Sonodynamic Antitumor Effect of Benzoporphyrin Derivative Monoacid Ring A on KLN205 Cells

Tomohiro Osaki, Motoshi Tajima, Yoshiharu Okamoto, Satoshi Takagi, Takeshi Tsuka, Tomohiro Imagawa, Saburo Minami

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DOI: 10.4236/jct.2011.22011                

Abstract

Sonodynamic therapy is a new cancer treatment based on the synergetic effect of ultrasound and a drug. In this study, ultrasonically induced antitumor effects of benzoporphyrin derivative monoacid ring A (BPD-MA) on KLN205 cells were investigated. KLN205 cells were irradiated at an ultrasonic frequency of 3 MHz with 10 μg/ml BPD-MA. The ultrasonically induced cell damage significantly increased as an ultrasonic intensity and ultrasound exposure time increased. Confocal microscopic examination revealed that the irradiated cells were induced chromatin condensation and phosphatidylserine exposure. The synergistic effect of the ultrasound exposure and BPD-MA on the tumor cell adhesion rate was significant.

Keywords

BPD-MA, Cell Damage, Sonodynamic Therapy, Sonosensitizer, Ultrasound

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	N. Yumita, R. Nishigaki, K. Umemura and S. Umemura, “Synergistic Effect of Ultrasound and Hematoporphyrin on Sarcoma 180,” Japanese Journal of Cancer Research, Vol. 81, No. 3, March 1990, pp. 304-308.
[2]	N. Yumita, R. Nishigaki, K. Umemura and S. Umemura, “Hematoporphyrin as a Sensitizer of Cell-Damaging Effect of Ultrasound,” Japanese Journal of Cancer Research, Vol. 80, No. 3, March 1989, pp. 219-222.
[3]	I. Rosenthal, J. Z. Sostaric and P. Riesz, “Sonodynamic Therapy—A Review of the Synergistic Effects of Drugs and Ultrasound,” Ultrason Sonochem, Vol. 11, No. 6, September 2004, pp. 349-363.
[4]	A. H. Saad and G. M. Hahn, “Ultrasound-Enhanced effects of Adriamycin against Murine Tumors,” Ultrasound in Medicine and Biology, Vol. 18, No. 8, 1992, pp. 715-723. doi:10.1016/0301-5629(92)90122-Q
[5]	G. H. Harrison, E. K. Alcer-Kubiczek and P. L. Gutierrez, “In Vitro Mechanisms of Chemopotentiation by Tone- Burst Ultrasound,” Ultrasound in Medicine and Biology, Vol. 22, No. 3, 1996, pp. 355-362. doi:10.1016/0301-5629(95)02053-5
[6]	Q. Liu, X. Li, L. Xiao, P. Wang, X. Wang and W. Tang, “Sonodynamically Induced Antitumor Effect of Hematoporphyrin on Hepatoma 22,” Ultrasonics Sonochemistry, Vol. 15, No. 6, September 2008, pp. 943-948. doi:10.1016/j.ultsonch.2008.04.001
[7]	T. J. Dougherty, C. J. Gomer, B. W. Henderson, G. Jori, D. Kessel, M. Korbelik, J. Moan and Q. J. Peng, “Photodynamic Therapy,” Journal of the National Cancer Institute, Vol. 90, No. 12, June 1998, pp. 889-905. doi:10.1093/jnci/90.12.889
[8]	T. J. Dougherty, “An Update on Photodynamic Therapy Applications,” Journal of Clinical Laser Medicine and Surgery, Vol. 20, No. 1, February 2002, pp. 3-7. doi:10.1089/104454702753474931
[9]	L. Ayaru, S. G. Bown and S. P. Pereira, “Photodynamic Therapy for Pancreatic and Biliary Tract Carcinoma,” International Journal of Gastrointestinal Cancer, Vol. 35, No. 1, 2005, pp. 1-13. doi:10.1385/IJGC:35:1:001
[10]	A. M. Richter, S. Cerruti-Sola, E. D. Sternberg, D. Dolphin and J. G. Levy, “Biodistribution of Tritiated Benzoporphyrin Derivative (3H-BPD-MA), a Newpotent Photosensitizer, in Normal and Tumorbearing Mice,” Journal of Photochemistry and Photobiology B, Vol. 5, No. 2, April 1990, pp. 231-244. doi:10.1016/1011-1344(90)80008-L
[11]	G. I. Stables and D. V. Ash, “Photodynamic Therapy,” Cancer Treatment Reviews, Vol. 21, No. 4, July 1995, pp. 311-323. doi:10.1016/0305-7372(95)90035-7
[12]	D. Huang, K. Okada, K. Comori, E. Itoi and T. Suzuki. “Enhanced Antitumor Activity of Ultrasonic Irradiation in the Presence of New Quinolone Antibiotics in Vitro,” Cancer Science, Vol. 95, No. 10, October 2004, pp. 845-849. doi:10.1111/j.1349-7006.2004.tb02192.x
[13]	J. H. Li, D. Y. Song, Y. G. Xu, Z. Huang and W. Yue, “In Vitro Study of Haematoporphyrin Monomethyl Ether- Mediated Sonodynamic Effects on C6 Glioma Cells,” Neurological Sciences, Vol. 29, No. 4, September 2008, pp. 229-235. doi:10.1007/s10072-008-0972-8
[14]	J. M. Runnels, N. Chen, B. Ortel, D. Kato and T. Hasan, “BPD-MA-Mediated Photosensitization in Vitro and in Vivo: Cellular Adhesion and β1 Integrin Expression in Ovarian Cancer Cells,” British Journal of Cancer, Vol. 80, No. 7, June 1999, pp. 946-953. doi:10.1038/sj.bjc.6690448
[15]	J. F. Tait, “Imaging of Apoptosis,” Journal of Nuclear Medicine, Vol. 49, No. 10, September 2008, pp. 1573-1576. doi:10.2967/jnumed.108.052803
[16]	M. van Engleland, L. J. Nieland, F. C. Ramaekers, B. Schutte and C. P. Reutelingsperger, “Annexin V-affinity Assay: A Review on an Apoptosis Detection System Based on Phosphatidylserine Exposure,” Cytometry, Vol. 31, No. 1, January 1998, pp. 1-9. doi:10.1002/(SICI)1097-0320(19980101)31:1<1::AID-CYTO1>3.0.CO;2-R
[17]	Q. Liu, X. Wang, P. Wang, H. Qi, K. Zhang and L. Xiao, “Sonodynamic Effects of Protoporphyrin IX Disodium Salt on Isolated Sarcoma 180 Cells,” Ultrasonics, Vol. 45, No. 1-4, July 2006, pp. 56-60. doi:10.1016/j.ultras.2006.06.063
[18]	L. D. Barnes, E. A, Gluliano, J. Ota, L. A. Cohn and C. P. Moore, “The Effect of Photodynamic Therapy on Squa- mous Cell in a Murine Model: Evaluation of Time Between Intralesional Injection to Laser Irradiation,” The Veterinary Journal, Vol. 180, No. 1, February 2008, pp. 60-65. doi:10.1016/j.tvjl.2007.11.023
[19]	P. Chiarugi and E. Giannoni, “Anoikis: A Necessary Death Program for Anchorage-Dependent Cells,” Biochemical Pharmacology, Vol. 76, No. 11, July 2008, pp. 1352-1364. doi:10.1016/j.bcp.2008.07.023
[20]	G. Melino, R. A. Knight and P. Nicotera, “How Many Ways to Die? How Many Different Models of Cell Death?” Cell Death & Differentiation, Vol. 12, November 2005, pp. 1457-1462. doi:10.1038/sj.cdd.4401781
[21]	S. Umemura, N. Yumita, R. Nishigaki and K. Umemura, “Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin,” Japanese Journal of Cancer Research, Vol. 81, No. 9, September 1990, pp. 962-966.
[22]	N. Yumita, N. Okuyama, K. Sasaki and S. Umemura, “Sonodynamic Therapy on Chemically Induced Mammary Tumor: Pharmacokinetics, Tissue Distribution and Sonodynamically Induced Antitumor Effect of Porfimer Sodium,” Cancer Science, Vol. 95, No. 9, September 2004, pp. 765-769. doi:10.1111/j.1349-7006.2004.tb03259.x
[23]	M. W. Miller, D. L. Miller and A. A. Brayman, “A Review of in Vitro Bioeffects of Inertial Ultrasonic Cavitation from a Mechanistic Perspective,” Ultrasound in Medicine and Biology, Vol. 22, No. 9, 1996, pp. 1131- 1154. doi:10.1016/S0301-5629(96)00089-0
[24]	S. Umemura, N. Yumita and R. Nishigaki, “Enhancement of Ultrasonically Induced Cell Damage by a Gallium- Porphyrin Complex, ATX-70,” Japanese Journal of Cancer Research, Vol. 84, No. 5, May 1993, pp. 582-588.