Supplementation of Fructooligosaccharide Mildly Improves the Iron Status of Anemic Rats Fed a Low-Iron Diet

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DOI: 10.4236/fns.2017.82019    384 Downloads   481 Views  

ABSTRACT

Also known as a prebiotic, fructooligosaccharide (FOS) resists digestion by gastric acid and pancreatic enzymes in vivo, but is preferentially fermented by beneficial intestinal bacteria once it reaches the colon. While some studies suggest that FOS and its fermentation products may influence the iron absorption process, the effects of prolonged FOS supplementation on iron status remain unclear. The objective of this study was therefore to determine the enhancing effects of FOS supplementation on the iron status of anemic rats. Male Sprague-Dawley rats receiving a low-iron diet (12 μg/g) for 14 days showed significantly lower hemoglobin concentration, as well as lower tissue non-heme iron levels than rats receiving a regular diet (45 μg/g), confirming iron-deficiency anemia. On the first day of the feeding trial, two groups of anemic rats (n = 6) were fed the same low-iron diet with or without FOS supplementation, while two other groups of anemic rats were switched to the regular diet with or without FOS supplementation to allow recovery. FOS was provided to the rats by dissolving in water at 5% (w/v). Anemic rats fed the low-iron diet showed a mild increase (p < 0.05) in hemoglobin level after 21 days of FOS supplementation when compared to rats without FOS. For anemic rats switched to the regular diet, hemoglobin level returned to normal after 14 days and FOS supplementation showed no additional effects. Our results suggest that FOS supplementation has a mild enhancing effect on the iron status of anemic subjects on a low-iron diet.

Cite this paper

Zhang, F. , Lam Yung, K. , Sum Man Chung, S. and Yeung, C. (2017) Supplementation of Fructooligosaccharide Mildly Improves the Iron Status of Anemic Rats Fed a Low-Iron Diet. Food and Nutrition Sciences, 8, 294-304. doi: 10.4236/fns.2017.82019.

References

[1] Cummings, J.H., Macfarlane, G.T. and Englyst, H.N. (2001) Prebiotic Digestion and Fermentation. The American Journal of Clinical Nutrition, 73, 415s-420s.
[2] Langlands, S.J., Hopkins, M.J., Coleman, N. and Cummings, J.H. (2004) Prebiotic Carbohydrates Modify the Mucosa Associated Microflora of the Human Large Bowel. Gut, 53, 1610-1616.
https://doi.org/10.1136/gut.2003.037580
[3] Spiegel, J.E., Rose, R., Karabell, P., Frankos, V.H. and Schmitt, D.F. (1994) Safety and Benefits of Fructooligosaccharides as Food Ingredients. Food Technology, 48, 85-89.
[4] Jeurink, P.V., van Esch, B.C., Rijnierse, A., Garssen, J. and Knippels, L.M. (2013) Mechanisms Underlying Immune Effects of Dietary Oligosaccharides. The American Journal of Clinical Nutrition, 98, 572S-577S.
https://doi.org/10.3945/ajcn.112.038596
[5] Schmidt, K., Cowen, P.J., Harmer, C.J., Tzortzis, G., Errington, S. and Burnet, P.W. (2015) Prebiotic Intake Reduces the Waking Cortisol Response and Alters Emotional Bias in Healthy Volunteers. Psychopharmacology, 232, 1793-1801.
https://doi.org/10.1007/s00213-014-3810-0
[6] Gorski, D. (1997) Ingredient Forecast Product Development for World Markets. Dairy Foods, 98, 60-62.
[7] Lynch, S.R. (2011) Why Nutritional Iron Deficiency Persists as a Worldwide Problem. The Journal of Nutrition, 141, 763S-768S.
https://doi.org/10.3945/jn.110.130609
[8] Camaschella, C. (2015) Iron-Deficiency Anemia. The New England Journal of Medicine, 372, 1832-1843.
https://doi.org/10.1056/NEJMra1401038
[9] Yeung, C.K., Glahn, R.E., Welch, R.M. and Miller, D.D. (2005) Prebiotics and Iron Bioavailability—Is There a Connection? Journal of Food Science, 70, R88-R92.
https://doi.org/10.1111/j.1365-2621.2005.tb09984.x
[10] Patterson, J.K., Rutzke, M.A., Fubini, S.L., Glahn, R.P., Welch, R.M., Lei, X. and Miller, D.D. (2009) Dietary Inulin Supplementation Does Not Promote Colonic Iron Absorption in a Porcine Model. Journal of Agricultural and Food Chemistry, 57, 5250-5256.
https://doi.org/10.1021/jf900698x
[11] Tako, E., Glahn, R.P., Welch, R.M., Lei, X., Yasuda, K. and Miller, D.D. (2008) Dietary Inulin Affects the Expression of Intestinal Enterocyte Iron Transporters, Receptors and Storage Protein and Alters the Microbiota in the Pig Intestine. British Journal of Nutrition, 99, 472-480.
https://doi.org/10.1017/S0007114507825128
[12] Samolińska, W. and Grela, E.R. (2017) Comparative Effects of Inulin with Different Polymerization Degrees on Growth Performance, Blood Trace Minerals, and Erythrocyte Indices in Growing-Finishing Pigs. Biological Trace Element Research, 176, 130-142.
[13] Sazawal, S., Dhingra, U., Hiremath, G., Sarkar, A., Dhingra, P., Dutta, A., Menon, V.P. and Black, R.E. (2010) Effects of Bifidobacterium lactis HN019 and Prebiotic Oligosaccharide Added to Milk on Iron Status, Anemia, and Growth among Children 1 to 4 Years Old. Journal of Pediatric Gastroenterology and Nutrition, 51, 341-346.
[14] Coudray, C., Bellanger, J., Castiglia-Delav, C., Remesy, C., Vermorel, M. and Rayssignuier, Y. (1997) Effect of Soluble or Partly Soluble Dietary Fibres Supplementation on Absorption and Balance of Calcium, Magnesium, Iron and Zinc in Healthy Young Men. European Journal of Clinical Nutrition, 51, 375-380.
https://doi.org/10.1038/sj.ejcn.1600417
[15] Van den Heuvel, E.G., Schaafsma, G., Muys, T. and van Dokkum, W. (1998) Nondigestible Oligosaccharides Do Not Interfere with Calcium and Nonheme-Iron Absorption in Young, Healthy Men. The American Journal of Clinical Nutrition, 67, 445-451.
[16] Rebouche, C.J., Wilcox, C.L. and Widness, J.A. (2004) Microanalysis of Non-Heme Iron in Animal Tissues. Journal of Biochemical and Biophysical Methods, 58, 239-251.
https://doi.org/10.1016/j.jbbm.2003.11.003
[17] Ha, J.H., Doguer, C., Wang, X., Flores, S.R. and Collins, J.F. (2016) High-Iron Consumption Impairs Growth and Causes Copper-Deficiency Anemia in Weanling Sprague-Dawley Rats. PLoS ONE, 11, e0161033.
https://doi.org/10.1371/journal.pone.0161033
[18] Felt, B.T., Beard, J.L., Schallert, T., Shao, J., Aldridge, J.W., Connor, J.R., Georgieff, M.K. and Lozoff, B. (2006) Persistent Neurochemical and Behavioral Abnormalities in Adulthood Despite Early Iron Supplementation for Perinatal Iron-Deficiency Anemia in Rats. Behavioural Brain Research, 171, 261-270.
https://doi.org/10.1016/j.bbr.2006.04.001
[19] National research Council. (1995) Nutrient Requirements of Laboratory Animals: 1995. National Academies Press, Washington DC.
[20] Moshfegh, A.J., Friday, J.E., Goldman, J.P. and Ahuja, J.K.C. (1999) Presence of Inulin and Oligofructose in the Diets of Americans. The Journal of Nutrition, 129, 1407S-1411S.
[21] Coussement, P.A.A. (1999) Inulin and Oligofructose: Safe Intakes and Legal Status. The Journal of Nutrition, 129, 1412S-1417S.
[22] Mejia, L.A., Hodges, R.E. and Rucker, R.B. (1979) Role of Vitamin A in the Absorption, Retention and Distribution of Iron in the Rat. The Journal of Nutrition, 109, 129-137.
[23] Andrews, N.C. (1999) Disorders of Iron Metabolism. The New England Journal of Medicine, 341, 1986-1995.
https://doi.org/10.1056/NEJM199912233412607
[24] Stewart, M.L., Timm, D.A. and Slavin, J.L. (2008) Fructooligosaccharides Exhibit More Rapid Fermentation than Long-Chain Inulin in an in Vitro Fermentation System. Nutrition Research, 28, 329-334.
https://doi.org/10.1016/j.nutres.2008.02.014
[25] Shiga, K., Nishimukai, M., Tomita, F. and Hara, H. (2006) Ingestion of Difructose Anhydride III, a Non-Digestible Disaccharide, Prevents Gastrectomy-Induced Iron Malabsorption and Anemia in Rats. Nutrition, 22, 786-793.
https://doi.org/10.1016/j.nut.2005.12.013
[26] Suzuki, T., Nishimukai, M., Shinoki, A., Taguchi, H., Fukiya, S., Yokota, A., Saburi, W., Yamamoto, T., Hara, H. and Matsui, H. (2010) Ingestion of Epilactose, a Non-Digestible Disaccharide, Improves Postgastrectomy Osteopenia and Anemia in Rats through the Promotion of Intestinal Calcium and Iron Absorption. Journal of Agricultural and Food Chemistry, 58, 10787-10792.
https://doi.org/10.1021/jf102563y
[27] Bougle, D., Vaghefi-Vaezzadeh, N., Roland, N., Bouvard, G., Arhan, P., Bureau, F., Neuville, D. and Maubois, J.L. (2002) Influence of Short-Chain Fatty Acids on Iron Absorption by Proximal Colon. Scandinavian Journal of Gastroenterology, 37, 1008-1011.
https://doi.org/10.1080/003655202320378176
[28] Sakai, K., Ohta, A., Shiga, K., Takasaki, M., Tokunaga, T. and Hara, H. (2000) The Cecum and Dietary Short-Chain Fructooligosaccharides Are Involved in Preventing Postgastrectomy Anemia in Rats. The Journal of Nutrition, 130, 1608-1612.
[29] Collings, R., Harvey, L.J., Hooper, L., Hurst, R., Brown, T.J., Ansett, J., King, M. and Fairweather-Tait, S.J. (2013) The Absorption of Iron from Whole Diets: A Systematic Review. The American Journal of Clinical Nutrition, 98, 65-81.
https://doi.org/10.3945/ajcn.112.050609
[30] Duar, R.M., Ang, P.T., Hoffman, M., Wehling, R., Hutkins, R. and Schlegel, V. (2015) Processing Effects on four Prebiotic Carbohydrates Supplemented in an Extruded Cereal and a Low pH Drink. Cogent Food & Agriculture, 1, Article ID: 1013782.
https://doi.org/10.1080/23311932.2015.1013782
[31] Vega, R. and Zuniga-Hansen, M.E. (2015) The Effect of Processing Conditions on the Stability of Fructooligosaccharides in Acidic Food Products. Food Chemistry, 173, 784-789.
https://doi.org/10.1016/j.foodchem.2014.10.119

  
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