Simultaneous Determination of Catechins and Caffeine in Green Tea-Based Beverages and Foods for Specified Health Uses

Download Download as PDF (Size:1584KB)  HTML   XML  PP. 316-325  
DOI: 10.4236/fns.2017.83021    443 Downloads   607 Views  


Catechins in green tea have various useful features including antioxidant activity and preventive effects on metabolic syndrome. Various beverages that are enriched with tea catechins are marketed as Foods for Specified Health Uses (FOSHU) in Japan. However, recent reports have indicated that excessive consumption of green tea extracts as a dietary supplement are associated with adverse health effects such as liver disorders. Various catechins and caffeine are constituents of FOSHU tea-based beverages. The amount of catechins in FOSHU products is displayed on labels as total catechin content, but the content of individual catechins are not provided. Although health hazards of FOSHU products have rarely been reported, precise information about the content and types of catechins in FOSHU products is needed to ensure safety. We used high-performance liquid chromatography with a photodiode array (HPLC/PDA) to simultaneously identify and quantify catechins and caffeine in green tea-based popular beverages and FOSHU beverages. This technique allowed simultaneous quantitation of five types of catechins and caffeine in green tea without complicated sample preparation. Epigallocatechin gallate (EGCG) and epigallocatechin EGC were the main catechins in various FOSHU beverages and the concentrations of almost all catechins were higher in FOSHU, than in popular green tea-based beverages. The concentrations of EGCG in green tea-based popular beverages and in FOSHU beverages were 5.4 - 7.3 and 10.2 - 41.9 mg/100mL, respectively, with the highest concentration being in a product named Healthya (approximately 147 mg/bottle). The simultaneous determination of compounds such as catechins and caffeine in FOSHU beverages can help to estimate beneficial and adverse effects to prevent deleterious effects on health and the excessive consumption of FOSHU beverages containing high concentrations of tea catechins should be avoided.

Cite this paper

Maruyama, K. , Kihara-Negishi, F. , Ohkura, N. , Nakamura, Y. , Nasui, M. and Saito, M. (2017) Simultaneous Determination of Catechins and Caffeine in Green Tea-Based Beverages and Foods for Specified Health Uses. Food and Nutrition Sciences, 8, 316-325. doi: 10.4236/fns.2017.83021.


[1] Lambert, J.D. and Elias, R.J. (2010) The Antioxidant and Pro-Oxidant Activities of Green Tea Polyphenols: A Role in Cancer Prevention. Archives of Biochemistry and Biophysics, 501, 65-72.
[2] Yang, C.S., Lambert, J.D. and Sang, S. (2009) Antioxidative and Anti-Carcinogenic Activities of Tea Polyphenols. Archives of Toxicology, 83, 11-21.
[3] Ota, N., Soga, S., Shimotoyodome, A., Haramizu, S., Inaba, M., Murase, T. and Tokimitsu, I. (2005) Effects of Combination of Rgulation Exercise and Tea Catechins Intake on Energy Expenditure in Humans. Journal of Health Science, 51, 233-236.
[4] Kao, Y.H., Chang, H.H., Lee, M.J. and Chen, C.L. Mol. (2006) Tea, Obesity, and Diabetes. Molecular Nutrition & Food Research, 50, 188-210.
[5] Basu, A., Sanchez, K., Leyva, M.J., Wu, M., Betts, N.M., Aston, C.E. and Lyons, T.J. (2010) Green Tea Supplementation Affects Body Weight, Lipids, and Lipid Peroxidation in Obese Subjects with Metabolic Syndrome. Journal of the American College of Nutrition, 29, 31-40.
[6] Molinari, M., Watt, K.D., Kruszyna, T., Nelson, R., Walsh, M., Huang, W.Y., Nashan, B. and Peltekian, K. (2006) Acute Liver Failure Induced by Green Tea Extracts: Case Report and Review of the Litreature. Liver Transplantaion, 12, 1892-1895.
[7] Lambert, J.D., Kennett, M.J., Sang, S., Reuhl, K.R., Ju, J. and Yang, C.S. (2010) Hepatotoxicity of High Oral Dose (-)-Epigallocatechin-3-Gallate in Mice. Food and Chemical Toxicology, 48, 409-416.
[8] Kobayashi, M., Nishizawa, M., Inoue, N., Hosoya, T., Yoshida, M., Ukawa, Y., Sagesaka, Y.M., Doi, T., Nakayama, T., Kumazawa, S. and Ikeda, I. (2014) Epigallocatechin Gallate Decreases the Micellar Solubility of Cholesterol via Specific Interaction with Phosphatidylcholine. Journal of Agricultral and Food Chemistry, 62, 2881-2890.
[9] Goto, T., Saito, Y., Morikawa, K., Kanamaru, Y. and Nagaoka, S. (2012) Epigallocatechin Gallate Changes mRNA Expression Level of Genes Involved in Cholesterol Metabolism in Hepatocytes. British Journal of Nutrition, 107, 769-773.
[10] Miura, Y., Chiba, T., Miura, S., Tomita, I., Umegaki, K., Ikeda, M. and Tomita, T. (2000) Green Tea Polyphenols (Flavan 3-Ols) Prevent Oxidative Modification of Low Density Lipoproteins: An ex Vivo Study in Humans. Journal of Nutrition and Biochemistry, 11, 216-222.
[11] Sayama, K., Lin, S., Zheng, G. and Oguni, I. (2000) Effects of Green Tea on Growth, Food Utilization and Lipid Metabolism in Mice. In Vivo, 14, 481-484.
[12] Sugiura, C., Nishimatsu, S., Moriyama, T., Ozasa, S., Kawada, T. and Sayama, K. (2012) Catechins and Caffeine Inhibit Fat Accumulation in Mice through the Improvement of Hepatic Lipid Metabolism. Journal of Obesity, 2012, Article ID: 520510.
[13] Mazzanti, G., Menniti-Ippolito, F., Moro, P.A., Cassetti, F., Raschetti, R. and Santuccio, C. and Mastrangelo, S. (2009) Hepatotoxicity from Green Tea: A Review of the Literature and Two Unpublished Cases. European Journal of Clinical Pharmacology, 65, 331-341.
[14] Weinstein, D.H., Twaddell, W.S., Raufman, J.P., Philosophe, B. and Mindikoglu, A.L. (2012) SlimQuick™—Associated Hepatotoxicity in a Woman with Alpha-1 Antitrypsin Heterozygosity. World Journal of Hepatology, 4, 154-157.
[15] Patel, S.S., Beer, S., Kearney, D.L., Phillips, G. and Carter, B.A. (2013) Green Tea Extract: A Potential Cause of Acute Liver Failure. World Journal of Gastroenterology, 19, 5174-5177.

comments powered by Disqus

Copyright © 2017 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.