Effect of Combining Ultrasound and Mild Heat Treatment on Physicochemical, Nutritional Quality and Microbiological Properties of Pineapple Juice

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DOI: 10.4236/fns.2017.82015    532 Downloads   679 Views  


Increasing consumer awareness regarding the health benefits of different nutrients in food has led to the requirement of assessing the effect of food processing approaches on the quality attributes. The present work focuses on understanding the effects of ultrasound (US) processing, mild heat pasteurization (65°C for 15 min), thermal pasteurization (80°C for 15 min) and their combination on physicochemical, microbiological properties and nutritional quality of pineapple juice through 60 days of storage at room temperature. Ultrasound treatment showed significantly lower browning degree. Ultrasound followed by ultrasound combined with mild heat pasteurization (UMP) treatments was effective in retaining the total phenolic content of pineapple juice as compared to the thermal treatment or the untreated juice sample at room temperature during 60 days of storage. Thermal pasteurization (TP) followed by ultrasound combined with mild heat pasteurization (UMP) and ultrasound (US) treatment, in increasing order, was found to be effective in delaying microbial growth in pineapple juice. This study demonstrates that ultrasound combined with mild heat pasteurization treatments could be able to effectively inactivate the microorganisms and pectin methylesterase in pineapple juice whilst preserving relatively high amount of phenols.

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Lagnika, C. , Adjovi, Y. , Lagnika, L. , Gogohounga, F. , Do-Sacramento, O. , Koulony, R. and Sanni, A. (2017) Effect of Combining Ultrasound and Mild Heat Treatment on Physicochemical, Nutritional Quality and Microbiological Properties of Pineapple Juice. Food and Nutrition Sciences, 8, 227-241. doi: 10.4236/fns.2017.82015.


[1] Cao, G., Sofic, E. and Prior, R.L. (1996) Antioxidant Capacity of Tea and Common Vegetables. Journal of Agricultural and Food Chemistry, 44, 3426-3431.
[2] Wang, H., Cao, G. and Prior, R.L. (1996) Total Antioxidant Capacity of Fruits. Journal of Agricultural and Food Chemistry, 44, 701-705.
[3] Larson, R.A. (1988) The Antioxidants of Higher Plants. Phytochemistry, 27, 969-978.
[4] Dragsted, L.O. (2003) Antioxidant Actions of Polyphenols in Humans. International Journal for Vitamin and Nutrition Research, 73, 112-119.
[5] Hale, L.P., Greer, P.K., Trinh, C.T. and James, C.L. (2005) Proteinase Activity and Stability of Natural Bromelain Preparations. International Immunopharmacology, 5, 783-793.
[6] Taussig, S.J. and Batkin, S. (1988) Bromelain, the Enzyme Complex of Pineapple (Ananas comosus) and Its Clinical Application: An Update. Ethnopharmacol, 22, 191-203.
[7] Hale, L.P. (2004) Proteolytic Activity and Immunogenicity of Oral Bromelain within the Gastrointestinal Tract of Mice. International Immunopharmacology, 4, 255-264.
[8] Chakraborty, S., Rao, P.S. and Mishra, H.N. (2014) Effect of pH on Enzyme Inactivation Kinetics in High-Pressure Processed Pineapple (Ananas comosus L.) Puree Using Response Surface Methodology. Food and Bioprocess Technology, 7, 3629-3645.
[9] Shomer, R., Cogan, U. and Mannheim, C.H. (1994) Thermal Death Parameters of Orange Juice and Effect of Minimal Heat Treatment and Carbon Dioxide on Shelf-Life. Journal of Food Processing and Preservation, 18, 305-315.
[10] Devlieghere, F., Vermeiren, L. and Debevere, J. (2004) New Preservation Technologies: Possibilities and Limitations. International Dairy Journal, 14, 273-285.
[11] Jeyamkondan, S., Jayas, D.S. and Holley, R.A. (1999) Pulsed Electric field Processing of Foods: A Review. Journal of Food Protection, 62, 1088-1096.
[12] Bhat, R., Ameran, S.B., Karim, A.A. and Liong, M.T. (2011) Quality Attributes of Starfruit (Averrhoa carambola L.) Juice Treated with Ultraviolet Radiation. Food Chemistry, 127, 641-644.
[13] Tiwari, B.K., Muthukumarappan, K., O’Donnell, C.P. and Cullen, P.J. (2009) Inactivation Kinetics of Pectin Methylesterase and Cloud Retention in Sonicated Orange Juice. Innovative Food Science and Emerging Technologies, 10, 166-171.
[14] Valero, M., Recrosio, N., Saura, D., Munoz, N., Martí, N. and Lizama, V. (2007) Effects of Ultrasonic Treatments in Orange Juice Processing. Journal of Food Engineering, 80, 509-516.
[15] O’Donnell, C.P., Tiwari, B.K., Bourke, P. and Cullen, P.J. (2010) Effect of Ultrasonic Processing on Food Enzymes of Industrial Importance. Trends in Food Science & Technology, 21, 358-367.
[16] Cheng, L.H., Soh, C.Y., Liew, S.C. and Teh, F.F. (2007) Effects of Sonication and Carbonation on Guava Juice Quality. Food Chemistry, 104, 1396-1401.
[17] Tiwari, B.K., O’Donnell, C.P.O., Patras, A. and Cullen, P.J. (2008) Anthocyanin and Ascorbic Acid Degradation in Sonicated Strawberry Juice. Journal of Agriculture and Food Chemistry, 56, 10071-10077.
[18] Salleh-Mack, S.Z. and Roberts J.S. (2007) Ultrasound Pasteurization: The Effects of Temperature, Soluble Solids, Organic Acids and pH on the Inactivation of Escherichia coli ATCC 25922. Ultrasonics Sonochemistry, 14, 323-329.
[19] Ugarte-Romero, E., Feng, H., Martin, S.E., Cadwallader, K.R. and Robinson, S.J. (2006) Inactivation of Escherichia coli with Power Ultrasound in Apple Cider. Journal of Food Science, 71, 102-108.
[20] Baumann A.R., Martin S.E. and Feng H. (2005) Power Ultrasound Treatment of Listeria Monocytogenes in Apple Cider. Journal of Food Protection, 68, 2333-2340.
[21] Piyasena, P., Mohareb, E. and McKellar, R.C. (2003) Inactivation of Microbes Using Ultrasound. International Journal of Food Microbiology, 87, 207-216.
[22] Jiranek, V., Grbin, P., Yap, A., Barnes, M. and Bates, D. (2008) High Power Ultrasonics as a Novel Tool Offering New Opportunities for Managing Wine Microbiology, Biotechnology Letters, 30, 1-6.
[23] James, C.S. (1995) Analytical Chemistry of Foods. Springer, New York.
[24] Naresh, K., Varakumar, S., Variyar, P.S., Sharma, A. and Reddy, O.V.S. (2014) Impact of γ-Irradiation on Antioxidant Capacity of Mango (Mangifera indica L.) Wine from Eight Indian Cultivars and the Protection of Mango Wine against DNA Damage Caused by Irradiation. Process Biochemistry, 49, 1819-1830.
[25] Roig, M.G., Bello, J.F., Rivera, Z.S. and Kennedy, J.F. (1999) Studies on the Occurrence of Nonenzymatic Browning during Storage of Citrus Juice. Food Research International, 32, 609-619.
[26] Aguiló-Aguayo, I., Soliva-Fortuny, R. and Martín-Belloso, O. (2009) Changes in Viscosity and Pectolytic Enzymes of Tomato and Strawberry Juices Processed by High-Intensity Pulsed Electric fields. International Journal of Food Science and Technology, 44, 2268-2277.
[27] Alothman, M., Bhat, R. and Karim, A.A. (2009) Antioxidant Capacity and Phenolic Content of Selected Tropical Fruits from Malaysia, Extracted with Different Solvents. Food Chemistry, 115,785-788.
[28] Bhardwaj, R.L. and Pandey, S. (2011) Juice Blends—A Way of Utilization of Under-Utilized Fruits, Vegetables, and Spices: A Review. Critical Reviews in Food Science and Nutrition, 51, 563-570.
[29] Costa, M.G., Fonteles, T.V., de Jesus, A.L. and Rodrigues, S. (2013) Sonicated Pineapple Juice as Substrate for L. casei Cultivation for Probiotic Beverage Development: Process Optimisation and Product Stability. Food Chemistry, 139, 261-266.
[30] Cansino, N.C., Carrera, G.P., Rojas, Q.Z., Olivares, L.D., García, E.A. and Moreno, E.R. (2013) Ultrasound Processing on Green Cactus Pear (Opuntia ficus Indica) Juice: Physical, Microbiological and Antioxidant Properties. Journal of Food Processing and Technology, 4, 1-6.
[31] Joslyn, M.A. and Ponting, J.D. (1951) Enzyme-Catalyzed Oxidative Browning of Fruit Products. Advances in Food Research, 3, 1-44.
[32] Ibarz, A., Pagan, J. and Garza, S. (2000) Kinetic Models of Non-Enzymatic Browning in Apple Puree. Journal of the Science of Food and Agriculture, 80, 1162-1168.
[33] Landl, A., Abadias, M., Sárraga, C., Vinas, I. and Picouet, P.A. (2010) Effect of High Pressure Processing on the Quality of Acidified Granny Smith Apple Purée Product. Innovative Food Science and Emerging Technologies, 11, 557-564.
[34] Sapers G.M., Miller R.L., Pilizota V. and Kamp, F. (2001) Shel-Flife Extension of Fresh Mushrooms (Agaricus bisporus) by Application of Hydrogen Peroxide and Browning Inhibitors. Journal of Food Science, 66, 362-366.
[35] Espin, J.C., Jolivet, S. and Wichers, H.J. (1998) Inhibition of Mushroom Polyphenol Oxidase by Agaritine. Journal of Agricultural and Food Chemistry, 46, 2976-2980.
[36] Murr, D.P. and Morris, L.L. (1974) Influence of O2 and CO2 on O-Diphenol Oxidase Activity in Mushroom. Journal of the American Society for Horticultural Science, 99, 155-158.
[37] Gupta, P., Maqbool, T. and Saleemuddin, M. (2007) Oriented Immobilization of Stem Bromelain via the Lone Histidine on a Metal Affinity Support. Journal of Molecular Catalysis B: Enzymatic, 45, 78-83.
[38] Poh, S.S. and Abdul Majid, F.A. (2011) Thermal Stability of Free Bromelain and Bromelainpolyphenol Complex in Pineapple Juice. International Food Research Journal, 18, 1051-1060.
[39] Sew, C., Ghazali, H., Martín-Belloso, O. and Noranizan, M. (2014) Effects of Combining Ultraviolet and Mild Heat Treatments on Enzymatic Activities and Total Phenolic Contents in Pineapple Juice. Innovative Food Science and Emerging Technologies, 26, 511-516.
[40] Goh, S.G., Noranizan, M., Leong, C.M., Sew, C.C. and Sobhi, B. (2012) Effect of Thermal and Ultraviolet Treatments on the Stability of Antioxidant Compounds in Single Strength Pineapple Juice Throughout Refrigerated Storage. International Food Research Journal, 19, 1131-1136.
[41] Khandpur, P. and Gogate, P.R. (2015) Effect of Novel Ultrasound Based Processing on the Nutrition Quality of Different Fruit and Vegetable Juices. Ultrasonics Sonochemistry, 27, 125-136.
[42] Macheix, J., Fleuriet, A. and Billot, J. (1990) Fruit Phenolics. CRC Press, Boca Raton, 378.
[43] Chakraborty, S., Rao, P. and Mishra, H. (2015) Effect of Combined High Pressure-Temperature Treatments on Color and Nutritional Quality Attributes of Pineapple (Ananas comosus L.) Puree. Innovative Food Science and Emerging Technologies, 28, 10-21.
[44] Shamsudin, R., Noranizan, M.A., Yap, P.Y. and Mansor, A. (2014) Effect of Repetitive Ultraviolet Irradiation on the Physic-Chemical Properties and Microbial Stability of Pineapple Juice. Innovative Food Science and Emerging Technologies, 23, 114-120.
[45] Shamsudin, R., Chia, S.L., Mohd Adzahan, N. and Wan Daud, W.R. (2013) Rheological Properties of Ultraviolet-Irradiated and Thermally Pasteurized Yankee Pineapple Juice. Journal of Food Engineering, 116, 548-553.
[46] Butz, P. and Tauscher, B. (2002) Emerging Technologies: Chemical Aspects. Food Research International, 35, 279-284.
[47] Fellows, P. (2000) Food Processing Technology: Principles and Practice. 2nd Edition, CRC Press, New York.
[48] Cao, S., Hu, Z. and Pang, B. (2010) Optimization of Postharvest Ultrasonic Treatment of Strawberry Fruit. Postharvest Biology and Technology, 55, 150-153.
[49] Chen, Z. and Zhu, C. (2011) Combined Effects of Aqueous Chlorine Dioxide and Ultrasonic Treatments on Postharvest Storage Quality of Plum Fruit (Prunus salicina L.). Postharvest Biology and Technology, 61, 117-123.
[50] Dolatowski, Z.J. and Stasiak, D.M. (2002) Czystosc mikrobiologiczna miesa i szynki parzonej poobróbce ultradzwiekowej [Bacterial Contamination of Meat and Meat Products after Ultrasound Treatment]. Acta Scientiarum Polonorum Technologia Alimentaria, 1, 55-65. (In Polish)

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