Author(s)

Yuxin Chen¹, Qigui Mo¹, Baibo Xie^2,3, Bingxin Ma¹, Xinyu Zang^2,3, Gao Zhou¹, Linyou Cheng³, James Hua Zhou^2,3*, Youwei Wang^1,4*

¹Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
²Beijing Hebabiz Biotechnology Co. Ltd., Beijing, China.
³National and Region joint Engineering Center for Anticancer Drug Development, Guangxi Hebabiz Pharmaceutical Co. Ltd., Qinzhou, China.
⁴MOE Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University, Wuhan, China.

Background: To explore the hepatoprotective effect of Yigan mingmu oral liquid (YGMM) on isoniazid-rifampicin induced liver injury in rats. Methods: Total 38 SD rats were randomly divided into 6 groups including control group, model group, silymarin positive control group, and three YGMM treatment groups. Model group was administered intragastrically with INH (100 mg/kg) and RIF (100 mg/kg) for 14 days. Silymarin group and YGMM treatment groups were administered intragastrically with silymarin (100 mg/kg) and different doses of YGMM (1, 2.5, 5 mg/kg) 2 hours before INH and RIF administration from day 4 to day 14. Results: Rats were sacrificed 16 hours after the last day treatment to determine the activities of serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), as well as total bilirubin (TB) content. Oxidative stress was evaluated by measuring total superoxide dismutase (T-SOD) and malondialdehyde (MDA) levels. Histopathological changes in liver tissues were observed under an optical microscope by using hematoxylin and eosin staining. The mice in model groups showed significantly (p < 0.05) increased levels in AST, ALT, ALP, TB and MDA compared to their control groups; and showed significantly (p < 0.05) decreased level in T-SOD. These changes were significantly (p < 0.05) reversed by the YGMM treatments in a dose-dependent manner. Hepatic pathological changes were attenuated or even reversed by silymarin or YGMM treatments. Conclusions: YGMM has a good hepatoprotective activity on isoniazid-rifampicin induced liver injuries in rats.

Herbal Prescription, Hepatoprotective Activity, Isoniazid, Rifampicin, Oxida-tive Stress

Chen, Y. , Mo, Q. , Xie, B. , Ma, B. , Zang, X. , Zhou, G. , Cheng, L. , Zhou, J. and Wang, Y. (2018) Hepatoprotective Activity of Yigan Mingmu Oral Liquid against Isoniazid/Rifampicin-Induced Liver Injuries in Rats. Chinese Medicine, 9, 165-178. doi: 10.4236/cm.2018.94010.