TITLE:
Bioconversion of Fish Hatchery Waste as Feed in the Production of Live Feed
AUTHORS:
Sujjat Al Azad, Al-Najib Bin Benjamin, Mohammad Tamrin Bin Mohamad Lal
KEYWORDS:
Hatchery Waste, Bioprocess, Microbe and Live Hatchery Waste, Bioprocess, Microbe and Live Feed Growth
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.11 No.7,
July
13,
2020
ABSTRACT: Purple Non-Sulfur Bacteria (PNSB), also known as
phototrophic bacteria are widely distributed in both freshwater and marine
environment and capable to grow in wide
range of substrates. In this study, Bacterium Rhodobacter sphaeroides strain UMS2, a freshwater isolate was used in this study in
utilization of fish hatchery waste. This study was conducted to determine the
nutritional values of bioprocess product that was grown in fish hatchery waste.
Finally, the waste bio-converted product was used as feed supplement to monitor
the growth performance of live feed Tubifex spp. Inoculum of Rhodobacter sphaeroides strain UMS2 was developed in 112 synthetic media and
48-h culture of 30% (v/v) inoculum was used in fish hatchery waste during the
bioprocess. The nutritional values of bio-converted product, except total ash
(%), were not significantly improved with 30% (v/v) inoculum of Rhodobacter sphaeroides, strain UMS2.
Feeding trial in bloodworm (Tubifex spp.) with bioconversion product conducted for 15 days to monitor growth (w/v)
of live feed. Initial growth 1.42 ± 0.001 g/L of Tubifex spp. was
stocked in 15 × 75 × 15 cm plastic tray connected with recirculated system. Tubifex spp. was observed to be
comparatively higher (1.55 ± 0.12 g/L) while fed in the product that contained
bacterium than the growth (1.44 ± 0.15 g/L) of Tubifex spp. fed in the bioconversion product of without
bacterium. The inoculums size (30%) of bacterium
not enough to support the growth of Rhodobacter
sphaeroides, strain UMS2 in the bioconversion process to improve the
nutritional values. However, while used as feed supplement it improved the
growth performance of the Tubifex spp. So, bacterium Rhodobacter
sphaeroides, strain UMS2 has potentiality to be used as feed supplement in
the production of live feed.