J. Biomedical Science and Engineering, 2010, 3, 625-632
doi:10.4236/jbise.2010.36085 Published Online June 2010 (http://www.SciRP.org/journal/jbise/
JBiSE
).
Published Online June 2010 in SciRes. http://www.scirp.org/journal/jbise
Mesoscopic relaxation time of dynamic image correlation
spectroscopy
Kyongok Kang
Forschungszentrum Juelich, Weiche Materie (Soft Matter), Juelich, Germany.
Email: k.kang@fz-juelich.de
Received 16 February 2010; revised 20 March 2010; accepted 28 March 2010.
ABSTRACT
Dynamical images contain useful information of how
the objects behave in time and space. When the sys-
tem is in biological fluids, the motion of the object is
much over-damped; the relaxation time is the char-
acteristics in a diffusive time scale. We have found
dynamical states of melting and forming of small
nematic domains (1030 μm) that are exhibited in
the suspensions of fd-viruses under applied AC elec-
tric field amplitude at low frequency. Dynamic image
correlation function is used for extracting the mes-
oscopic relaxation times of the dynamical states, which
can be employed as an application to other dynamic
imaging process of biologically relevant soft con-
densed matter and biomedical systems.
Keywords: Relaxation Time; Image Correlation
Spectroscopy; Dynamical states
1. INTRODUCTION
Dynamic contrast-enhanced MRI (magnetic resonance
imaging) is widely used for early detection of breast
tumors, where the kinetics of signal variation are moni-
tored after an injection of a paramagnetic contrast me-
dium to distinguish between benign and malignant breast
lesions [1]. The studies of microvasculature and micro-
circulation are useful in the sense that the solid tumor
objects can disturb the regional blood flow, and influ-
ences the lack of linkage in a basement membrane. It is
still a challenge to generalize the estimation of a regional
blood flow and capillary permeability as volume fraction
of the intravascular flow in breast tumors [1]. According
to the authors in [1], the important criterions of reliable
signal contrast (or relaxation times) are dependent on
two assumptions; one is a negligible relaxavity of pro-
tons in different tissue compartment, and the other is
much faster diffusion of water within a tissue and the
exchange rate between them. The relaxation of bulk
magnetization can be found and described by a single
relaxation time [2]. These assumptions are feasible for a
typical diffusion coefficient for water in soft tissues, and
its relevant mean-squared displacement is reported as
typical value of 30-60 μm size within about 125 ms re-
covery time [2]. Depending on the biomedical system,
for instance, gray and white matter in the brain, heart, and
kidney are in a relatively slow relaxation process (relaxa-
tion time as about 1/s) within tissue and blood stream, as
compared to other systems as liver and spleen [2]. Thus
as far as the contrast agent is efficiently fast enough, then
the slow process of water permeability can be negligible
in terms of the exchange rates between different com-
partments for early detection of malignant tumor cells.
Thus for an intermediate and slow time process, the
single exponent time constant is convenient to character-
ize most biological relevant experimental parameters.
When the time-evolutionary information is engaged
with a local space, it is somewhat complicated to distin-
guish between the time, and space-variable. For the sys-
tem that contains self-mimicking sizes or shapes of ob-
jects (or images), but with different temporal events, it is
extremely useful to predict what will happen after a cer-
tain time steps of events. Relatively large-time scale,
such as astronomical or geographical-time, and macro-
scopic length are less relevant to predict “probable” events
in mesoscopic time scale. Most physiochemical envi-
ronments, i.e., medical, biochemical and biophysical
issues are more engaged with a shorter-time interval. In
the latter case, the system of interested objects is gov-
erned by thermodynamics and hydrodynamics, commonly
in between the microscopic and macroscopic approach.
As far as in time-correlation aspect, this mesos-copic time
scale can be influenced by globally metastable but par-
tially (or locally) in non-equilibrium states. Thus time-
correlation is still a challenge in mesoscopic length and
time scale and in the speculation, nevertheless which is
ambient in most soft matters. The demanding task of
predicting “forward” temporal events relies on the com-
plexity of dynamical entities (or inhomogenieties) them-
selves that evolve in the physiological situations (or en-
626 K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632
Copyright © 2010 SciRes. JBiSE
vironments). Based on an appropriate statistical sam-
pling, one can identify the quantitatively possible “ob-
servable” physiological objects at a time. Further esti-
mation of the next-time expected value can be governed
by the relaxation rate of the dynamical variables. Re-
cently, a luminescent conjugate polyelectrolyte (LCP) is
used for monitoring the amyloid plaques that can de-
velop at various stages in human brain as known for
Alzheimer’s disease [3]. To diagnose Alzheimer’s disease
in the earlier stage, image color maps can be monitored
as a non-invasive method. Image correlation spectros-
copy is also used for protein dynamics on the cell sur-
face and live-cell imaging to extract the information of
aggregation and number density [4]. The desirable fea-
ture of the imaging correlation spectroscopy is suggested
to achieve the diffusion coefficients and velocity for pro-
bing proteins in living cells [5]. Employing the medical
imaging for the kinetic pathways of decaying or growing
of bad cellular propagation is remained as a highly valu-
able issue. Much efficient and conclusive biomedical per-
formance is needed to characterize the “urgency” in a
simple and an “integrated” manner in time-resolving
frames. Mesoscopic time and length scale imaging cor-
relation spectroscopy could have its applications within
biomedical technology of soft-tissues. The main difficul-
ties to overcome will probably be related to small optical
density differences. In case of soft-organs or tissues that
consist of fibrous structures in the presence of multiva-
lent ionic liquids, structures can be imaged through the
birefringent nature of the fibrous structures. Then the ori-
entation contrast can be conveniently visualized between
crossed polarizers, which can be combined with time-
lapsed imaging processing.
We employ charged fibrous viruses (fd) suspensions
to explore low frequency electric field-induced phase tran-
sitions due to their anisotropic conformations (or configu-
rations) under optical axis. Especially, the partial ordering
is found as increased with increasing field amplitude
[6,7]. This kind of fibrous structure can be also found in
other biological materials, such as in optical nerve cells,
living polymers of F-actin and myosin fibers. Particu-
larly optical nerve cells are directly engaged with our
human brain “cognitive” central nervous system. Inter-
esting, it is already known fact that there are object rec-
ognition activities with low frequency oscillations
(called Gamma band, ~ 40 Hz) in human brain system
[8,9]. It is the purpose of this paper that we introduce our
working system of dynamic image correlation spectros-
copy to predict the mesoscopic relaxation time of dy-
namical states of small nematic domains (~ 10-30 μm
size) that consist of charged fd-viruses particles.
2. METHOD AND DATA ANALYSIS
2.1. Sample Preparation
Sample preparation is done by following the standard
biological protocols [10] to prepare the fd-virus suspen-
sions, from the XL1blue strain of E. coli as the host bac-
teria. The virus particles are purified by repeated cen-
trifugation 105 g for 5 hours and re-dispersed in Tris/
HCl buffers with varying concentration, depending on
the final ionic strength. Bacteriophage fd is a rod-like
macromolecule with a length of 880 nm, a bare diameter
of D = 6.6 nm, a persistence length at high salt concen-
tration of P = 2200 nm, and a molecular weight of M.W.
~ 1.64 × g/mol. Much detailed information of mo-
lecular structures and dynamics of fd coat proteins are
discussed in earlier times by means of scanning trans-
mission electron microscope and solid-state NMR method
[11-14]. Hydrodynamic properties and structure of fd-
viruses are measured by transient electric birefringence,
and low-angle intensity fluctuations spectroscopy [15].
7
10
We prepare fd-virus suspensions at a given low ionic
strength by osmotic equilibration (or dialysis) for 2 days
with an analytical concentration of 1.6 mM ionic stren-
gth of Tris/HCl buffer. The same buffer is used to dilute
the suspensions (by typically 20 %) to the desired fd
concentration. The ionic strength of the osmotic reser-
voirs is the ionic strength from which the electrostatic
Debye-Huekel screening length is calculated. Further-
more, osmotic equilibration assures that the pH of the
suspensions is equal to those of the osmotic reservoir.
The Tris/HCl buffers are prepared by adding a small
volume of HCl with a concentration of 1 M to a 20 mM
TRIS solution till a pH = 8.2 is attained. This buffer is
then diluted with deionized water for the preparation of
the lower Tris/HCl-buffer concentrations. For buffer
concentrations less than about 10 mM, both the ionic
strength and the pH are affected by carbon dioxide that
dissolves from the air, which in turn affects the surface
charges of the fd-virus particles. A detailed account of
the effect of dissolved carbon dioxide on the ionic
strength and pH as a function of the buffer concentration
can be found in [16]. Then the fd-concentration is meas-
ured by UV spectrometer to read optical density, or the
extinction coefficient at approximately 269 nm wave-
length.
The charge distribution on fd-virus particles has been
interpreted on the basis of different models for an fd-
virus particle from titration curves. The best fit to titra-
tion curves is found when assuming that the charges are
located only on the hydrophilic outer region of adsorbed
coat proteins, and not on the DNA strand of fd itself [17].
The iso-electric point of native fd is equal to pH ~ 4.2.
The surface charge is measured approximately nega-
tively charged as 9000e at pH = 6.9. The buffer capac-
Part of this research was supported by the EU-FP7 Network “NanoDi-
rect” (contract number CP-FP-213948-2).
K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632 627
Copyright © 2010 SciRes. JBiSE
ity of the low-ionic strength Tris/HCl buffer that we used
(for 0.16 mM salt concentration) is still sufficient to
keep the pH > 6.5 [16], despite dissolving carbon diox-
ide from the air.
2.2. Collection of Raw Image Intensity at Finite
Time Steps
The realization of morphological changes in local events
at a space is much sensitive when the system is governed
by dynamically variable quantities (or objects). As far as
the system maintains a uniquely defined phase or state
for much long-time as compared to the resolution of
appropriate time binning, a decent characteristic time
can be extracted for their relaxation behaviors. Also the
optimization of AOI (area of interests) averaging is rele-
vant to obtain “ensemble” average correlations, which
influences the good average of background in statistics
at far later time after the event occurs. Images are col-
lected with an appropriate finite time step to develop the
evolution of spatio-temporal morphological changes. De-
pending on the nature of dynamical states in the physical
system, we have found that the resolution of time binning
in sampling has to be optimized such that at least 3-10
times smaller than the actual time of dynamical events.
For example, the dynamical state itself evolves in few
tens of second, then appropriate time binning should be
treated within a second as a time step.
Intensity-time traces for dynamical images are then
constructed from the intensity map in 2-dimensional area,
which normally depends on the optimization between
the quality of the CCD camera and area of interest (AOI)
in system. Figure 1 shows the example of collections of
dynamical images at few seconds of time steps. Here the
sampling time is every 1 sec, and total duration time is
1800 sec. The dynamical morphologies are driven by
external AC electric field at a low frequency (of 10 Hz)
and amplitude (of 3.2 V/mm). This dynamical state is
still slowly changing in time and space, so-called slow
dynamical state as DS state. The change of visual obser-
vation of these slow dynamical states is typically oc-
curred within 30 sec. By applying higher field amplitude,
the slow dynamical state, DS transits to the fast dynami-
cal state, i.e., Df, where we have chosen multi-runs short
time collections, as time binning as 300 ms and duration
time for 60 s and 5 runs averaging. Due to the speed
limitation of our camera, normally the camera setting
ought to be optimized between fast speed and high reso
lution option. We have optimized the CCD camera set as
fast camera exposure time since the dynamical state do-
mains are quite visible in CCD pixel by pixel.
We are then able to capture the dynamical events at
least 3 or 4 different time steps within a sec temporal
event in the fast dynamical, Df -state.
t = 0t = 3 s
t = 5 st = 7 s
t = 9 st = 11 s
t = 13 st = 15 s
t = 0t = 3 s
t = 5 st = 7 s
t = 9 st = 11 s
t = 13 st = 15 s
Figure 1. Temporal images of dynamical states that are in-
duced by external electric AC field amplitude (of 3.2 V/mm) at
a low frequency (of 10Hz). Field of view is of 400 μm × 300
μm, and the dotted lines are guiding eyes for detecting small
neamtic domains of fd-virus particles. White circle is presented
for tracing particular region of spatiotemporal images for dy-
namical states in each frame. Dynamical image correlation
function is calculated as a resolution of single pixel averaging
in 2-d over 1800 s duration time, where the flexible time reso-
lution can be adapted as 0.3 s, 1 s, for fats and slow dynamical
states, respectively.
It turned out that this time binning may not be fast
enough to characterize “completely” in the fast dynami-
cal states, where the relaxation behaviors could be real-
ized at much microscopic time scale. As a consequence,
the background value turned out to be occasionally be-
low zero, indicating that the time step is too large to
carry on the local events correlations. It would be desir-
able when the time binning of the sampling time has to
be tested with the background value to present as the
dynamical state. Also it has to be chosen as much faster
exposure CCD camera to ensure the exposure time of
camera setting is much faster than the actual duration
time for measurement. Thus by allowing the time step is
much smaller than the change of visual morphological
changes in dynamical states, time-lapsed images are re-
corded within the chosen frame setting, and saved (or
exported) as time-sequential bmp files with 8 bit gray
data that is mostly common format in handling image
objects.
628 K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632
Copyright © 2010 SciRes. JBiSE
2.3. Construction of Modified Intensity Matrix
To construct the intensity-time traces of images, the in-
tensity matrix values of every pixel AOI (1 pixel × 1
pixel) are reconstructed for dynamic image correlation
function, as a “readable” Ascii format to correlate with
different time-frames of AOI. The modified intensity ma-
trix is defined as the subtracted intensity average value of
AOI pixel for every time frame from the raw intensity
data. Figure 2(b) shows the simple sketch of the en-
hanced contrast between “bright” and “dark” intensity
AOI pixels.
The modified intensity matrix is then reconstructed as
a building block of individual time frame, such that the
each time frame image is subtracted with the overall
average intensity value of the initial time frame, as can
be seen in Figure 2(b). Then enhanced contrast is sketched
as a bright (white) and dark (or black) unit pixel area,
against the mean average of a gray color code. In princi-
ple, for a long time of duration, the change of the dy-
namical events can be completely “independent”, so that
at far later time after the event occurs, the time-correla-
tion of dynamical states is expected to be ” non-corre-
lated” at the end of time event. This is briefly depicted in
the long time later after an event occurs in Figure 2(b),
which has now shown flipped from “white” to “black”
and “black” to “white” code.
I(t = 0)
I
t
I
t
+
-
0
(1)nt
t
2t
(2)nt
(a)
(b)
I(t = 0)
I
t
I
t
+
-
0
(1)nt
t
2t
(2)nt
I(t = 0)
I
t
I
t
I
t
I
t
+
-
0
+
-
0
(1)nt
t
2t
(2)nt
(a)
(b)
Figure 2. Simple sketches of the procedure of data analysis: (a)
A time-lapsed sequence of raw images with a “readable” inten-
sity matrix as 8 bit gray bitmap images. (b) A time-lapsed se-
quence of modified intensity matrix as a subtracted average
intensity value at each time frame. Bright and dark intensity is
contrasted in this modified intensity matrix. The resultant in-
tensity traces over time is presented as typical slow and fast
oscillations, and white, gray, and black unit cell represents the
sign of the intensity values, notified in the modified intensity
map in (b).
The right panels of Figure 2(b) are shown for the in-
tensitytime traces for both slow and fast dynamical
state, up and down figures, respectively. The apparent
intensity oscillations are due to the spatio-temporal mo-
tion of dynamical states of the small nematic domains
(of fd-virus particles).
2.4. Calculation of the Image Correlation
Function
The correlation function is defined as

2
() ()(0) (0)
()
(0) (0)
It ItII
Ct
II
 
 
(1)
where I is the transmitted intensity at a given pixel and
the brackets <…> denote averaging over all pixels. We
made a program to calculate the dynamical image corre-
lation function. We have found the best performance of
the intensity averaging is 1 pixel by 1 pixel, as compared
to 5 pixels by 5 pixels (or 10 pixels by 10 pixels) aver-
aging. This may be related to a low magnification objec-
tive lens (10X objective) that is chosen for larger sam-
pling of AOI in our system. Figure 3(a) shows the typi-
cal correlations functions of our dynamic imaging corre-
lations as a function of increasing field amplitude (as
indicated the arrow direction). Here, the relaxation time
of dynamical states of melting and forming of small
nematic-domains of fd-virus particles is presented as a
function of applied field amplitude at a low frequency
(of 10 Hz).
3. RESULTS AND DISCUSSION
To briefly summarize the field-induced phase/state tran-
sitions of charged fibrous viruses (fd) at a low ionic
strength:
At low driving frequency and a low field amplitude,
the chirality is induced, which is seen as a “stripe” tex-
ture (as a chiral nematic, N*-phase) in depolarized light.
At higher field amplitude, this field-induced chiral-
ity is slowly disconnected and developed as smaller do-
mains that contain chiral nematics, so called as *
D
N-
phase.
Further increasing of the field amplitude, there is a
discontinuous transition between melting away of chiral-
ity and appearing of small nematic domains that are ki-
netically responding in space and time. Thus this leads to
the dynamical states as slow and fast in an increase of
the amplitude, as DS and Df -state, respectively.
Detailed electric phase/state diagrams are presented
and characterized in [6].
As one can see in Figure 3, the relaxation time is
decreased as increasing field amplitude; typical dyna-
mical image correlation functions of a low frequency
(10 Hz) are shown for varying the applied field amplitude
K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632 629
Copyright © 2010 SciRes. JBiSE
Figure 3. Typical dynamical image correlation functions of a
low frequency (10 Hz) for a fd-concentration (of 2.8 mg/ml):
Relaxation time of dynamical states is decreased as an increase
of the applied AC field amplitude as (a) 3.1 V/mm, (b) 3.5
V/mm, and (c) 3.9 V/mm. Note that there are relatively main-
taining noise distribution in the background at longer time tails
due to the disturbance of the external field.
from 3.1 V/mm, 3.5 V/mm, and 3.9 V/mm. Also the mi-
croscopic discontinuity is observed in an increase of
field amplitude at low frequency, at the transition of
chiral-nematic phase to dynamical states (around 3.3
V/mm), by means of dynamic lights scattering [6,7]. The
discontinuity may be due to the fact of “intrinsic” dif-
ferences on the microscopic dynamics between chiral-
nemtic phase and non-chiral nematic domains that are
present between isotropic and nematic coexistent phase.
At this transition, the chirality slowly disappears and at
the same time, non-chiral small nematic domains are
becoming dynamic, in terms of their melting and forma-
tion of the small non-chiral nematic domains (~ 20 μm).
Further increasing field amplitude at a low frequency,
the dynamical states of melting and forming of small
nematic domains are becoming faster. Thus kinetics of
dynamical states is enhanced by an increase of field am-
plitude, as it can be seen in Figure 4(a). The mesoscopic
relaxation times of these dynamical image correlation
functions are indeed shown as “faster” as an increase of
the field amplitude in Figure 4(b). These dynamic image
correlation functions of applied field amplitude at a low
frequency can be fitted with an exponential decay with a
stretching exponent. The solid lines are fitted with a fit-
ting function of
()exp( /)CtB At
  (2)
where 0,~ 0.50.6,0.51BA

, and τ is the meas-
ure for a characteristic time for melting and appearing
small nematic domains of fd-virus particles.
This characteristic time diverges on approach of the
chiral-phase transition on lowering the field amplitude,
in a mesoscopioc correlation time scale. Hoswever, the
transition from the DS- to- D
f -state is defined as the
point where the sharp decrease of τ with increasing field
amplitude ceases to occur. The mesoscopic relaxation
time has a tendency of saturation that the dynamics be
Figure 4. (a) Dynamical image correlation func-
tions for various AC field amplitudes at low fre-
quency (of 10 Hz) of the fd-concentration (2.8
mg/ml at 0.16 mM salt). Arrow indicates the di-
rection of an increase of field amplitude (from
3.26, 3.78, 4.09, to 5.77 V/mm). Note that there is
a very small modulation at longer time window; (b)
Results of the mesoscopic relaxation time for dy-
namical image correlation functions for a low fre-
quency (of 10 Hz).
630 K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632
Copyright © 2010 SciRes. JBiSE
comes essentially independent of the field amplitude.
Whether this is due to the lack of our limited choice of
time binning or the “intrinsic” feature of our dynamical
system is not yet clear. We also have performed dynamic
image correlation spectroscopy on approaching of the Df
-to-DS -state transition, by increasing frequency at a high
field amplitude, where “critical” slowing down behav-
iors of small nematic domains are found, where the
critical exponents can be obtained [18].
Figure 4(a) has shown the results of dynamical cor-
relation functions for various field amplitudes, where the
arrow indicates the increase of the field amplitude. Dis-
regard of the background oscillations, the relaxation time
decreases with an increase of the field amplitude. This
means that the fast dynamical states are relaxing much
faster than the slow dynamical states, as it is expected in
the time correlation method. It also maintains as a “re-
versible”, which is a good candidate for “tuning” the
dynamical states with external driving frequency. Figure
4(b) is the resultant relaxation time as a function of ap-
plied field amplitude, where the field-induced *
D
N-phase,
slow, DS, and fast dynamical Df states are shown. Note
that there are relatively “noisy” oscillations can be ob-
tained in the long time tails (see Figure 4(a)), which
may be due to the constant disturbance (or “feedback”)
of the external field. If the system is purely diffusive,
then this correlation function has zero background.
However, since the system is constantly feed backed by
external alternating current amplitude, the intensity-time
correlation function background does not go “directly”
to absolute zero, but slightly oscillates around zero, al-
though mathematically in principle should be “zero”.
Thus we speculate these small contributions of white
noise in terms of our low driving frequency as well. This
can be useful for many biological imaging processing or
biomedical electronics researches, since the signals are
too much “weak” and “dark” to detect and to carry on in
biological fluids and compartments (soft tissues).
Figure 5 indicates the zooming view of the back-
ground noise oscillations at larger time window for both
slow (in (a)), and faster (in (b)) dynamical state. As one
can see, the background oscillations are “modulated” in
a complex manner, and the quality of the frequency is
much “noisy” than the driving (or input) frequency.
In order to look at these possible oscillations in more
detail, we propose a data analysis as follows. Prepare
two independent backgrounds. One is from the measured
back ground oscillation at larger times, as shown in
Figure 6(a). The other is a “virtual” background oscilla-
tion (the sinusoidal waveform that is chosen as compa-
rable amplitude in Figure 6(a) for a frequency that is ~
1% of driving frequency.
Typically ~ 10% signal-to-noise ratio is feed into in
Figure 5. Zooming view of the background noise oscillation
in the image correlation functions at longer time- window: (a)
for slow dynamical state (3.24 V/mm), and (b) for faster dy-
namical state (3.86 V/mm).
the real correlation background noise. Then subtracted
signals from the “virtual” oscillations are shown in Fig-
ure 6(b), as given same time window, the resultant of
the background signal is shown. The Fourier transform
of the resulting signal gives the power spectrum given in
Figure 6(c). Dashed line is the estimation of the power
spectrum by using a frequency (10 Hz) pass filter. Inter-
estingly, there is a sharp major peak value found as 1%
of input driving frequency, which is good agreement of
cut-off envelop signal. Whether this analysis is sufficient
to contribute to the interpretation of the dynamics of the
melting and forming of nematic domains needs further
investigation. However, the weak background oscilla-
tions are not influenced by the choice of the temporal-
sensitivity, and indicate the intrinsic feature of field-
induced dynamical states. It would be quite intriguing to
know whether the smaller peaks in the power spectrum
(between the major peaks) in Figure 6(c) can be amplified
or modulated to tune the low frequency spectrum in the
slowly varying dynamical systems. However this is be-
yond the scope of this paper.
4. CONCLUSIONS
For a dynamical image processing, we have used an ex-
perimentally working system for the time-lapsed dy-
namic image correlation spectroscopy. Series of collected
time stacks of region of interests are chosen such that they
maintain enough collective motion of single particles.
In our experiment, typical average size of small nematic
K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632 631
Copyright © 2010 SciRes. JBiSE
100 200 300 400
-0.10
-0.05
0.00
0.05
0.10
Time [s]
C (t)
(a)
100 200 300 400
-0.10
-0.05
0.00
0.05
0.10
(b)
C_R (t)
Time [s]
-0.10 -0.050.000.050.10
0.0
1.0x10-7
2.0x10-7
3.0x10-7
(c)
Power
10 Hz Low Pass Filter on FFT2_Power
Power [a.u.]
Frequency [Hz]
0
100 200 300 400
-0.10
-0.05
0.00
0.05
0.10
Time [s]
C (t)
(a)
100 200 300 400
-0.10
-0.05
0.00
0.05
0.10
Time [s]
C (t)
(a)
100 200 300 400
-0.10
-0.05
0.00
0.05
0.10
(b)
C_R (t)
Time [s]
-0.10 -0.050.000.050.10
0.0
1.0x10-7
2.0x10-7
3.0x10-7
(c)
Power
10 Hz Low Pass Filter on FFT2_Power
Power [a.u.]
Frequency [Hz]
0
(a)
(b)
(c)
Figure 6. Further analysis of the background noise in the dy-
namical image correlation function: (a) Circle data points pre-
sent a raw background signal, which is less than 5 % of total
S/N ratio, and the thin solid line data is for an input signal with
an optimized amplitude of an input frequency (of 10 Hz). (b)
Resultant of the background signal, defined as input signal in
(b) subtracted from raw signal in (a). (c) FFT of the resultant
signal in (c). Notice that small peaks are shown within between
the centered beam and the major peak around 0.1 Hz (around
1% of dark signal). Thus the modulation in a background noise
of a dynamical image correlation function can be used as addi-
tional information to characterize the dynamical states in much
weak signal-oriented physical systems.
domain is approximately averaged value of 20 μm, where
the dimensions of an individual fd-virus particle is about
~1 um long and its diameter is ~ 0.03 μm (for a low
ionic strength). Thus the transmitted intensity at a given
pixel, which is calibrated as ~ 0.96 μm/pixel conversion
rate, is calculated for the image correlation function, as
normalized with averaging over all pixels. The image
speckle patterns of the dynamical states are obtained
from the modified image sequence at a certain time step,
subtracted from the raw image, and image correlation
function with an initial intensity profile (at a time t = 0 s).
It turned out to be important to choose the initial frame
of the dynamical event as much as in quasi-equilibrium
process to carry on the meso-scopic relaxation dynamics.
For instance, we perform to recording after 3-5 min. of
waiting after applying field condition to ensure that more
or less the same dynamical events are occurred during
the measurement. Also depending on the characters of
the dynamical events, either slow or fast-process, the
time binning should be at least ~ 3-10 times faster for
extracting the appropriate time resolution. The resulting
correlation function in time of the speckles (or our small
nematic domains) is found to be as an exponential decay
function with a slightly stretched exponent. The mesoscopic
relaxation time of melting and forming of small nematic
domains is then obtained systematically by varying the
field amplitude at a low frequency. However, the mesos-
copic relaxation time constant in “fast” dynamical states
has tendency of saturation with the field amplitude at
higher field amplitude. This may be related to either the
lack of the resolution in our experiments, or the genuine
behavior in the dynamical system. Thus it would be
worthy to test the prediction of the relaxation time with
an image correlation spectroscopy for other similar dy-
namical events of biological interest or medical envi-
ronments.
Interestingly, we have also found that the time con-
stant for the relaxation behavior diverges on approaching
to the chiral-phase regime from the transition of dy-
namical states (close to ~ 3.2 V/mm). This may imply
the dynamical states are the consequence of the interac-
tion of charged fd-rods via induced polarization effect in
thick electric double-layers. We are currently developing
the possible theory for an explanation.
To conclude, mesoscopic time and length scale imag-
ing correlation spectroscopy needs a challenge to cus-
tomize the biomedical technology of soft-tissues. The
main difficulties are not only related to the density dif-
ferences, but also the “weak” and “reliability” of signals/
images of temporal evolution in micro-structural contrast.
However as far as any soft-organs or tissues consist of
fibrous structures in the presence of multivalent ionic
liquids, any types of the elastic envelops can be moni-
tored by their orientations in biological fluids. Then the
orientation contrast can be conveniently visualized by
depolarized light that can be combined with time-lapsed
632 K. Kang et al. / J. Biomedical Science and Engineering 3 (2010) 625-632
Copyright © 2010 SciRes.
imaging processing, as we have introduced in this report.
If one could come up with an integrated package for fast
time-lapsed imaging processing to pixel-to-pixel inten-
sity auto correlation signal progressing, then this could
be an application to characterize the mesoscopic relaxa-
tion behaviors of dynamical situations in many relevant
soft biological and biomedical front matters. It can be
adapted to early detection or “progressive” medicare is-
sues. For instance, biological fluids (as blood, saliva,
urine) can be accessed by in-vitro method under a low
external electric field to recognize the “abnormality”
related the growth/decay of “foreign” cells in terms of
the change of microscopic “viscosity” or “resistance”.
JBiSE
In this aspect, the “controllable” non-equilibrium ap-
proach of the dynamical image correlation spectroscopy
is much more informative than a steady-state or quasi-
equilibrium image processing.
5. ACKNOWLEDGEMENTS
Author thanks to J.K.G. Dhont for his scientific insight and introduce
the flexible-time resolving image correlation functions to characterize
the relaxation time in slow and fast dynamical states.
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