Evaluation of the Behaviour of Wrinkles Fibroblasts and Normal Aged Fibroblasts in the Presence of Poly-L-Lactic Acid

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DOI: 10.4236/jcdsa.2012.21006    3,536 Downloads   7,016 Views   Citations


Background: Wrinkles are characterized by changes in the organization and structure of the dermis. Human wrinkle fibroblasts (WF) have a different functional behaviour in comparison with normal-aged fibroblasts (NF). Decreases in migration capacities and collagen I synthesis are observed. Mitochondrial function is impaired with an increase in lactate production during aging. Sculptra? (poly-L-lactic acid: PLLA), a biodegradable synthetic polymer, is used for subcutaneous volume restoration. Thus we decided to investigate different fibroblast functions when placed in contact with PLLA. Objectives: The potential of PLLA to compensate for the reduction of metabolic activity, to restore the migration capacity of WF and to inhibit the lactate production, was investigated and compared to NF. Methods: Two different skin samples were used from each of the three women’s facelift (one inside a face wrinkle and one from normal aged skin). Collagen I, lactate productions and proliferation capacities were investigated on monolayer cultures. Migration properties were evaluated using three-dimensional collagen lattices. Results: PLLA increased collagen I synthesis, restored migration capacities and tended to decrease lactate production in WF, whereas PPLA stimulated proliferation in NF and tended to improve the migration of NF. Conclusion: These results suggested that PLLA from Sculptra? acted as a stimulus for collagen production in WF and that it is suitable for correcting skin depressions, such as wrinkles.

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C. Courderot-Masuyer, S. Robin, H. Tauzin and P. Humbert, "Evaluation of the Behaviour of Wrinkles Fibroblasts and Normal Aged Fibroblasts in the Presence of Poly-L-Lactic Acid," Journal of Cosmetics, Dermatological Sciences and Applications, Vol. 2 No. 1, 2012, pp. 20-27. doi: 10.4236/jcdsa.2012.21006.


[1] C. M. Burgess and R. M. Quiroga, “Assessment of the Safety and Efficacy of Poly-L-Lactic Acid for the Treatment of HIV-Associated Facial Lipoatrophy,” Journal of the American Academy of Dermatolology, Vol. 52, No. 2, 2005, pp. 233-239. doi:10.1016/j.jaad.2004.08.056
[2] R. Fitzgerald and D. Vleggaar, “Using Poly-L-Lactic Acid (PLLA) to Mimic Volume in Multiple Tissue Layers,” Journal of Drugs in Dermatology, Vol. 8, No. 10, 2009, pp. s5-s14.
[3] D. Vleggaar and U. Bauer, “Facial Enhancement and the European Experience with Sculptra (Poly-L-Lactic Acid),” Journal of Drugs in Dermatolology, Vol. 3, No. 5, 2004, pp. 542-547.
[4] J. K. Wildemore and D. H. Jones, “Persistent Granulomatous Inflammatory Response Injected by Injectable Poly-L-Lactic Acid for HIV Lipoatrophy,” Dermatologic Surgery, Vol. 32, No. 11, 2006, pp. 1407-1409. doi:10.1111/j.1524-4725.2006.32313.x
[5] M. Jouandeaud, C. Viennet, S. Bordes, B. Closs and P. Humbert, “Comparison of the Biomechanical and Biosynthetic Behavior of Normal Human Fibroblasts and Fibroblasts from Forehead Wrinkle,” IFSCC Magazine, Vol. 7, No. 2, 2004, pp. 2-6.
[6] J. Varani, M. K. Dame, L. Rittie L, S. E. Fligiel, S. Kang, G. J. Fisher and J. J. Voorhees, “Decreased Collagen Production in Chronologically Aged Skin: Roles of Age-Dependent Alteration in Fibroblast Function and Defective Mechanical Stimulation,” American Journal of Pathology, Vol. 168, No. 6, 2006, pp. 1861-1868. doi:10.2353/ajpath.2006.051302
[7] Y. H. Wei, S. B. Wu, Y. S. Ma and H. C. Lee, “Respiratory Function Decline and DNA Mutation in Mitochondria, Oxidative Stress and Altered Gene Expression during Aging,” Chang Gung Medical Journal, Vol. 32, No. 2, 2009, pp. 113-132.
[8] W. Zwerschke, S. Mazurek, P. Stockl P, E. Hutter, E. Eigenbrodt and P. Jansen-Durr, “Metabolic Analysis of Senescent Human Fibroblasts Reveals a role for AMP in Cellular Senescence,” The Biochemical Journal, Vol. 376, No. 2, 2003, pp. 403-411. doi:10.1042/BJ20030816
[9] E. Bell, B. Ivarsson and C. Merill, “Production of a Tissue-Like Structure by Contraction of Collagen Lattices by Fibroblasts of Different Proliferative Potential in Vitro,” Proceedings of the National Academy of Sciences of the USA, Vol. 76, No. 3, 1979, pp. 1274-1278. doi:10.1073/pnas.76.3.1274
[10] R. M. Lavker, P. S. Zheng and G. Dong, “Morphology of Aged Skin,” Clinics in Geriatric Medicine, Vol. 5, No. 1, 1989, pp. 53-67.
[11] J. Uitto, “Connective Tissue Biochemistry of the Aging Dermis. Age-Associated Alteractions in Collagen and Elastin,” Clinics in Geriatric Medicine, Vol. 5, No. 1, 1989, pp. 127-147.
[12] J. Varani, G. J. Fisher, S. Kang and J. J. Voorhees, “Molecular Mechanisms of Intrinsic Skin Aging and Retinoid-Induced Repair and Reversal,” Journal of Investigative Dermatolology. Symposium Proceedings, Vol. 3, No. 1, 1998, pp. 57-60.
[13] B. Woerle, C. W. Hanke and G. Sattler, “Poly-L-Lactic- Acid: A Temporary Filler for Soft Tissue Augmentation,” Journal of Drugs in Dermatology, Vol. 3, No. 4, 2004, pp. 385-389.
[14] B. L. Eppley and B. Dadvand, “Injectable Soft-Tisue Fillers: Clinical Overview,” Plastic and Reconstructive Surgery, Vol. 118, No. 4, 2006, pp. 98e-106e. doi:10.1097/01.prs.0000232436.91409.30
[15] R. N. Sherman, “Sculptra: The New Three-Dimensional Filler,” Clinics in Plastic Surgery, Vol. 33, No. 4, 2006, pp. 539-550. doi:10.1016/j.cps.2006.09.001
[16] S. M. Lam, B. Azizzadeh and M. Graivier, “Injectable Poly-L-Lactic Acid (Sculptra): Technical Considerations in Soft-Tissue Contourning,” Plastic and Reconstructive Surgery, Vol. 118, No. 3, 2006, pp. 55S-63S. doi:10.1097/01.prs.0000234612.20611.5a
[17] S. Gogolewski, M. Jovanovic, S. M. Perren, J. G. Dillon and M. K. Hughes, “Tissue Response and in Vivo Degradation of Selected Polyhydroxyacids: Polylactides (PLA), Poly(3-hydrroxubutarate) (PHB), and Poly(3-hydroxybu- tarate-co-3-hydroxyvalearte) (PHB/VA),” Journal of Bio- medical Material Research, Vol. 27, No. 9, 1993, pp. 1135-1148. doi:10.1002/jbm.820270904
[18] D. Vleggaar, “Facial Volumetric Correction with Injectable Poly-L-Lactic Acid,” Dermatologic Surgery, Vol. 31, No. 11 Pt 2, 2005, pp. 1511-1518.
[19] P. Engelhard, G. Humble and D. Mest, “Safety of Sculptra: A Review of Clinical Data,” Journal of Cosmetic and Laser Therapy, Vol. 7, No. 3/4, 2005, pp. 201-205. doi:10.1080/14764170500451404
[20] T. Lombardi, J. Samson, F. Plantier, C. Husson and R. Küffer, “Orofacial Granulomas after Injection of Cosmetic Fillers. Histopathological and Clinical Study of 11 Cases,” Journal of Oral Pathology & Medicine, Vol. 33, No. 2, 2004, pp. 115-120. doi:10.1111/j.1600-0714.2004.00194.x
[21] J. C. Dallon and H. P. Ehrlich, “A Review of Fibroblast- Populated Collagen Lattices,” Wound Repair and Regeneration, Vol. 16, No. 4, 2008, pp. 472-479. doi:10.1111/j.1524-475X.2008.00392.x
[22] S. Prahl, T. Kueper, T. Biernoth, Y. W?hrmann, A. Munster, M. Fürstenau, et al, “Aging Skin Is Functionally Anaerobic: Importance of Coenzyme Q10 for Anti Aging Skin Care,” BioFactors, Vol. 32, No. 1-4, 2008, pp. 245- 255. doi:10.1002/biof.5520320129
[23] H. Levinson, K. E. Koyer, G. C. Saggers and H. P. Ehrlich, “Calmodulin-Myosin Light Chain Kinase Inhibition Changes Fibroblast-Populated Collagen Lattice Contraction, Cell Migration, Focal Adhesion Formation, and Wound Contraction,” Wound Repair and Regeneration, Vol. 12, No. 5, 2004, pp. 505-511. doi:10.1111/j.1067-1927.2004.012502.x
[24] R. S. Adelstein, M. D. Pato, J.R. Sellers, P. de Lanerolle and M. A. Conti, “Regulation of Contractile Proteins by Reversible Phosphorylation of Myosin and Myosin Kinase,” Society of General Physiologists Series, Vol. 37, 1982, pp. 273-281.
[25] A. B. Lansdown, “Calcium: A Potential Central Regulator in Wound Healing in the Skin,” Wound Repair and Regeneration, Vol. 10, No. 5, 2002, pp. 271-285. doi:10.1046/j.1524-475X.2002.10502.x
[26] P. Carinci, M. Bodo, R. Evangelisti and E. Becchetti, “Involvement of the Cytoskeleton in Regulation of Collagen Synthesis and Secretion,” Italian Journal of Anatomy and Embryology, Vol. 100, Suppl. 1, 1995, pp. 65- 74.
[27] S. K. Donaldson, L. Hermansen and L. Bolles, “Differential, Direct Effects of H+ on Ca2+-Activated Force of Skinned Fibres from the Soleus, Cardiac and Adductor Magnus Muscles of Rabbits,” Pflugers Archive, Vol. 376, No. 1, 1978, pp. 55-65. doi:10.1007/BF00585248
[28] N. J. Kruse and P. Bornstein, “The Metabolic Requirements for Transcellular Movement and Secretion of Collagen,” The Journal of Biological Chemistry, Vol. 250, No. 13, 1975, pp. 4841-4847.
[29] K. R. Beer and M. I. Rendon, “Use of Sculptra Mark in Esthetic Rejuvenation,” Seminars in Cutaneous Medicine and Surgery, Vol. 25, No. 3, 2006, pp. 127-131. doi:10.1016/j.sder.2006.06.004

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