Correlations between delayed fluorescence of chlorophyll, metabolism and yield of plants. II. Influence of moisture of leaf and temperature condition on delayed fluorescence of leaves

Armen B. Avagyan

Research and Industry Center of Photosynthesising Organisms, Fееd Additives and Physiologically Active Compounds, Yerevan, Armenia.

DOI: 10.4236/jbpc.2010.11006                

Abstract

During various temperatures of incubation the dehydration of leaves up to 3.2-3.8% mainly in-duced increase maximum amplitude of delayed fluorescence of chlorophyll. It was shown that moisture loss with this range could be deter-mined for the most part by the growth of the electrochemical potential of thylakoid mem-branes. The further incubation of detached leaves at 36℃ temperature, with more notable moisture loss, resulted in specific its decline as opposed to cases of 22 and 6℃ of thermal in-cubation. It was confirmed that the increased temperatures and moisture loss damage of the cells of plants occurred together induce a greater influence on plants than in case of oc-curring apart. The results allow to suppose that this can be mostly caused weakly associated polypeptides fallen out from the chloroplast membrane, which may be stipulated by high temperature combined with change ionic and osmotic stresses due moisture loss. Simulta-neously, the results showed that the exposure of the critical lowered air temperature led to considerable typical changes of leaves delayed fluorescence parameters of field plants. There-fore, their use can constitute new approaches to elucidate the molecular basis of plant freezing tolerance in a timely manner, based on concen-tration-related changes and the efficiency of coupling between light and dark processes of plants.

Keywords

Delayed Fluorescence of Chlorophyll; Temperature; Moisture; Frost; Weakly Associated Polypeptides

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Niculina, G. N. and Semichatova, O. A. (1983) Influence of a heat on the contents of adenylate in leaves of peas. The influence of a high temperature on the contents of the adenylates in pea leaves. Russian Plant Physiology, 30, 998-1005.
[2]	Tezara, W., Mitchell, V., Driscoll, S. P. and Lawlor, D. W. (2002) Effects of water deficit and its interaction with CO2 supply on the biochemistry and physiology of pho-tosynthesis in sunflower. Journal of Experimental Botany, 53, 1781-1791.
[3]	Polimbetova, F. A., Mamonov, L. K. and Kim, G. G. (1974) Estimation of cereal crops to drought and heat re-sistance in field conditions. Russian Agricultural Biology, 9, 235-237.
[4]	Nicolas, M. E., Gleadow, R. M. and Dalling, M. J. (1984) Effects of drought and high temperature on grain growth in wheat. Australian Journal of Plant Physiology, 11, 553-566.
[5]	Liu, W., Yuan, S., Zhang, N., Lei, T., Duan, H., Liang, H. and Lin, H. (2006) Effect of water stress on Photosystem 2 in two wheat cultivars. Biologia Plantarum, 50, 597- 602.
[6]	Ueda, A., Kathiresan, A., Inada, M., Narita, Y., Nakamura, T., Shi, W., Takabe, T. and Bennett, J. (2004) Osmotic stress in barley regulates expression of a different set of genes than salt stress does. Journal of Experimental Bo-tany, 55, 2213-2218.
[7]	Lu, C. and Zhang, J. (1999) Effects of water stress on Photosystem II photochemistry and its thermostability in wheat plants. Journal of Experimental Botany, 50, 1199- 1206.
[8]	Havaux, M. (1992) Stress tolerance of Photosystem II in vivo. Antagonistic effects of water, heat, and photoinhi-bition stresses. Plant Physiology, 100, 424-443.
[9]	Mar, T., Brehlner, J., Roy, G. (1975) Induction kinetics of delayed light emission in spinach chloroplasts. Biochimica et Biophysica Acta, 376, 345-353.
[10]	Avagyan, A. B. (2010) Correlations between Delayed Fluorescence of Chlorophyll, Metabolism and Yield of Plants. I. Influence of Fertilizers on Correlations. Journal of Biophysical Chemistry, 1. (in press.
[11]	Wraight, C. A. and Crofts, A. R. (1971) Delayed fluores-cence and the high-energy state of chloroplast. European Journal of Biochemistry, 19, 386-397.
[12]	Mar, T., Brehlner, J. and Roy, G. (1975) Induction kinetics of delayed light emission in spinach chloroplasts. Bi-ochimica et Biophysica Acta, 376, 345-353.
[13]	Goltsev, V., Zaharieva, I., Lambrev, P., Yordanov, I. andStrasser, R. (2003) Simultaneous analysis of prompt and delayed chlorophyll a fluorescence in leaves during the induction period of dark to light adaptation. Journal of Theoretical Biology, 225, 171-183.
[14]	Goltsev, V., Chernev, P., Zaharieva, I., Lambrev, P., Strasser, R. J. (2005) Kinetics of delayed chlorophyll a fluorescence registered in milliseconds time range. Pho-tosynthesis Research, 84, 209-215.
[15]	Avagyan, A. B. (1986) Effect of air temperature and soil moisture content on delayed fluorescence in Pisum sati-vum leaves in field conditions. Russian Fiziologiya Ras-tenii, 33, 23-28. http://cat.inist.fr/?aModele = afficheNcpsidt = 876468.
[16]	Berry, J. A and Downton, W. J. S. (1987) Photosynthesis dependence from influence of environment factors. Pho-tosynthesis, 2, 276-354.
[17]	Korovin, A. I. (1984) Plants and extreme temperatures. Gidrometizdat, Sankt Petersburg.
[18]	Avagyan, A. B., Venedictov, P. S., Dobrecov, G. E. and Rubin, A. B. (1982) Influence heat damage of pea chlo-roplasts on their interaction with fluorescent probe ANS. Biological Sciences, 11, 30-35.
[19]	Muller, A., Witt, H. T. (1961) Trapped primary product of photosynthesis in green plants. Nature, 189, 944-945.
[20]	Kafalieva, D. N. and Bushuev, M. C. (1979) Influence of temperature on photoinduced proton gradient in isolated chloroplasts. Russian Biophysics, 24, 676-680.
[21]	Avagyan, A. B., Venedictov, P. S. and Rubin, A. B. (1982) Interaction between fluorescent probe ANS and chlorop-lasts. Russian Biophysics, 27, 415-419.
[22]	Avagyan, A. B., Venedictov, P. S., Rubin, A. B. (1984) Application of rodamin 6g as a fluorescent probe for study of chloroplast membranes. Russian Biophysics, 29, 980-983.
[23]	Avagyan, A. B. (1987) The application of the method of fluorescent probes in research of chloroplast membrane properties. Biological Journal of Armenia, 40, 443-448.
[24]	Becker, D. W., Callahan, F. E. and Chiniae, G. M. (1985) Photoactivation of NH2OH-treated leaves: reassembly of realized extrinsic PSII polypeptides and relegation of Mn catalyst of water oxidation. FEBS Letters, 192, 209-214.
[25]	Avagyan, A. B., Venedictov, P. S., Dobrecov, G. E., Rubin, A. B. (1983) Influence uni- and bivalence cations on in-teraction fluorescent probe ANS and rodamin 6g with chloroplasts. Biological Sciences, 5, 33-36.
[26]	Zaharieva, I. and Goltsev, V. (2003) Advances on Photo-system II investigation by measurement of delayed chlo-rophyll fluorescence by a phosphoroscopic method. Pho- tochemistry and Photobiology, 77, 292-298.
[27]	Neuner, G. and Pramsohler, M. (2006) Freezing and high temperature thresholds of Photosystem II compared to ice nucleation, frost and heat damage in evergreen subalpine plants. Physiologia Plantarum, 126, 196-204.
[28]	Yi, X. F. and Zhang, Z. B. (2008) Influence of in-sect-infested cotyledons on early seedling growth of Mongolian oak, Quercus mongolica. Photosynthetica, 46, 139-142.
[29]	Ehlert, B. and Hincha, D. K. (2008) Chlorophyll fluores-cence imaging accurately quantifies freezing damage and cold acclimation responses in Arabidopsis leaves. Plant Methods. www.plantmethods.com/content/4/1/12.