Y.-C. LIN ET AL.
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Table 1. Surface modification and protein binding effect.
chip # 1 # 2 # 3 # 4
Surface modification time (minute) 15 30 45 60
BSA binding time ( minute) 30 30 30 30
BSA frequency shift (Hz) 117.3 136.8 137.8138.0
Efficiency (%) 3.75 4.37 4.354.65
Adsorption mass (ng) 314 366 368369
4. Results and Discussion
The frequency difference after BSA injection for 4 QCM
chips with modification time of 15, 30, 45 and 60 min-
utes, respectively, is list in Table 1. Since sulfide could
bind BSA and adsorb the mass on the surface of the chip,
the resonant frequency would degrade. More BSA be
binded, larger frequency difference will be. The fre-
quency difference were measured as 117.3 Hz, 136.8 Hz,
137.8 Hz and 138.0 Hz for the 15, 30, 45 and 60 minutes,
respectively. The efficiency is defined as the frequency
difference divided with the initial deionized water reso-
nant frequency. The results are 3.75%, 4.37%, 4.35% and
4.65%, respectively. So, the optimum sulfide modifica-
tion time for BSA protein QCM biosensor is about 30
minutes.
5. Conclusions
In this study, we present different surface modification
processing time to allow sulfide molecules to be immobi-
lized to golden electrode for QCM protein sensor. The
surface modification was also tested by BSA binding
measurement. The optimum surface modification proc-
essing time is detected as 30 minutes.
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