Phenolic Compositions of Litchi Shoot Tips and Zygotic Embryos Collected in Different Months and Their Effects on the Explant Browning and Its Control

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DOI: 10.4236/aa.2016.64008    425 Downloads   569 Views  
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ABSTRACT

In the research it was examined the changes in total phenolic contents and seven major phenolic compounds (gallic acids, +() catechin, catechol, Chlorogenic acid, o-coumaric acid, rutin and quercetin) of two litchi cultivars (Purbi and Bedana) shoot tips and fruits (for zygotic embryos) collected in different months, in order to determine their effects on the explants browning during establishment stage of shoot tip culture. The concentrations of phenolic compounds varied depending on the cultivars and the months. Phenolic compounds showed various correlation coefficients with the explants browning. Total phenolic content and some individual phenolic compounds including +() catechin, catechol, gallic acid, chlorogenic acid and rutin quantified in this study showed significant positive correlations with the explants browning, while o-coumaric acid and quercetin did not exhibit any significant one. According to our results, explants browning are affected by the phenolic compounds at different ranges. In both litchi cultivars, shoot tips and fruits (for zygotic embryos) collected in March exhibited the lowest explants browning during the establishment stage as compared to those collected in the other months. So it may be possible to increase the success of shoot tip and zygotic embryo culture with the selection of the most suitable terms of explants collection. Browning of explants could be controlled by the use of antioxidants both in semi-solid and liquid culture.

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Das, D. and Rahman, A. (2016) Phenolic Compositions of Litchi Shoot Tips and Zygotic Embryos Collected in Different Months and Their Effects on the Explant Browning and Its Control. Advances in Anthropology, 6, 73-92. doi: 10.4236/aa.2016.64008.

References

[1] Benson, E. E., & Roubelakis-Angelakis, K. A. (1992). Oxidative Stress in Recalcitrant Tissue Cultures of Grapevine. Plant Science, 84, 83-90.
http://dx.doi.org/10.1016/0168-9452(92)90211-4
[2] Bhat, S. R., & Chandel, K. P. S. (1991) A Novel Technique to Overcome Browning in Tissue Culture. Plant Cell Reports, 10, 358-361.
http://dx.doi.org/10.1007/BF00193159
[3] Block, R., & Lankes, C. (1995). Gartenbauwissenschaft, 60, 276-279.
[4] Chanana, Y. R., & Gill, M. I. S. (2008). General Horticulture, Propagation and Nursery Management.
[5] Chandra, R., & Padari, J. C. (1999). Litchi Shoots Bud Culture for Micropropagation. Journal of Applied Horticulture, 1, 38-40.
[6] Chew, P. C., Mardaleni Normah, M. N., & Clyde, M. M. (2008). Activated Charcoal Is Crucial for Successful Micropropagation of Rambutan (Nephelium lappaceum Linn.). Malaysian Applied Biology, 37, 11-20.
[7] Dalal, M. A., Sharma, B. B., & Rao, M. S. (1992). Mode in Control of Oxidative Browning in Vitro Cultures of Grapevine. Scientia Horticulturae, 51, 35-41.
http://dx.doi.org/10.1016/0304-4238(92)90101-H
[8] Dalal, M. A., Sharma, B. B., & Grupta, N. (1993). In Vitro Regeneration of a Mature Leguminous Lians (Bauhinia vahli Wight and Arnott). Indian Journal of Agricultural Sciences, 63, 75-79.
[9] Das, D. K., Shiva Prakash, N., & Sarin, N. B. (1999a) Multiple Shoot Induction and Plant Regeneration of Litchi (Litchi chinensis Sonn.). Plant Cell Reports, 18, 619-695.
http://dx.doi.org/10.1007/s002990050644
[10] Das, D. K., Shiva Prakash, N., & Sarin, N. B. (1999b). Multiple Shoot Induction in Litchi chinensis: A Tropical Tree Species. In P. B. Kavi Kishore (Ed.), Plant Tissue Culture and Biotechnology: An Emerging Trends (pp. 166-169). Hyderabad: Sangam Books Ltd.
[11] Das, D. K., Reddy, M. K., Upadhyaya, K. C., & Sopory, S. K. (2002). An Efficient Leaf Disc Culture Method for Regeneration via Somatic Embryogenesis and Transformation of Grape (Vitis vinifera L.). Plant Cell Reports, 20, 999-1005.
http://dx.doi.org/10.1007/s00299-002-0441-4
[12] Dhar, U., & Upreti, J. (1999). In Vitro Regeneration of a Mature Leguminous Liana (Bauhinia vahli Wight and Arnott). Plant Cell Reports, 18, 664-669.
http://dx.doi.org/10.1007/s002990050639
[13] Fanizza, G., Tanzarella, O. A., Carrozzo, G., & Greco, B. (1984). Methylated DNA Changes Associated with the Initiation and Maintenance of Vitis vinifera in Vitro Shoot and Callus Cultures: A Possible Mechanism for Age Related Changes. Annals of Applied Biology, 104, 577-578.
http://dx.doi.org/10.1111/j.1744-7348.1984.tb03042.x
[14] Gannoun, S., Lionakis, S. M., & Gerasopoulos, D. (1995). Aspects of in Vitro Culture of Pistacia terebinthus and Pistacia vera. In N. Kaska, A. B. Küden, L. Ferguson, & T. Michailides (Eds.), I International Symposium on Pistachio. ISHS Acta Horticulturae 419.
http://dx.doi.org/10.17660/actahortic.1995.419.32
[15] Harding, K., & Roubelakis-Angelakis, K. A. (1994). The Isolation and Purification of DNA from Vitis vinifera L. Plants and In-Vitro Cultures. Vitis, 33, 247-248.
[16] Hu, C. Y., & Wang, P. J. (1983). D. A. Evans, W. R. Sharp, P. V. Ammirato, & Y. Yamada (Eds.), Handbook of Plant Cell Culture (Vol. 1, pp. 177-227). New York: Macmillan.
[17] Kantharajah, A. S., McConchie C. A., & Dodd W. A. (1992). In Vitro Embryo Culture and Induction of Multiple Shoots in Lychee (Litchi chinensis Sonn.). Annals of Botany, 70, 153-156.
[18] Karkacier, M. (1998). Trabzon Hurmasimin (Diospyros kaki L.) Kimyasal Bile Imifizerinearafirmalar (p. 95). PhD Thesis, Ankara: Ankara Universitesi Fen Bilimleri Enstitusu.
[19] Kotsias, D., & Roussos, P. A. (2001). An Investigation on the Effect of Different Plant Growth Regulatory Compounds in in-Vitro Shoot Tip and Node Culture of Lemon Seedlings. Scientia Horticulturae, 89, 115-128.
http://dx.doi.org/10.1016/S0304-4238(00)00227-2
[20] Krishna, H. (2008). Mango Tissue Culture, Constraints and Prospects. Agricultural and Biological Sciences.
[21] Lux-Endrich, A., Treutter, D., & Feucht, W. (2000). Influence of Nutrients and Carbohydrate Supply on the Phenol Composition of Apple Shoots Culture. Plant Cell, Tissue and Organ Culture, 60, 15-21.
http://dx.doi.org/10.1023/A:1006406527242
[22] Mishra, D. S., Tiwari, J. P., & Shant, L. (2007). In Vitro Cloning of Guava (Psidium guajava L.) cv. Pant Prabhat. ISHS Acta Horticulturae, 735, I53-157.
http://dx.doi.org/10.17660/actahortic.2007.735.16
[23] Murashige, T., & Skoog, F. (1962). A Revised Medium Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
[24] Olhoft, P. M., & Somers, D. A. (2001). L-Cysteine Increases Agrobacterium Mediated T-DNA Delivery into Soybean Cotyledonary-Node Cells. Plant Cell Reports, 20, 706-711.
http://dx.doi.org/10.1007/s002990100379
[25] Puchooa, D. (2004). In Vitro Regeneration of Lychee (Litchi chinensis Sonn.). African Journal of Biotechnology, 3, 576-584.
[26] Rasar, S., George, A. P., & Kantharajah, A. S. (1995). Tissue Culture of Annona spp. (Chenimoya Atemoya, Sugar Apple and Soursop): A Review. Scientia Horticulturae, 62, 1-14.
http://dx.doi.org/10.1016/0304-4238(94)00758-8
[27] Richard-Forget, C. F., Ronet-Mayer, M., Goupy, M. P., Philippon, J., & Nicolas, J. J. (1992) Cysteine as an Inhibitor of Enzymatic Browning II Kinetic Studies. Journal of Agricultural and Food Chemistry, 40, 2114-2122.
http://dx.doi.org/10.1021/jf00023a015
[28] Roussos, P. A., & Pontikis, C. A. (2001a). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Journal of Horticultural Science and Biotechnology, 76, 441-446.
http://dx.doi.org/10.1080/14620316.2001.11511391
[29] Roussos, P. A., & Pontikis, C. A. (2001b) Phenolic Compounds in Olive Explants and Their Contributions to Browning during the Establishment Stage in Vitro. Gartenbauwissenschaft, 66, 298-303.
[30] Rouston, J. P., Latche, A., & Fallot, J. (2008). Enhancement of Shoot Regeneration from Cotyledons of Cucumis melo by AgNO3, an Inhibitor of Ethylene Action. Journal of Plant Physiology, 140, 485-488.
http://dx.doi.org/10.1016/S0176-1617(11)80830-8
[31] Sarin, N. B., Prasad, U. S., Kumar, M., & Jain, S. M. (2008). Litchi Breeding for Genetic Improvement. In S. Mohan Jain, & P. M. Privadarshan (Eds.), Breeding Plantation Tree Crops, Tropical Species (pp. 217-245). Berlin: Springer.
[32] Singleton, V. L., & Rossi, J. R. (1965). Shoot Tip Culture in Mango, Influence of Medium Genotype Explants Factors, Seasons and Decontamination on Phenolic Exudation Explants Survival and Axenic Culture Establishment. American Journal of Enology and Viticulture, 16, 144-158.
[33] Sun, J., Xiang, X., Yu, C. Y., Shi, J. Y., Peng, H. X., Yang, B., Yang, S. Y., Yang, E., & Jiang, Y. (2009). Variations in Contents of Browning Substrates and Activities of Some Related Enzymes during Litchi Fruit Development. Scientia Horticulturae, 120, 555-559.
http://dx.doi.org/10.1016/j.scienta.2008.12.006
[34] Tabiyeh, D. T., Bernard, F., & Shacker, H. (2006). Investigation of Glutathione, L-Cysteine, Sajlicylic Acid and GA3 Effects on Browning in Pistacia vera Shoot Tips Culture. In A. Javanshah, E. Facelli, & M. Wirthensohn (Eds.), IV International Symposium on Pistachios and Almonds. ISHS Acta Horticulturae 726.
[35] Tangolar, S., Buyukia, N., Gok, S., & Ergenoglu, F. (1999). Turkiye III (pp. 383-388). Ulusal Bahce Bitkileri Kongresi, 14-17 Eylul 1999, Ankara.
[36] Thomas, P., & Ravindra, M. B. (1997). Shoot Tip Culture in Mango; Influence of Medium, Genotype, Explant Factors, Season and Decontamination Treatments on Phenolic Exudation, Explant Survival and Axenic Culture Establishment. Horticultural Science, 72, 713-722.
http://dx.doi.org/10.1080/14620316.1997.11515563
[37] Toth, K., Haapala, T., & Hohtola, A. (1993) Alleviation of Browning in Oak Explants by Chemical Pretreatments. Biologia Plantarum, 36, 511-517.
http://dx.doi.org/10.1007/BF02921170
[38] Yu, D., & Meredith, C. P. (1986). The Influence of Explants Origin on Tissues Browning and Shoot Production in Shoot Tip Cultures of Grapevine. Journal of the American Society for Horticultural Science, 111, 972-975.
[39] Ziv, M., & Halevy, A. H. (1983). Control of Oxidative Browning and in Vitro Propagation of Shaliziareginae. Horticultural Science, 18, 434-436.

  
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