Analítica,
Depto. de Biotecnología. CINVESTAV-IPN and directed
by Elvira Rios, Cirino Rojas, and Gustavo Medina.
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