TITLE:
Impact of Antibacterial Activity of Physical Storage Extracts on Pathogenic Bacteria
AUTHORS:
Ashwag Al-Zahrani, Hanan Omer, Awatif Al-Judaibi
KEYWORDS:
Resistant Bacteria, S. aureus, P. aeruginosa, U. lactuca, N. sativa, Kill-Time
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.4 No.4,
April
22,
2016
ABSTRACT: The overuse of
antibiotics can lead to resistance among pathogenic bacteria. A new antibiotic
that is effective against new and resistant bacterial strains is needed. Plants
and marine organisms may offer such novel treatments. In this study, extracts
of the seaweed U. lactuca, and the plant seeds N. sativa were tested against strains of Gram-positive
cocci and Gram-negative bacilli S. aureus, and P. aeruginosa. The
results of the bacterial inhibitor showed high activity in both extracts with
inhibition of S. aureus growth up to 30 mm and 20 mm and P. aeruginosa growth
inhibition was up to 12 mm and 15 mm, after the treated with 100 μl U. lactuca and N. sativa extracts, respectively. The MICs and MBCs were
reflected with the growth inhibitor with values of 2 μl, 8 μl and 4 μl, 8 μl
for S. aureus and P. aeruginosa after treated with N. sativa respectively.
Kill-time increases as concentrations of U. lactuca and N. sativa extracts increase. Moreover, extracts stored in the transparent bottle
decreased in effectiveness after one month of storage with percentage of
58.85%. After three months, heating the extracts of U. lactuca and N.
sativa to 90°C increased their antibacterial activity.