Rejuvenation and Adventitious Rooting in Coppice-Shoot Cuttings of Tectona grandis as Affected by Stock-Plant Etiolation

Azamal Husen

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DOI: 10.4236/ajps.2011.23042                

Abstract

The effect of stock-plant etiolation on adventitious rooting of single-node leafy cuttings (SNCs) made from coppice shoots from different age groups of donor plants were investigated for teak (Tectona grandis Linn. f.). When donor plants age were 1-, 2-, 3-, 4-, and 5-year-old, they were coppiced and maintained in the dark for etiolation, while a parallel set was kept in normal light in an open environment. After 20 days, coppice shoots were made into SNCs which were cultured under intermittent mist for rooting. Stock-plant etiolation significantly increased percent rooting, shoot and root length, and number of roots per SNC, but callusing at the base of SNCs decreased. Etiolated SNCs have shown 71.7% rooting, whereas for controls (non-etiolated), the response was 41.7%. The effect of aging on callusing, rooting and sprouting of etiolated and non-etiolated coppice shoots cuttings varied widely. Aging of donor plants decreased rooting and sprouting capability in SNCs and increased callusing at the base of etiolated cuttings. The results showed that stock-plant etiolation in 1-, 2-, 3-, 4-, and 5-year-old donor plants hastened rooting potential by rejuvenation of coppice shoots.

Keywords

Adventitious Rooting, Cuttings, Etiolation, Maturity, Tectona grandis

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	D. Pandey and C. Brown, “Teak: A Global Overview,” Unasylva, Vol. 51, No. 201, 2000. pp. 3-12.
[2]	D. N. Tewari, “A Monograph on Teak (Tectona grandis Linn. f.),” International Book Distributors, Dehra Dun, 1992.
[3]	E. P. Indira, S. Chand Basha and K. C. Chacko, “Effect of Seed Size Grading on the Germination and Growth of Teak (Tectona grandis) Seedling,” Journal of Tropical Forest Science, Vol. 12, No. 1, 2000, pp. 21-27.
[4]	M. Pal, “Vegetative Propagation of Teak by Rooting Stem Cuttings,” Proceeding of Second Forestry Conference, Vol. 2, 1992, pp.145-148.
[5]	W. P. Hackett and J. R. Murray, “Maturation and Rejuvenation in Woody Species,” In: M. R. Ahuja, Ed., Micropropagation of Woody Plants, Kluwer Academics Publishers, Dordrecht, 1993, pp. 93-105.
[6]	M. S. Greenwood, C. Díaz-sala, P. B. Singer, A. Decker and K. W. Hutchison, “Differential Gene Expression during Maturation-Caused Decline in Adventitious Rooting Ability in Loblolly Pine (Pinus taeda L.),” In: A. Altman and Y. Weisel, Eds., Biology of Adventitious Root Formation and Development, Plenum Press, New York, 1997, pp. 203-208. doi:10.1007/978-1-4615-5403-5_37
[7]	A. Husen and M. Pal, “Variation in Shoot Anatomy and Rooting Behaviour of Stem Cuttings in Relation to Age of Donor Plants in Teak (Tectona grandis Linn. f.),” New Forests, Vol. 31, No. 1, 2006, pp. 57-73. doi:10.1007/s11056-004-6794-5
[8]	A. Husen and M. Pal, “Metabolic Changes during Adventitious Root Primordium Development in Tectona grandis Linn. f. (Teak) Cuttings as Affected by Age of Donor Plants and Auxin (IBA and NAA) Treatment,” New Forests, Vol. 33, No. 3, 2007, pp. 309-323. doi:10.1007/s11056-006-9030-7
[9]	R. R. B. Leakey, “Capacity for Vegetative Propagation in Trees,” In: M. G. R. Cannel and J. Jackson, Eds., Attributes of Trees as Crop Plants, Institute of Terrestrial Ecology, Huntingdon, 1985, pp. 110-133.
[10]	A. Husen and M. Pal, “Effect of Serial Bud Grafting and Etiolation on Rejuvenation and Rooting Cuttings of Mature Trees of Tectona grandis Linn. f.,” Silvae Genetica, Vol. 52, No. 2, 2003, pp. 84-87.
[11]	A. Husen and M. Pal, “Clonal Propagation of Teak (Tectona grandis Linn. f.): Effect of IBA Application and Adventitious Root Regeneration on Vertically Split Cuttings,” Silvae Genetica, Vol. 52, No. 3-4, 2003, pp. 173-176.
[12]	A. Husen and M. Pal, “Seasonal Changes in Rooting Response of Hardwood Cuttings of Teak (Tectona grandis Linn. f.) in Relation to Drift of Total Soluble Sugar, Starch and Total Nitrogen,” Annals of Forestry, Vol. 15, 2007, pp. 11-31.
[13]	A. Husen and M. Pal, “Effect of Branch Position and Auxin Treatment on Clonal Propagation of Tectona grandis Linn. f,” New Forests, Vol. 34, No. 3, 2007, pp. 223-233. doi:10.1007/s11056-007-9050-y
[14]	J. Ludwig-Müller, A. Vertocnik and C. D. Town, “Analysis of Indole-3-Butyric Acid-Induced Adventitious Root Formation on Arabidopsis Stem Segments,” Journal of Experimental Botany, Vol. 56, No. 418, 2005, pp. 2095-2105. doi:10.1093/jxb/eri208
[15]	C. Sorin, D. B. John, I. Camus, K. Ljung, M. Kowalczyk, G. Geiss, H. Mckhann, C. Garcion, H. Vaucheret, G. Sandberg and C. Bellini, “Auxin and Light Control of Adventitious Rooting in Arabidopsis Require ARGONAUTE1,” Plant Cell, Vol. 17, No. 5, 2005, pp. 1-17. doi:10.1105/tpc.105.031625
[16]	A. Husen, “Clonal Propagation of Dalbergia sissoo Roxb. by Softwood Nodal Cuttings: Effects of Genotypes, Application of IBA and Position of Cuttings on Shoots,” Silvae Genetica, Vol. 53, 2004, pp. 50-55.
[17]	A. Husen, “Clonal Propagation of Dalbergia sissoo Roxb. and Associated Metabolic Changes during Adventitious Root Primordium Development,” New Forests, Vol. 36, No. 1, 2008, pp. 13-27. doi:10.1007/s11056-007-9079-y
[18]	A. Husen, “Stock-Plant Etiolation Causes Drifts in Total Soluble Sugars and Anthraquinones, and Promotes Adventitious Root Formation in Teak (Tectona grandis L. f.) Coppice Shoots,” Plant Growth Regulation, Vol. 54, No. 1, 2008, pp. 13-21. doi:10.1007/s10725-007-9222-y
[19]	B. K. Maynard and N. L. Bassuk, “Etiolation and Banding Effects on Adventitious Root Formation” In: T. D. Davis, B. E. Haissig and N. Sankhla, Eds., Adventitious Root Formation by Cuttings, Dioscorides Press, Portland, 1988, pp. 29-46.
[20]	B. K. Maynard and N. L. Bassuk, “Effect of Stock Plant Etiolation, Shading, Banding, and Shoot Development on Histology and Cutting Propagation of Carpinus betulus L Fastigata,” Journal of the American Society for Horticultural Science, Vol. 121, No. 5, 1996, pp. 853-860.
[21]	B. E. Haissig and T. D. Davis, “A Historical Evaluation of Adventitious Rooting Research to 1993,” In: T. D. Davis and B. E. Haissig, Eds., Biology of Adventitious Root Formation, Plenum Press, New York, 1994, pp. 275-331.
[22]	M. Pal, “Physiological and Biochemical Effects of Seasonal Changes in Stem Cuttings in Relation to Rooting,” PhD Dissertation, Punjab University, Chandigarh, 1978.
[23]	A. Husen, “Physiological Effects of Phytohormones and Mineral Nutrients on Adventitious Root Formation and Clonal Propagation of Tectona grandis Linn. f.,” PhD Dissertation, Forest Research Institute, Dehra Dun, 2002.
[24]	B. E. Haissig, “Reduced Irradiance and Applied Auxin Influence Carbohydrate Relation in Pinus banksiana Cuttings during Propagation,” Physiologia Plantarum, Vol. 78, No. 3, 1990, pp. 455-461. doi:10.1111/j.1399-3054.1990.tb09063.x
[25]	D. E. Brown, A. M. Rashotte, A. S. Murphy, J. Normanly, B. W. Tague, W. A. Peer, L, Taiz and G. K. Muday, “Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis,” Plant Physiology, Vol. 126, No. 2, 2001, pp. 524-535. doi:10.1104/pp.126.2.524
[26]	C. S. Buer and G. K. Muday, “The Transparent Testa4 Mutation Prevents Flavonoid Synthesis and Alters Auxin Transport and the Response of Arabidopsis Roots to Gravity and Light,” Plant Cell, Vol. 16, No. 5, 2004, pp. 1191-1205. doi:10.1105/tpc.020313
[27]	R. R. B. Leakey and R. Sstoreton-West, “The Rooting Ability of Triplochiton scleroxylon Cuttings: The Interactions between Stock Plant Irradiance, Light Quality and Nutrients,” Forest Ecology and Management, Vol. 49, No. 1-2, 1992, pp. 133-150. doi:10.1016/0378-1127(92)90166-7
[28]	C. Díaz-sala, G. Garrido and B. Sabater, “Age-Related Loss of Rooting Capability in Arabidopsis thaliana and Its Reversal by Peptides Containing the Arg-Gly-Asp (RGD) Motif,” Physiologia Plantarum, Vol. 114, No. 4, 2002, pp. 601-607. doi:10.1034/j.1399-3054.2002.1140414.x
[29]	G. Osterc, M. ?tefan?i?, and F. ?tampar, “Juvenile Stock Plant Material Enhances Root Development through Higher Endogenous Auxin Level,” Acta Physiologiae Plantarum, Vol. 31, No. 5, 2009, pp. 899-903. doi:10.1007/s11738-009-0303-6
[30]	E. Amri, H. V. B. Lyaruu, A. S. Nyomora and Z. L. Kanyelka, “Vegetative Propagation of African Blackwood (Dalbergia melanoxylon Guill. & Perr.): Effects of Age on Donor Plant, IBA Treatment and Cutting Position on Rooting Ability of Stem Cuttings,” New Forests, Vol. 39, No. 2, 2010, pp. 183-194. doi:10.1007/s11056-009-9163-6
[31]	M. S. Greenwood, X. Cui and F. Xu, “Response to Auxin Changes during Maturation-Related Loss of Adventitious Rooting Competence in Loblolly Pine (Pinus taeda) stem Cuttings,” Physiologia Plantarum, Vol. 111, No. 3, 2001, pp. 373-380. doi:10.1034/j.1399-3054.2001.1110315.x