TITLE:
Loop Structures and Barrier Elements from D. melanogaster 87А7 Heat Shock Locus
AUTHORS:
Mikhail V. Glazkov, Anna N. Shabarina
KEYWORDS:
hsp70 Genes, DNA-DNA Interactions, Insulators, Nuclear Envelope, TADs
JOURNAL NAME:
Computational Molecular Bioscience,
Vol.6 No.4,
December
5,
2016
ABSTRACT: The three-dimensional organization of the genome is closely related to its functioning. Interactions between parts of the genome located at large distances from each other have been detected within the chromosomes of different organisms, which led to the discovery of topologically associated domains (TADs). Methods that reveal such interactions between chromosomal loci imply detection of both protein-protein and protein-DNA interactions. We investigated the possibility of involvement of the direct DNA-DNA interactions in the structural and functional organization of Drosophila melanogaster chromosomal 87A7 locus, containing genes hsp70Aa and hsp70Ab, with the sequence analysis method. Our results indicate that the functional organization of 87A7 locus may involve different elements: chromosomal DNA fragments that attach chromosomes to the nuclear envelope, short polypurine/polypyrimidine tracts, insulators and their proteins. The combination of interactions of these elements may cause different functional states of 87A7 locus.