Repair of critical size rat calvarial defects using endometrial-derived stem cells embedded within gelatin/apatite nanocomposite scaffold

Jafar Ai, Saeed Heidari-Keshel, Mahmoud Azami, Armin Ai, Naghmeh Bahrami, Abodoreza Mohamadnia

Clinical Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Molecular Medicine, Hamedan University of Medical Sciences, Hamedan, Iran.
Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

DOI: 10.4236/scd.2013.31006                

Abstract

Human stromal stem cells derived from endometrium (hESCs) are a type of multipotent stromal cells of the proven ability to differentiate into osteogenic lineage. Thus, it was suggested that these cells may be used to repair skeletal defects. In this study, Human ESCs were extracted from female endometrium and harvested. Biomimetic gelatin/apatite (Gel/Ap) scaffolds with and without harvested cells were implanted in a Critical size calvarial defects in the cranial bone of adult male rat. To CT-Scan and Histological studies were performed to investigate the level of bone formation after 8 weeks of surgery. Results confirmed that the treated defects with the bare and hESCs grafted Gel/Ap scaffold showed significant bone formation and maturation in comparison with the control group.

Keywords

Biomimetic Scaffold; Bone Regeneration; Calvarial Defect; CT-Scan

Conflicts of Interest

The authors declare no conflicts of interest.

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