TITLE:
Effect of Cumulus Cells on the Efficiency of Vitrified-Thawed Immature Cattle Oocytes
AUTHORS:
Samia Mohamed Abd-El Rheem, Suzan Elsharkawy
KEYWORDS:
Cumulus Cells, Vitrified-Thawed Immature Oocytes, Cattle
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.9 No.5,
May
21,
2019
ABSTRACT: The present work was
designed to examine the effect of the presence or absence of cumulus cells on
the efficiency of vitrification of immature cattle oocytes. In our experiment, we had two groups: group 1, immature cattle oocytes
with cumulus cells and group 2, immature cattle oocytes without cumulus cells. The two groups underwent vitrification using 20%
ethylene glycol and 20% DMSO, and then thawed, and in vitro matured in TCM-199
medium and examined after 22 hours for assessment of nuclear maturation. Higher survival rate (p in-vitro maturation, the rate of MII oocytes was significantly higher (p , the cumulus cells are very important in increasing the survivability and
developmental rate of vitrified-thawed immature cattle oocytes.