Evaluation of the Effects of Corticosteroids on Histamine Release by ex Vivo Cutaneous Microdialysis

Carol Courderot-Masuyer, Sophie Robin, Hélène Tauzin, Sylvain Harbon, Sophie Mac-Mary, Alexandre Guichard, Patrice Muret, Philippe Humbert

Bioexigence SARL, Espace Lafayette, 8 rue Alfred de Vigny, Besan?on, France.
Laboratory of Clinical and Toxicology Pharmacology, Jean Minjoz University Hospital, Besancon, France.
Plastic Surgery, 9 rue Klein, Saint Vincent Clinic, Besan?on, France.
Research and Clinicl Center on the Tegument (CERT); Clinical Investigation Center (CIC BT506), Department of Dermatology, Besan?on University Hospital, Besan?on, France.
Skinexigence SAS, immeuble Bioparc, 2 rue du Dr Paul Milleret, Besan?on, France.
University of Franche-Comté, Inserm U1098, SFR FED 4234 IBCT, Besan?on, France.

DOI: 10.4236/jcdsa.2013.33035                

Abstract

The purpose of the study was to evaluate the effects of corticosteroids on histamine release and to compare their potency with the MacKenzie classification based on their vasoconstrictor effects. Thanks to ex Vivo cutaneous microdialysis, we studied histamine-induced release over a period of time on excised abdominal skin from women. Eight corticosteroids were topically applied with occlusive dressing onto the skin, above probes, before anti-IgE injection. Histamine levels were assessed by an EIA method. In order to compare the different corticosteroids, AUC was calculated allowing an estimation of the amount of released histamine for 60 min of ex vivo cutaneous microdialysis. Diflucortolone 0.1% and micronized betamethasone dipropionate 0.05% are considered as corticosteroids with high potency in MacKenzie classification. Betamethasone dipropionate associated with propylene glycol 0.05%, belongs to a stronger class in Mackenzie classification. Our results showed that the decrease in histamine release was more important with difluocortolone than with both of these corticosteroids. Therefore there was no correlation between the vasoconstrictor potency of topical corticosteroids and their ability to inhibit histamine release.

Keywords

MacKenzie Test; Corticosteroids; ex Vivo Cutaneous Microdialysis; Histamine

Conflicts of Interest

The authors declare no conflicts of interest.

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